General information for g14177.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as DNA polymerase epsilon catalytic subunit 1.
Parent gene
- The parent gene g14177 is located on chr_1: 35244564..35247204 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_1 | g14177 | g14177.t5 | TTS | g14177.t5 | 35239495 | 35239495 |
| chr_1 | g14177 | g14177.t5 | isoform | g14177.t5 | 35240256 | 35241426 |
| chr_1 | g14177 | g14177.t5 | exon | g14177.t5.exon1 | 35240256 | 35240265 |
| chr_1 | g14177 | g14177.t5 | exon | g14177.t5.exon2 | 35240373 | 35241169 |
| chr_1 | g14177 | g14177.t5 | cds | g14177.t5.CDS1 | 35240399 | 35241169 |
| chr_1 | g14177 | g14177.t5 | exon | g14177.t5.exon3 | 35241230 | 35241426 |
| chr_1 | g14177 | g14177.t5 | cds | g14177.t5.CDS2 | 35241230 | 35241298 |
| chr_1 | g14177 | g14177.t5 | TSS | g14177.t5 | NA | NA |
Sequences
>g14177.t5 Gene=g14177 Length=1004
ACAGTGGCAAAAAGAGTGTGGTTGATGGAATAAGTTATGCTGATTATGTTTGTCAAAGTA
TAAGAAACAAAGAACTATTTCATTCAGTTCATTTATCATATCAAAAATGTTGGAATTTTC
TATTATGGATGGATGATTACAATTTTTCTGGATTGAGGGGAAAAGTGCCACGAGAATTAG
TTGGTGAGAGTGAACAAATTGATGCAGATGATGAAGAACTTGCACTTGAAATGAATTGGA
ATATTTGTGAAGATTTACCTGAAGAAGGAAAGTGTCGCGATAAATTGGAAAATTTTCTTA
CTTTGTTCATTGAACAACTCGCTGATGGCATTGAATCTTCAAAAGCATTGAAAACTTTAT
CTCATCATGCATACGATATTGTAACGAAATTATATCAGAATTTTGGAAAGAATCAAGAAG
GACCAGCAACAGAATTCATCAAAGTGCTTTGTAAAATTCTTGAAATTGATACAACAGTGA
CTGATGAACTTGATTCATTAAGAAAGAACATGTTAAGACTTGTTGGATTAGGAGAATTTT
CAGACAAAGCCGTTTGGAAAGAGAAAGGAAAATCTTACATTTTAACTGAAATGATTTGTC
AAGCTTGTAATCACTGTCGAGATGTTGATTTGATGAAGGATAAGCATCGTGCTATGAAAA
ATGGTTTACCTGTATGGTTGTGTGCTCAATGCTTTGTTAATTATGACACAGAAGATGTTG
AACAACGTTTAATTGATGCATTGAATCGTAAACTCATGTCTTATACTCTTCAAGATCTTC
AATGTACTCGCTGTAAAGAAATCAAACAAGATAACATCATGCAATATTGTCATTGTGCAG
GAAATTACACAAATCTTATACAATCAAGTGAAATTGAAAGTTTAATAACTACTTTTAATC
TTGTTGCTGATAATTATTCGATGATTTTACTAAAAGAATATACTGATACGCTTCTAAATA
ATATTTAAGCATGAATAAATTTGTATAAAATTAATGGTTTAACA
>g14177.t5 Gene=g14177 Length=279
MDDYNFSGLRGKVPRELVGESEQIDADDEELALEMNWNICEDLPEEGKCRDKLENFLTLF
IEQLADGIESSKALKTLSHHAYDIVTKLYQNFGKNQEGPATEFIKVLCKILEIDTTVTDE
LDSLRKNMLRLVGLGEFSDKAVWKEKGKSYILTEMICQACNHCRDVDLMKDKHRAMKNGL
PVWLCAQCFVNYDTEDVEQRLIDALNRKLMSYTLQDLQCTRCKEIKQDNIMQYCHCAGNY
TNLIQSSEIESLITTFNLVADNYSMILLKEYTDTLLNNI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value |
|---|---|---|---|---|---|---|
| g14177.t5 | PANTHER | PTHR10670 | DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A | 1 | 276 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003887 | DNA-directed DNA polymerase activity | MF |
| GO:0003677 | DNA binding | MF |
| GO:0006281 | DNA repair | BP |
| GO:0006260 | DNA replication | BP |
| GO:0008622 | epsilon DNA polymerase complex | CC |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
