General information for g14064.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as N-acetylglucosamine-6-sulfatase.
Parent gene
- The parent gene g14064 is located on chr_1: 34460141..34461695 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14064 | g14064.t1 | TTS | g14064.t1 | 34459963 | 34459963 |
| chr_1 | g14064 | g14064.t1 | isoform | g14064.t1 | 34460141 | 34461695 |
| chr_1 | g14064 | g14064.t1 | exon | g14064.t1.exon1 | 34460141 | 34461466 |
| chr_1 | g14064 | g14064.t1 | cds | g14064.t1.CDS1 | 34460141 | 34461466 |
| chr_1 | g14064 | g14064.t1 | exon | g14064.t1.exon2 | 34461525 | 34461695 |
| chr_1 | g14064 | g14064.t1 | cds | g14064.t1.CDS2 | 34461525 | 34461695 |
| chr_1 | g14064 | g14064.t1 | TSS | g14064.t1 | 34461760 | 34461760 |
Sequences
>g14064.t1 Gene=g14064 Length=1497
ATGAATCTATTTATTTTATTACTTTTATTTCTATCTTTAACAAGTGCTCTATCAAAACCG
AATTTTGTGATAATATTAACTGATGATCAAGATTTATTATTAGACTCTCTTAAACCATTA
CAAAAAATTGAAAAATATTTGATAAATAATGGAGTGATATTTTTAAATGCTTTTACATCA
TCACCTATTTGCTGCCCAAGTCGATCAAGTTTTTTAACTGGAAATTTTGCACACAATACA
AATACTTTCAACAATTCATTGAACGGTGGATGTTATTCAAATGAATGGATTGAAAATCAT
GAACTAAGAACATTTCCTGCTATTTTAAATAATCATGGTTATCGAACATTCTATGCTGGA
AAATATTTAAATCAATATTATTCTAATCTCATTCCACAAGGGTATGATCAATTTTATGGA
CTACATGGAAACAGTAAATACTTCAATTATACACTAAATGAAAATGGAAATATTATCAAG
TACGGTGATAATCAAGAAGATTACTACACAAATGTTATCAAGAGTCAGATTCTAAATTTT
CTAAGTAATCAAACGAAAGATGAACCGTTTATTGCTATTGTATCAACACCGTCATGTCAT
GCACCATTCACACCTGAGGAAAAATTTAATAATTCATTATCAAATTTTACTGCACCACGC
ACGAGTAATTTTAATGTTGGTTCAAGAGGTTTAGACAAACACTGGCTTATGACAATGAAA
CCTAAGGAACTTTCTCAAGAAACAATTGCAAAAATTGATGAATTTTATCGTATGAGATTA
GAGAGTCTTTTATCAGTCGATGACATGGTTGAAGAGATAATAGAAAAATTATCTGAACAA
AACATTTTAGATGAAACTTTTATTATATTTACATCAGATAACGGGTATCATTTAGGAAAT
TGGGCAATGCCGTGGGACAAAAGATTGCCATATGAAACTGACATAAAAATTCCACTAATT
ATTAGAGGACCGAACGTTCCAGTGAAAAAAGTAGTTCAATCTCCTGTTGCTCTTATTGAT
TTAGCTCCAACAATTCTTAATTGGGCTGAAATAAATTATAAGAGTGAGAATTTTGATGGA
CAATCTTTTGATAAGTTGATTAATGAAAAATTTTTCAATGAGAATTTTATAAATGAGAGG
CAAATTTTAATAGAGCATTGGGGAGAAGGAAATGTTGAAACATTCAATCCTGAATGTCCA
TGGAAGAAAAGCCAAAGATTAAGTGAATGCCAGATTGAAGCAGAATGTAAATGTCAAGAT
TCTTGGAATAATACTTATGTATGTGTCAGACATTTGTCAAAAGATATAAATTTCTTGTAT
TGCACTTTCCATGATCGAGAACATTTCAATGAAGCTTATGATTTAGGTGAAGATGAATTT
CAATTGAACAATGTTGCATACGATATTCTACCATCAATTCAAGCTCGCTATCAAATTATT
GTTGAAAATTTAAGAAATTGTCTTGGTGAAGATTGTAAAATTGTTAAAAATGTTTGA
>g14064.t1 Gene=g14064 Length=498
MNLFILLLLFLSLTSALSKPNFVIILTDDQDLLLDSLKPLQKIEKYLINNGVIFLNAFTS
SPICCPSRSSFLTGNFAHNTNTFNNSLNGGCYSNEWIENHELRTFPAILNNHGYRTFYAG
KYLNQYYSNLIPQGYDQFYGLHGNSKYFNYTLNENGNIIKYGDNQEDYYTNVIKSQILNF
LSNQTKDEPFIAIVSTPSCHAPFTPEEKFNNSLSNFTAPRTSNFNVGSRGLDKHWLMTMK
PKELSQETIAKIDEFYRMRLESLLSVDDMVEEIIEKLSEQNILDETFIIFTSDNGYHLGN
WAMPWDKRLPYETDIKIPLIIRGPNVPVKKVVQSPVALIDLAPTILNWAEINYKSENFDG
QSFDKLINEKFFNENFINERQILIEHWGEGNVETFNPECPWKKSQRLSECQIEAECKCQD
SWNNTYVCVRHLSKDINFLYCTFHDREHFNEAYDLGEDEFQLNNVAYDILPSIQARYQII
VENLRNCLGEDCKIVKNV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g14064.t1 | CDD | cd16147 | G6S | 19 | 469 | 1.67309E-162 |
| 7 | g14064.t1 | Coils | Coil | Coil | 495 | 498 | - |
| 6 | g14064.t1 | Gene3D | G3DSA:3.40.720.10 | Alkaline Phosphatase | 19 | 465 | 2.0E-79 |
| 2 | g14064.t1 | PANTHER | PTHR43108 | N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER | 6 | 493 | 2.6E-175 |
| 3 | g14064.t1 | PANTHER | PTHR43108:SF13 | SD21168P | 6 | 493 | 2.6E-175 |
| 13 | g14064.t1 | PIRSF | PIRSF036666 | G6S | 1 | 497 | 1.5E-170 |
| 1 | g14064.t1 | Pfam | PF00884 | Sulfatase | 20 | 350 | 2.1E-60 |
| 9 | g14064.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 10 | g14064.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 11 | g14064.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 13 | - |
| 12 | g14064.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 8 | g14064.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 498 | - |
| 15 | g14064.t1 | ProSitePatterns | PS00523 | Sulfatases signature 1. | 62 | 74 | - |
| 16 | g14064.t1 | ProSitePatterns | PS00149 | Sulfatases signature 2. | 113 | 123 | - |
| 4 | g14064.t1 | SUPERFAMILY | SSF53649 | Alkaline phosphatase-like | 18 | 492 | 3.63E-88 |
| 5 | g14064.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008484 | sulfuric ester hydrolase activity | MF |
| GO:0008449 | N-acetylglucosamine-6-sulfatase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0030203 | glycosaminoglycan metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01137 (N-acetylglucosamine-6-sulfatase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
