General information for g13527.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Sphingolipid delta(4)-desaturase DES1.
Parent gene
- The parent gene g13527 is located on chr_1: 30722460..30723456 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g13527 | g13527.t4 | TSS | g13527.t4 | 30721237 | 30721237 |
| chr_1 | g13527 | g13527.t4 | isoform | g13527.t4 | 30721732 | 30722194 |
| chr_1 | g13527 | g13527.t4 | exon | g13527.t4.exon1 | 30721732 | 30722194 |
| chr_1 | g13527 | g13527.t4 | cds | g13527.t4.CDS1 | 30721734 | 30722135 |
| chr_1 | g13527 | g13527.t4 | TTS | g13527.t4 | 30723064 | 30723064 |
Sequences
>g13527.t4 Gene=g13527 Length=463
CAATGGCAAATCGACTTTTTGGATTTTTCTGTAATCTTCCACTCGGCGTTCCAATGTCAA
TTTCTTTTAAAAGATATCATTTGGAACATCACAGATATCAGGGAGATGAAGTGCTTGATA
CTGATTTGCCTACACTTCTTGAAGCAAAATTATTTTGTACAACTTTTGGAAAATTTTGTT
GGGTTTGTTTGCAGCCATTCTTTTACCTTTTTCGTCCATTGGTCGTTAATCCAAAGAAAC
CATGCAAGCTTGAAGTTGTTAACACAATCATTCAATTGATCTTTGACTCGATAATCGTTT
ATGTTTTCGGTTGGAAAATGCTTGTTTATCTGATCATGGGTACATTGCTAGCTATGGGTT
TACATCCTGTCGCTGGTAATTTTGAACAAATCAAATGCTATTAATTATTCACTATTAAAA
CTTTTATTTTTTTAACATGTAGGTCATTTTATTAGTGAACACT
>g13527.t4 Gene=g13527 Length=133
MANRLFGFFCNLPLGVPMSISFKRYHLEHHRYQGDEVLDTDLPTLLEAKLFCTTFGKFCW
VCLQPFFYLFRPLVVNPKKPCKLEVVNTIIQLIFDSIIVYVFGWKMLVYLIMGTLLAMGL
HPVAGNFEQIKCY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 1 | g13527.t4 | PANTHER | PTHR12879 | SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2 | 2 | 127 | 7.5E-50 |
| 6 | g13527.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 47 | - |
| 7 | g13527.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 48 | 70 | - |
| 3 | g13527.t4 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 71 | 81 | - |
| 9 | g13527.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 82 | 102 | - |
| 5 | g13527.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 103 | 107 | - |
| 8 | g13527.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 108 | 127 | - |
| 4 | g13527.t4 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 128 | 133 | - |
| 2 | g13527.t4 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 97 | 119 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
