Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Glutathione S-transferase T1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13887 g13887.t1 TTS g13887.t1 33102224 33102224
chr_1 g13887 g13887.t1 isoform g13887.t1 33102279 33103129
chr_1 g13887 g13887.t1 exon g13887.t1.exon1 33102279 33102883
chr_1 g13887 g13887.t1 cds g13887.t1.CDS1 33102279 33102883
chr_1 g13887 g13887.t1 exon g13887.t1.exon2 33103012 33103129
chr_1 g13887 g13887.t1 cds g13887.t1.CDS2 33103012 33103129
chr_1 g13887 g13887.t1 TSS g13887.t1 33103158 33103158

Sequences

>g13887.t1 Gene=g13887 Length=723
ATGGCAAAATCATTTAAAATTTTTTACGACCATTTCTCACCACTTTCTAGAAGTCTATTA
ATCTTGTTTGAAAGGAATAATGTAGAATGTGAAAAAATATCTGTGGCATTAAGAAAGGGT
GAGCATCTTAGTGAAGAGTTTGGGGATAAAATAAATCGCTTTCATAAGGTACCTGTGATT
CATGATAGTGATGGATTTCGTTTAGCTGAATCAATAGCAATTTTTCATTATTTGGGTCGC
AGAGGGATTTTTGAGGAACGATGGTATCCGAAGAATGATATAAAAAAGCTTTCACAAATT
GATGAATTTATGCAATGGCATCACAATTCTTTGGCATTTAGTGCAGCATCGATTTTTTAT
AATGCATGGATAAAGCCTATTCGTGAAATTAATATGCTGGAACATGGAATGATGAGAAAT
GTTCGTGATCCTTTAAATTATGTTGACATTGATAATTCATTAGATTTGCTTGAAAATACA
TGGTTAAAAGAAAACAAGTTTATTGTGGGTGAGGAACCAACTTTTGCTGAAATTCTTGCC
TCATGCACTCTAATGCAAGTCATCGGTCTGCGACTTTTCAGACTTGACGAGCAGAAATAT
CAAAAAGTAACAAAATGGCTGAATGCAACGAAATCTTATTTTAATCCAGAATTCGATGAA
TTGCACAAATACATATATAAATATGGAGAGAAATTTAATGGAAAAAGTCCTTTTGAAAAT
TAA

>g13887.t1 Gene=g13887 Length=240
MAKSFKIFYDHFSPLSRSLLILFERNNVECEKISVALRKGEHLSEEFGDKINRFHKVPVI
HDSDGFRLAESIAIFHYLGRRGIFEERWYPKNDIKKLSQIDEFMQWHHNSLAFSAASIFY
NAWIKPIREINMLEHGMMRNVRDPLNYVDIDNSLDLLENTWLKENKFIVGEEPTFAEILA
SCTLMQVIGLRLFRLDEQKYQKVTKWLNATKSYFNPEFDELHKYIYKYGEKFNGKSPFEN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g13887.t1 Gene3D G3DSA:3.40.30.10 Glutaredoxin 1 81 0.0000000
6 g13887.t1 Gene3D G3DSA:1.20.1050.10 - 87 238 0.0000000
3 g13887.t1 PANTHER PTHR43917 - 5 235 0.0000000
2 g13887.t1 Pfam PF02798 Glutathione S-transferase, N-terminal domain 9 80 0.0000001
1 g13887.t1 Pfam PF00043 Glutathione S-transferase, C-terminal domain 152 208 0.0000046
9 g13887.t1 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile. 3 86 16.1880000
8 g13887.t1 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile. 93 238 10.9160000
10 g13887.t1 SFLD SFLDG00358 Main (cytGST) 6 211 0.0000000
11 g13887.t1 SFLD SFLDS00019 Glutathione Transferase (cytosolic) 6 211 0.0000000
4 g13887.t1 SUPERFAMILY SSF52833 Thioredoxin-like 6 92 0.0000000
5 g13887.t1 SUPERFAMILY SSF47616 GST C-terminal domain-like 86 221 0.0000000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006749 glutathione metabolic process BP
GO:0005515 protein binding MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values