Gene loci information

Transcript annotation

  • This transcript has been annotated as Major facilitator superfamily domain-containing protein 8.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_1 g13914 g13914.t6 TSS g13914.t6 33373157 33373157
chr_1 g13914 g13914.t6 isoform g13914.t6 33373984 33375461
chr_1 g13914 g13914.t6 exon g13914.t6.exon1 33373984 33374238
chr_1 g13914 g13914.t6 cds g13914.t6.CDS1 33374013 33374238
chr_1 g13914 g13914.t6 exon g13914.t6.exon2 33374341 33375461
chr_1 g13914 g13914.t6 cds g13914.t6.CDS2 33374341 33375461
chr_1 g13914 g13914.t6 TTS g13914.t6 NA NA

Sequences

>g13914.t6 Gene=g13914 Length=1376
AGTTAGATAGAGACGCTGGTAAAGTATTTATGGGTTGGATTGTTGCTGCTAATCCTCTAG
GACAATTTATTTTCTCTCCCGTATTTGGATGGTGGGCAAATAAATCAAAATCGATAAGAA
CGCCATTTATCGTATCTATGATTATTTTTTGCGTCGCAAGTGCAGTCTATTCATGTCTGG
ACATTGTTGAAAATAATGTAAAATATTGGATGCTTGTTTGTAGATTTTTTGTTGGCGTCT
CATCGGCAAACATTGTTATTTGTAGAAGCTATGTGTCAGCTGCTACTAAACTTAAGGAAA
GAACAAAGGCTGTGTCTTTATTAACACTTGCCCAAACACTTGGATTTATATTTGGTCCAC
TTTTTCAAGCGGCATTTACTTCATTAGGAAGTGAAGGATATAAAATGTACTTTAATTTAC
CACTATCGATGTATACAGCACCAGGATGGCTCAATGTTTTCCTTGGCTTATTCAATTTGA
TACTATTTCATCCTAAAATTTTCCAAGACAAAAGAGTCGCTGCACGTGAACAGATGTTAA
TTCAGGGAAAAGCTTCAGAAAAAGAAGCATGGAAAAATATTAAGCCTGATTACTTTGTTT
CATGGGCACTCATATTTTCATTATTTGTATTTGTTTTTAATTTCGTATTGCTCGAAAGTA
TGGGAACAGCTTTAACAATGGAACAATTTGCATGGACAAAGAAACAAGCAATTGAATATA
TGGCGTATATTATGGGTATTGGCGGTTTGATAGCATGTGGTACATTTGTAGCAATATCAC
CGCTCTGTAAGAAATTTAGAGAAAATGATGTGCTGATATATGGTGGCTTTTTTGTTATGA
TATTATCGCGTATAACTCATATTCCCTACGGAAGTACAATACCAAAATTAGCTTTACCCA
AAGAATACACATTCGAAAATGGCACACATGTGATATTTGAAGAAGATGATCCTCACATTC
TCGGTTGTCCGGCATATGAGCAAAAGTGGTGTGAAACAACTCCAGCATTAGGATTCTATG
AATTTATTTTAGGATACATTCTCACAAGTTTTGGCTATCCTATTGGCCTAACATTAATTC
AAACAATTTTCTCAAAAGTTCTTGGACCACGACCGCAAGGTGTTTGGATGAGTTTGATGA
CAAATGCCGGTTGTCTATCAAGAATTTTTGGACCAATTTGTGTATCAATGCTTTATGCTC
GCTTTGGCACAAGAATTACAATGTTATTTACTCTAATAATTATGATAATTCCAATGGTTT
GGCTTCTTTTCTTAAAAGACCGCATGCTGATTGAAGATTTTAAAGATAAAACAATTGAAA
TGGAAGAAAGAAATGGATTAAAACCAAATATACAAATTAATGATAACGATAGATGA

>g13914.t6 Gene=g13914 Length=448
MGWIVAANPLGQFIFSPVFGWWANKSKSIRTPFIVSMIIFCVASAVYSCLDIVENNVKYW
MLVCRFFVGVSSANIVICRSYVSAATKLKERTKAVSLLTLAQTLGFIFGPLFQAAFTSLG
SEGYKMYFNLPLSMYTAPGWLNVFLGLFNLILFHPKIFQDKRVAAREQMLIQGKASEKEA
WKNIKPDYFVSWALIFSLFVFVFNFVLLESMGTALTMEQFAWTKKQAIEYMAYIMGIGGL
IACGTFVAISPLCKKFRENDVLIYGGFFVMILSRITHIPYGSTIPKLALPKEYTFENGTH
VIFEEDDPHILGCPAYEQKWCETTPALGFYEFILGYILTSFGYPIGLTLIQTIFSKVLGP
RPQGVWMSLMTNAGCLSRIFGPICVSMLYARFGTRITMLFTLIIMIIPMVWLLFLKDRML
IEDFKDKTIEMEERNGLKPNIQINDNDR

Protein features from InterProScan

Transcript Database ID Name Start End E.value
39 g13914.t6 CDD cd17326 MFS_MFSD8 1 419 3.88137E-103
3 g13914.t6 PANTHER PTHR23510:SF3 MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 8 1 419 4.1E-127
4 g13914.t6 PANTHER PTHR23510 INNER MEMBRANE TRANSPORT PROTEIN YAJR 1 419 4.1E-127
2 g13914.t6 Pfam PF07690 Major Facilitator Superfamily 1 273 1.8E-31
1 g13914.t6 Pfam PF07690 Major Facilitator Superfamily 329 415 1.8E-8
23 g13914.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 32 -
36 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 33 53 -
24 g13914.t6 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 54 58 -
29 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 59 82 -
20 g13914.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 83 93 -
33 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 94 112 -
26 g13914.t6 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 113 131 -
35 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 132 153 -
21 g13914.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 154 187 -
32 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 188 207 -
25 g13914.t6 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 208 226 -
30 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 227 249 -
19 g13914.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 250 260 -
38 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 261 280 -
28 g13914.t6 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 281 332 -
31 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 333 354 -
18 g13914.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 355 365 -
34 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 366 390 -
27 g13914.t6 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 391 395 -
37 g13914.t6 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 396 415 -
22 g13914.t6 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 416 448 -
17 g13914.t6 ProSiteProfiles PS50850 Major facilitator superfamily (MFS) profile. 1 420 10.888
16 g13914.t6 SUPERFAMILY SSF103473 MFS general substrate transporter 1 419 2.62E-37
8 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 4 23 -
5 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 32 54 -
11 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 59 81 -
7 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 94 116 -
13 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 131 153 -
10 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 188 207 -
6 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 227 249 -
9 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 261 278 -
12 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 332 354 -
15 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 375 392 -
14 g13914.t6 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 396 415 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0055085 transmembrane transport BP
GO:0022857 transmembrane transporter activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values