Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Major facilitator superfamily domain-containing protein 8.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13914 g13914.t2 isoform g13914.t2 33373157 33374233
chr_1 g13914 g13914.t2 TSS g13914.t2 33373157 33373157
chr_1 g13914 g13914.t2 exon g13914.t2.exon1 33373157 33373308
chr_1 g13914 g13914.t2 exon g13914.t2.exon2 33373643 33373905
chr_1 g13914 g13914.t2 cds g13914.t2.CDS1 33373717 33373905
chr_1 g13914 g13914.t2 exon g13914.t2.exon3 33373986 33374233
chr_1 g13914 g13914.t2 cds g13914.t2.CDS2 33373986 33374231
chr_1 g13914 g13914.t2 TTS g13914.t2 NA NA

Sequences

>g13914.t2 Gene=g13914 Length=663
CAGTTTGCGAGGCACTTTTGTGTGAGGCAGAAGTACATTTTTTTCTCTCTTTACAAAATC
ACAATCATGAACTTTTTGATTTGTAAAAAGTAAAAATATTGTGTCGTTCACAATCGTGTA
ATTTTTTTTCTCAATACATTAAAATCCAACAGATTGTGCAAAATCATTTGCAAATTTGAG
TGAAAAAGAGAGAGAGAGAGAGAAGGAAACTTTATGCAATAATAATATGGAGAATAATGA
GCTGAAATCATCAGACTCTGCAGATGCAGAAGCAATAAATCGAGATCAAATACTTGAAAC
ACCATCGGAGCGTAGAATTCGCATCTTTTCTCTCAATATAATTTATTTTACAATGTTTTT
AATGACACTTGGTTTTTCAATAATATTAACTGCCATTTGGCCATATATTGATAAGTTAGA
TAGAGACGCTGGTAAAGTATTTATGGGTTGGATTGTTGCTGCTAATCCTCTAGGACAATT
TATTTTCTCTCCCGTATTTGGATGGTGGGCAAATAAATCAAAATCGATAAGAACGCCATT
TATCGTATCTATGATTATTTTTTGCGTCGCAAGTGCAGTCTATTCATGTCTGGACATTGT
TGAAAATAATGTAAAATATTGGATGCTTGTTTGTAGATTTTTTGTTGGCGTCTCATCGGC
AAA

>g13914.t2 Gene=g13914 Length=145
MENNELKSSDSADAEAINRDQILETPSERRIRIFSLNIIYFTMFLMTLGFSIILTAIWPY
IDKLDRDAGKVFMGWIVAANPLGQFIFSPVFGWWANKSKSIRTPFIVSMIIFCVASAVYS
CLDIVENNVKYWMLVCRFFVGVSSA

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g13914.t2 Gene3D G3DSA:1.20.1250.20 MFS general substrate transporter like domains 28 145 1.3E-15
2 g13914.t2 PANTHER PTHR23510:SF3 MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 8 24 144 8.4E-44
3 g13914.t2 PANTHER PTHR23510 INNER MEMBRANE TRANSPORT PROTEIN YAJR 24 144 8.4E-44
1 g13914.t2 Pfam PF07690 Major Facilitator Superfamily 39 144 1.2E-13
10 g13914.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 37 -
15 g13914.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 38 61 -
12 g13914.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 62 72 -
16 g13914.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 73 93 -
11 g13914.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 94 104 -
14 g13914.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 105 125 -
13 g13914.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 126 145 -
9 g13914.t2 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile. 35 145 12.207
7 g13914.t2 SUPERFAMILY SSF103473 MFS general substrate transporter 13 144 8.24E-17
4 g13914.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 39 61 -
6 g13914.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 71 93 -
5 g13914.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 105 127 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0055085 transmembrane transport BP
GO:0022857 transmembrane transporter activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values