Gene loci information

Transcript annotation

  • This transcript has been annotated as Major facilitator superfamily domain-containing protein 8.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13914 g13914.t3 isoform g13914.t3 33373157 33375461
chr_1 g13914 g13914.t3 TSS g13914.t3 33373157 33373157
chr_1 g13914 g13914.t3 exon g13914.t3.exon1 33373157 33373308
chr_1 g13914 g13914.t3 exon g13914.t3.exon2 33373643 33373905
chr_1 g13914 g13914.t3 cds g13914.t3.CDS1 33373717 33373905
chr_1 g13914 g13914.t3 exon g13914.t3.exon3 33373986 33374238
chr_1 g13914 g13914.t3 cds g13914.t3.CDS2 33373986 33374238
chr_1 g13914 g13914.t3 exon g13914.t3.exon4 33374341 33375461
chr_1 g13914 g13914.t3 cds g13914.t3.CDS3 33374341 33375461
chr_1 g13914 g13914.t3 TTS g13914.t3 NA NA

Sequences

>g13914.t3 Gene=g13914 Length=1789
CAGTTTGCGAGGCACTTTTGTGTGAGGCAGAAGTACATTTTTTTCTCTCTTTACAAAATC
ACAATCATGAACTTTTTGATTTGTAAAAAGTAAAAATATTGTGTCGTTCACAATCGTGTA
ATTTTTTTTCTCAATACATTAAAATCCAACAGATTGTGCAAAATCATTTGCAAATTTGAG
TGAAAAAGAGAGAGAGAGAGAGAAGGAAACTTTATGCAATAATAATATGGAGAATAATGA
GCTGAAATCATCAGACTCTGCAGATGCAGAAGCAATAAATCGAGATCAAATACTTGAAAC
ACCATCGGAGCGTAGAATTCGCATCTTTTCTCTCAATATAATTTATTTTACAATGTTTTT
AATGACACTTGGTTTTTCAATAATATTAACTGCCATTTGGCCATATATTGATAAGTTAGA
TAGAGACGCTGGTAAAGTATTTATGGGTTGGATTGTTGCTGCTAATCCTCTAGGACAATT
TATTTTCTCTCCCGTATTTGGATGGTGGGCAAATAAATCAAAATCGATAAGAACGCCATT
TATCGTATCTATGATTATTTTTTGCGTCGCAAGTGCAGTCTATTCATGTCTGGACATTGT
TGAAAATAATGTAAAATATTGGATGCTTGTTTGTAGATTTTTTGTTGGCGTCTCATCGGC
AAACATTGTTATTTGTAGAAGCTATGTGTCAGCTGCTACTAAACTTAAGGAAAGAACAAA
GGCTGTGTCTTTATTAACACTTGCCCAAACACTTGGATTTATATTTGGTCCACTTTTTCA
AGCGGCATTTACTTCATTAGGAAGTGAAGGATATAAAATGTACTTTAATTTACCACTATC
GATGTATACAGCACCAGGATGGCTCAATGTTTTCCTTGGCTTATTCAATTTGATACTATT
TCATCCTAAAATTTTCCAAGACAAAAGAGTCGCTGCACGTGAACAGATGTTAATTCAGGG
AAAAGCTTCAGAAAAAGAAGCATGGAAAAATATTAAGCCTGATTACTTTGTTTCATGGGC
ACTCATATTTTCATTATTTGTATTTGTTTTTAATTTCGTATTGCTCGAAAGTATGGGAAC
AGCTTTAACAATGGAACAATTTGCATGGACAAAGAAACAAGCAATTGAATATATGGCGTA
TATTATGGGTATTGGCGGTTTGATAGCATGTGGTACATTTGTAGCAATATCACCGCTCTG
TAAGAAATTTAGAGAAAATGATGTGCTGATATATGGTGGCTTTTTTGTTATGATATTATC
GCGTATAACTCATATTCCCTACGGAAGTACAATACCAAAATTAGCTTTACCCAAAGAATA
CACATTCGAAAATGGCACACATGTGATATTTGAAGAAGATGATCCTCACATTCTCGGTTG
TCCGGCATATGAGCAAAAGTGGTGTGAAACAACTCCAGCATTAGGATTCTATGAATTTAT
TTTAGGATACATTCTCACAAGTTTTGGCTATCCTATTGGCCTAACATTAATTCAAACAAT
TTTCTCAAAAGTTCTTGGACCACGACCGCAAGGTGTTTGGATGAGTTTGATGACAAATGC
CGGTTGTCTATCAAGAATTTTTGGACCAATTTGTGTATCAATGCTTTATGCTCGCTTTGG
CACAAGAATTACAATGTTATTTACTCTAATAATTATGATAATTCCAATGGTTTGGCTTCT
TTTCTTAAAAGACCGCATGCTGATTGAAGATTTTAAAGATAAAACAATTGAAATGGAAGA
AAGAAATGGATTAAAACCAAATATACAAATTAATGATAACGATAGATGA

>g13914.t3 Gene=g13914 Length=520
MENNELKSSDSADAEAINRDQILETPSERRIRIFSLNIIYFTMFLMTLGFSIILTAIWPY
IDKLDRDAGKVFMGWIVAANPLGQFIFSPVFGWWANKSKSIRTPFIVSMIIFCVASAVYS
CLDIVENNVKYWMLVCRFFVGVSSANIVICRSYVSAATKLKERTKAVSLLTLAQTLGFIF
GPLFQAAFTSLGSEGYKMYFNLPLSMYTAPGWLNVFLGLFNLILFHPKIFQDKRVAAREQ
MLIQGKASEKEAWKNIKPDYFVSWALIFSLFVFVFNFVLLESMGTALTMEQFAWTKKQAI
EYMAYIMGIGGLIACGTFVAISPLCKKFRENDVLIYGGFFVMILSRITHIPYGSTIPKLA
LPKEYTFENGTHVIFEEDDPHILGCPAYEQKWCETTPALGFYEFILGYILTSFGYPIGLT
LIQTIFSKVLGPRPQGVWMSLMTNAGCLSRIFGPICVSMLYARFGTRITMLFTLIIMIIP
MVWLLFLKDRMLIEDFKDKTIEMEERNGLKPNIQINDNDR

Protein features from InterProScan

Transcript Database ID Name Start End E.value
43 g13914.t3 CDD cd17326 MFS_MFSD8 38 491 2.96508E-120
2 g13914.t3 PANTHER PTHR23510:SF3 MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 8 25 491 5.1E-148
3 g13914.t3 PANTHER PTHR23510 INNER MEMBRANE TRANSPORT PROTEIN YAJR 25 491 5.1E-148
1 g13914.t3 Pfam PF07690 Major Facilitator Superfamily 39 345 4.9E-34
19 g13914.t3 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 37 -
33 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 38 61 -
27 g13914.t3 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 62 72 -
42 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 73 93 -
23 g13914.t3 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 94 104 -
32 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 105 125 -
30 g13914.t3 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 126 130 -
34 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 131 154 -
20 g13914.t3 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 155 165 -
39 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 166 184 -
25 g13914.t3 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 185 203 -
36 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 204 225 -
22 g13914.t3 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 226 259 -
35 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 260 279 -
29 g13914.t3 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 280 298 -
31 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 299 321 -
24 g13914.t3 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 322 332 -
40 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 333 352 -
28 g13914.t3 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 353 404 -
41 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 405 426 -
18 g13914.t3 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 427 437 -
37 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 438 462 -
26 g13914.t3 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 463 467 -
38 g13914.t3 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 468 487 -
21 g13914.t3 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 488 520 -
17 g13914.t3 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile. 35 492 11.973
16 g13914.t3 SUPERFAMILY SSF103473 MFS general substrate transporter 35 491 5.89E-44
10 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 39 61 -
7 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 71 93 -
8 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 100 122 -
5 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 132 154 -
14 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 166 188 -
15 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 203 225 -
9 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 260 279 -
4 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 299 321 -
6 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 333 350 -
12 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 404 426 -
11 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 447 464 -
13 g13914.t3 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 468 487 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0055085 transmembrane transport BP
GO:0022857 transmembrane transporter activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values