General information for g14087.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein kinase C, brain isozyme.
Parent gene
- The parent gene g14087 is located on chr_1: 34565895..34566329 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14087 | g14087.t1 | TSS | g14087.t1 | 34527422 | 34527422 |
| chr_1 | g14087 | g14087.t1 | isoform | g14087.t1 | 34528281 | 34566329 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon1 | 34528281 | 34528462 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS1 | 34528281 | 34528462 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon2 | 34553524 | 34553555 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS2 | 34553524 | 34553555 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon3 | 34553618 | 34553700 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS3 | 34553618 | 34553700 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon4 | 34557130 | 34557204 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS4 | 34557130 | 34557204 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon5 | 34557294 | 34557330 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS5 | 34557294 | 34557330 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon6 | 34562400 | 34562528 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS6 | 34562400 | 34562528 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon7 | 34562639 | 34562648 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS7 | 34562639 | 34562648 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon8 | 34562716 | 34562859 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS8 | 34562716 | 34562859 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon9 | 34563005 | 34563047 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS9 | 34563005 | 34563047 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon10 | 34563333 | 34563371 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS10 | 34563333 | 34563371 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon11 | 34563472 | 34563948 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS11 | 34563472 | 34563948 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon12 | 34564042 | 34564109 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS12 | 34564042 | 34564109 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon13 | 34565254 | 34565369 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS13 | 34565254 | 34565369 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon14 | 34565479 | 34565562 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS14 | 34565479 | 34565562 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon15 | 34565630 | 34565820 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS15 | 34565630 | 34565820 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon16 | 34565895 | 34566035 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS16 | 34565895 | 34566035 |
| chr_1 | g14087 | g14087.t1 | exon | g14087.t1.exon17 | 34566177 | 34566329 |
| chr_1 | g14087 | g14087.t1 | cds | g14087.t1.CDS17 | 34566177 | 34566329 |
| chr_1 | g14087 | g14087.t1 | TTS | g14087.t1 | 34566519 | 34566519 |
Sequences
>g14087.t1 Gene=g14087 Length=2004
ATGGCAGAAGATGAAAATAATCAAGAGAATGATCAGGGAGGTGACAACAAGTTGGCACTT
CGTAATCGTGGACGTAAGGGTGCATTGAAGAAGAAAAATGTGTATAATGTTAAGGATCAT
AGATTCATTCCGCGCTTTTTCAAGCAACCAACTTTTTGCTCTCATTGCAAAGACTTCATT
TGGGGTTTTGGAAAACAGGGCTTTCAGTGTCAAGTTTGTTCGTTCGTCGTTCACAAGCGA
TGCCATGAATATGTAACTTTTAAGTGTCCAGGTGCTGACAAAGGCGCTGACTCAGATGAC
ACACGAACCAAACACAACTTTGCACCCCAAACTTACTCAAGTCCGACATTTTGTGACCAT
TGTGGCTCTTTGTTGTATGGGCTACTGCATCAGGGTTTAAGATGTCAAGTATGTGATATG
AATGTGCATAAAAGATGTGAAGAAAGCGTACCAAACTTGTGTGGATGCGATCACACTGAA
CGACGCGGTAGAATTAATCTAAACATCACTTGCGTTGCCAATAAATTATCGATAGAAGTT
AAAGAAGGACGAAATCTTATACCAATGGATCCAAATGGTCTTTCAGATCCATATGTTAAG
ATTAAGTTGATTCCAGATTCTGACTCTGTAAAAAAGAAAACAAAAACCATAAAAGCAACA
TTAAATCCTGTATGGAATGAGACACTCGTTTTCGATTTAAAACAAGAGGACAAAGATCGA
CGCATTTTGATAGAGGTTTGGGATTGGGATAGAACAAGTCGCAATGACTTTATGGGCTCG
TTATCGTTTGGCATCAGCGAGATTATAAAGATGCCTGCTAAAGGATGGTTTAAATTGTTA
ACAGCCGAGGAAGGTGAATTTTATAATGTGCCCGTTCCAGATGATAATGCCGATTTGGCT
CAATTAAAGATGCAAATGCGCAAGACTTCCATTACAAAAAAAGCACCGGTTATCACGGAT
AAGGACGTGCCACATAATATGGGTAAAAAGGATGTCATAAGAGCGACAGACTTTAATTTC
CTCATGGTGCTCGGAAAGGGCTCATTTGGAAAGGTAATGCTGGCCGAACGAAAAGGCACG
GATGAATTGTATGCCATCAAAATCCTTAAGAAGGACATCATCATTCAAGACGATGATGTT
GAATGTACTATGGTAGAGAAACGTGTACTCGCGCAATCAGGCAAGCCTCCCTTCCTCGTG
CAACTTCACTCTTGCTTCCAAACAATGGATCGCTTGTATTTTGTCATGGAGTACGTAAAT
GGCGGTGATTTAATGTTTCAAATTCAACAGTGTGGCAAATTCAAGGAACCTGTTGCTGTA
TTTTATGCGGCCGAAATTGCCATTGGTTTGTTTTTCTTACACTCGTGTGGAATCATTTAT
CGTGATTTGAAATTGGACAACGTGCTGCTGGATCAAGATGGCCACATTAAAATAGCTGAT
TTCGGTATGTGTAAAGAGAATATTTCGAACGATAAAACAACAAAAACATTTTGTGGAACA
CCCGATTACATAGCCCCAGAGATCATTTTGTATCAGCCGTATGGTAAAAGTGTAGATTGG
TGGGCTTATGGTGTACTTTTGTATGAGATGCTTGTTGGTCAGCCGCCGTTCGATGGCGAG
GATGAGGAGGAGCTTTTTGCAGCTATCACAGATCACAATGTTTCATATCCCAAGGGCTTG
AGCAAAGAAGCGAAGGACGTTTGTAAAGGATTTCTAACAAAGAATCCACAAAAAAGATTG
GGTTGTGGTGCAAATGGCGAAGACGATGTTCGAAATCACAGTTTCTTCCGTCGTATCGAT
TGGGAGAAAATTGAGAACCGTGAAGTTCAGCCACCATTTAAACCTAAAATTAAACATCGC
AAAGATGTATCAAATTTTGATCGCCAATTTACATCAGAAAAGACAGATTTAACGCCCACT
GACAAACTATTTATGATGAATCTTGATCAAACTGAATTCACTGGCTTTTCTTATCTAAAT
CCAGAATTTGTTCAACATATTTAA
>g14087.t1 Gene=g14087 Length=667
MAEDENNQENDQGGDNKLALRNRGRKGALKKKNVYNVKDHRFIPRFFKQPTFCSHCKDFI
WGFGKQGFQCQVCSFVVHKRCHEYVTFKCPGADKGADSDDTRTKHNFAPQTYSSPTFCDH
CGSLLYGLLHQGLRCQVCDMNVHKRCEESVPNLCGCDHTERRGRINLNITCVANKLSIEV
KEGRNLIPMDPNGLSDPYVKIKLIPDSDSVKKKTKTIKATLNPVWNETLVFDLKQEDKDR
RILIEVWDWDRTSRNDFMGSLSFGISEIIKMPAKGWFKLLTAEEGEFYNVPVPDDNADLA
QLKMQMRKTSITKKAPVITDKDVPHNMGKKDVIRATDFNFLMVLGKGSFGKVMLAERKGT
DELYAIKILKKDIIIQDDDVECTMVEKRVLAQSGKPPFLVQLHSCFQTMDRLYFVMEYVN
GGDLMFQIQQCGKFKEPVAVFYAAEIAIGLFFLHSCGIIYRDLKLDNVLLDQDGHIKIAD
FGMCKENISNDKTTKTFCGTPDYIAPEIILYQPYGKSVDWWAYGVLLYEMLVGQPPFDGE
DEEELFAAITDHNVSYPKGLSKEAKDVCKGFLTKNPQKRLGCGANGEDDVRNHSFFRRID
WEKIENREVQPPFKPKIKHRKDVSNFDRQFTSEKTDLTPTDKLFMMNLDQTEFTGFSYLN
PEFVQHI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 30 | g14087.t1 | CDD | cd00029 | C1 | 40 | 89 | 1.14679E-18 |
| 29 | g14087.t1 | CDD | cd00029 | C1 | 105 | 154 | 3.4112E-17 |
| 31 | g14087.t1 | CDD | cd04026 | C2_PKC_alpha_gamma | 162 | 291 | 2.0285E-76 |
| 28 | g14087.t1 | CDD | cd05587 | STKc_cPKC | 341 | 660 | 0.0 |
| 21 | g14087.t1 | Gene3D | G3DSA:3.30.60.20 | - | 24 | 93 | 1.3E-25 |
| 20 | g14087.t1 | Gene3D | G3DSA:3.30.60.20 | - | 97 | 154 | 1.6E-24 |
| 23 | g14087.t1 | Gene3D | G3DSA:2.60.40.150 | - | 159 | 294 | 6.5E-44 |
| 19 | g14087.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 336 | 653 | 3.5E-143 |
| 22 | g14087.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 422 | 627 | 3.5E-143 |
| 40 | g14087.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 23 | - |
| 6 | g14087.t1 | PANTHER | PTHR24356 | SERINE/THREONINE-PROTEIN KINASE | 16 | 665 | 0.0 |
| 7 | g14087.t1 | PANTHER | PTHR24356:SF193 | PROTEIN KINASE C ALPHA TYPE | 16 | 665 | 0.0 |
| 27 | g14087.t1 | PIRSF | PIRSF000550 | PKC_alpha | 1 | 667 | 0.0 |
| 12 | g14087.t1 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 37 | 51 | 1.3E-14 |
| 11 | g14087.t1 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 53 | 62 | 1.3E-14 |
| 13 | g14087.t1 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 66 | 77 | 1.3E-14 |
| 14 | g14087.t1 | PRINTS | PR00008 | Diacylglycerol/phorbol-ester binding signature | 143 | 155 | 1.3E-14 |
| 10 | g14087.t1 | PRINTS | PR00360 | C2 domain signature | 191 | 203 | 1.2E-10 |
| 9 | g14087.t1 | PRINTS | PR00360 | C2 domain signature | 219 | 232 | 1.2E-10 |
| 8 | g14087.t1 | PRINTS | PR00360 | C2 domain signature | 242 | 250 | 1.2E-10 |
| 4 | g14087.t1 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 40 | 91 | 2.2E-18 |
| 5 | g14087.t1 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 105 | 157 | 3.0E-17 |
| 2 | g14087.t1 | Pfam | PF00168 | C2 domain | 175 | 279 | 3.4E-27 |
| 3 | g14087.t1 | Pfam | PF00069 | Protein kinase domain | 340 | 582 | 2.5E-66 |
| 1 | g14087.t1 | Pfam | PF00433 | Protein kinase C terminal domain | 617 | 658 | 1.8E-6 |
| 25 | g14087.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 438 | - |
| 26 | g14087.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 439 | 460 | - |
| 24 | g14087.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 461 | 667 | - |
| 38 | g14087.t1 | ProSitePatterns | PS00479 | Zinc finger phorbol-ester/DAG-type signature. | 40 | 89 | - |
| 39 | g14087.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 344 | 367 | - |
| 37 | g14087.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 458 | 470 | - |
| 44 | g14087.t1 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 39 | 89 | 16.604 |
| 43 | g14087.t1 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 104 | 154 | 16.236 |
| 41 | g14087.t1 | ProSiteProfiles | PS50004 | C2 domain profile. | 172 | 262 | 21.986 |
| 45 | g14087.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 338 | 596 | 49.092 |
| 42 | g14087.t1 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 597 | 667 | 18.703 |
| 36 | g14087.t1 | SMART | SM00109 | c1_12 | 40 | 89 | 5.2E-15 |
| 35 | g14087.t1 | SMART | SM00109 | c1_12 | 105 | 154 | 8.6E-18 |
| 32 | g14087.t1 | SMART | SM00239 | C2_3c | 175 | 277 | 2.2E-25 |
| 34 | g14087.t1 | SMART | SM00220 | serkin_6 | 338 | 596 | 1.5E-103 |
| 33 | g14087.t1 | SMART | SM00133 | pkinase_C_6 | 597 | 660 | 1.6E-23 |
| 16 | g14087.t1 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 28 | 90 | 9.72E-21 |
| 15 | g14087.t1 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 102 | 157 | 5.68E-19 |
| 18 | g14087.t1 | SUPERFAMILY | SSF49562 | C2 domain (Calcium/lipid-binding domain, CaLB) | 172 | 283 | 4.98E-34 |
| 17 | g14087.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 336 | 662 | 2.16E-96 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0008270 | zinc ion binding | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0035556 | intracellular signal transduction | BP |
| GO:0004697 | protein kinase C activity | MF |
KEGG
Orthology
- This transcript has been annotated with K02677 (classical protein kinase C alpha type)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
