General information for g14389.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as GATOR complex protein Iml1.
Parent gene
- The parent gene g14389 is located on chr_4: 235182..236686 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14389 | g14389.t1 | TSS | g14389.t1 | 228204 | 228204 |
| chr_4 | g14389 | g14389.t1 | isoform | g14389.t1 | 228456 | 236686 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon1 | 228456 | 228546 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS1 | 228456 | 228546 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon2 | 228838 | 229057 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS2 | 228838 | 229057 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon3 | 229478 | 229596 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS3 | 229478 | 229596 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon4 | 230085 | 230740 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS4 | 230085 | 230740 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon5 | 230799 | 230846 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS5 | 230799 | 230846 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon6 | 230909 | 231839 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS6 | 230909 | 231839 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon7 | 231894 | 232022 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS7 | 231894 | 232022 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon8 | 232641 | 232658 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS8 | 232641 | 232658 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon9 | 233160 | 233377 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS9 | 233160 | 233377 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon10 | 233434 | 233820 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS10 | 233434 | 233820 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon11 | 233886 | 234086 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS11 | 233886 | 234086 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon12 | 234336 | 234425 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS12 | 234336 | 234425 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon13 | 234716 | 234783 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS13 | 234716 | 234783 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon14 | 235006 | 235128 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS14 | 235006 | 235128 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon15 | 235182 | 235827 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS15 | 235182 | 235827 |
| chr_4 | g14389 | g14389.t1 | exon | g14389.t1.exon16 | 235895 | 236686 |
| chr_4 | g14389 | g14389.t1 | cds | g14389.t1.CDS16 | 235895 | 236686 |
| chr_4 | g14389 | g14389.t1 | TTS | g14389.t1 | 236738 | 236738 |
Sequences
>g14389.t1 Gene=g14389 Length=4737
ATGAAACCTTATCGTCTTGTTCATCATCAGAGGTCAGTTTACAATGAAGATTTGTTGTTG
AATCCAAAAGAGCATCCAAATATCAAAAAGGGTGATATTGTAGAAATTTTTCATCAAGAT
GAAAATGATGAAAAAGGAAAACAACGCTGTCGTTTGCTTCTTCAAGTTTCACTGACAAAA
GACACTCAAGGAAGAAATGATTACATCAGTGTGGAATCTTCAATAGCTTTAACTTTTAAT
TTAAAGAACTATGGTGATGTTTTCATGCAAGTCGTTAATCCAGCTAGTGTTGCCTTAGAT
AGTGTAGAAATAACGTTCAAAGATCAATATATGGGACGATCAGAAATGTGGCGTTTAAAG
CAGCATCTTACAAAAACCTGTGTCTACATTAATAAAAAAATCGAATACTGTGATTCCATT
CGTTGTCAAGTTTATGAAATGTGGAGTTTTGGAGAAAGAGTATCATGTGGAGTTATTACA
GAAGATACAAAAGTTGTTTTTAGGAGTAGTACCTCCATGGTTTACCTATTTATGCAAATG
AGCTCTGAAATGTGGGACTTTGATATTCATGGAGACCTTTATTTTGAAAAGGCTGTAAAT
GGTTTTTTGACAGACCTTTTTGCAAAATGGAAAAAACAAGGCAGCAATCATGAAGTCACA
ATTGTGTTATTTTCACGAACATTTTATGCTGCAAAAAATCTGGAGGAATTTCCTGAACAT
ATGCGAGATTGTTTGCAAATGGATTATAAAGGAAGATTTTATGAGGATTTTTATCGTGTA
GCCATTCAAAATGAAAGAAACGATGATTGGAGCACAATTTTAGTGCAACTTAGACGTCTT
TTTACATCATACAAAGATATTGTATTGAAATATCATGAAAGACCAGGAGAAATAATACCA
ATGGCAATAAATTCAACCGCTGCCCAGGGCAATTTTCTTGAAGTTTTAAATATCTCATTG
AATGTCTTTGAAAAGCATTATCTTGATAGAAGTTTTGATCGAACTGGTCAATTATCAATT
GTAATAACACCAGGAGTTGGCGTATTCGAAGTAGATCGAGAGCTTACTAATATTACTAAG
CAGAGAATTATTGATAATGGCGTTGGAAGTGATTTAGTTTGTGTTGGTGAACAGCCATTA
CATGCAGTACCTCTTTTGAAATTTCACAACAAAGATCCTTCATTAAATTCAGTAGATGAT
TATTCAATGCCACACTGGGTTAATCTGAGTTTTTATTCTACCAATAAAAAAGTCGCTTAT
TCAACTTTCATACCACGTATTAAATTACCTCCACAAATTCGTAAAAATGAAGATGACAAT
GAAGTGTTGCATGTAAAAAAACTCAAAGAAGAAATTAATTTTTATACTGACACAAATACT
GAATATATTCATAATTCACTTTTTGATTATGATGCATATGATGCACAAGTTTTTGCATTT
CCCAGTCATACAACACTTATACAAAAAACTAAAAAATCAAGTATAGATGGTTTCAATTCA
TATTCAGTGTCAGTGACATCAAATAATGGCAGCAATGGATTTCAACGAGTCCGTAAGATG
TCTGATCCTGATATTTATCATGCTTGCAGTTTTTCAGAAACAAATGGTGCTTTACGTGAA
TCATTATCACAATCAACGTTGACAGCTACAAAAACAAGAAAATTGCTACCAGTACGTACT
GGACGAGCACTTATAAATCCATTTGATCCATCACATGTGACTATCAAATTGACTTCTAAT
AGAAGAAGATGGTCGCACATTTTTCCAAAAGGTCCAACAGGTTTTTTAATTCAACAACAT
CATTATCAAGCTGTTCCGTCAAAAACTGTACAAGAATCTGAAATGGTGCGCGACGATTCA
TTTAATTTTATCAATATCGATGATTATAATCACACTGCCATGTGTACACACAACAATAGT
AATGGAAATATCAATGGCAATATGGGTAGAAGACAACATAGCAGCTTATCAACAAGATCT
TTTGAAAGATTCGAAGAAGATTTCAATAGATTAAGTGCAAATAATACTCCAATAGACAGA
GGAAGTTTTCTAAATGATTCTTCATTTTTAAAAACAAGAAAAGCTTCTGCTTTGAGTTCA
CCATTTAATCAACCAGCAGCAACATCAACACCATCAAAAACACTTCTTTGGGGAGCTACA
GGTGAACAATGTTGGACACCTCAATTGACTACTGCTGGAAAAGTCATCATAGGAGTGGAC
TGGAAGTCATTGACAATTCCGGCATGTCTTCCAATCACCACCGATTATTTTCCGGATAAA
CGATCGCTCAATAATGACTACGTTTTATCAATCTACACATTACTGCCAGATGATGTTAAT
GCTGATTTTATGCGATTAAGAGCTGTTCAGAGAAAGCCATTATCGACTGAAGAAGTTTTT
AAAGAGCTTGTGTCACAAAGATTAGCTCAAGGATTTCAATTAATAATTCTTCCTGATCAT
CTTCAAACACAAAATAAACCAGCAACTGCACCTTGCTGTGGTGGAAGTATACAAATTAAA
GGGTCTGTAGTAGCACCAATAACAATTGAACAGACTAAAGAATATTTACTTTCGATTGGA
CGAATATTTCATAAAATTGCCCTAAATGATAAGGAAATAAAAATTACAAGATATCGACCA
CGCCATCCTTATCCTCCTATAAATGTTGATTATCGTTATCGTTTTCATGCACCAAATCAT
GATACTTATGAAGTCTCAGGTGTGAATTTTACAACTGAAAAACTAGAAAACTTTAATTGG
AATCATATGGATCAATATATTTGTACCAGAGGCGATTTTGATTTTATGCTACTCGAGCAA
ATGAAATATTGGCGATATAGAATGTATTTACTGCCTAAAGAAGATCTAATAATGAAAAGA
ATTATGGAGGAAAATATTGAAAATTGTGACATCTATACACAGGAAACAACTTTAGCTATG
CAAAAAATTTTAATTGAAGATTTTTTGAAATTTGTTGAAACTCATTTAAATAAGATTAAA
AGAGTGAAGAAACCAAAAGGAAGTCCGACAACTTCACACTTGACGAGAAGAAGACACAGT
ACGAGTACGAGTATTCATTCGAGACCACCGGCTGTAAATCAAAATCAGGCACTTCAAAAT
TCTCCATTCAGAGAAAGAGTTGGCAGCAATCGTTTTCCCGAAAGATTAAGACCAAGCTCA
AATGTTTCCATGAATAGATCAGGATCTAAAATTGAAACGCGATCGGCTTTAATGCAGCTG
CAGGCTTCGATGGCTGAGAAAAATTCGAAAACAGAAAATAATGATGACAATGATGATTGT
TTTGCTCCTGATCCACCGAAATTCAAATCATCATCACCCATTAATGAGATATTTGATGCC
TTTAAAAATCCAAATACTGGTGTTGGATTTCTTGAACCATTGCCGAGTATGCCTTCACTT
ACCTTTTCATCTTATGATGCTATCATATGGATTTTCAATCGAATTGAAGATGTCACTAAT
CCATTAGATATTTTGGAAACAATGAAAGAACAACGGTTAATTTGTCATGCTAGTGGAGAT
TTTAATATGCCAATTATTCCTGGTTTTTATATGTATTACATAGCACAACAAGATGTCAAT
TCATCAGATTATCGAGCACCGTTGGGAGATCGAGATGCATTTAGTTTTGAATGGATGGAA
GTAGAAATTCCAATGAAATATATTATGTCAACATTAAAACCATCAGTTGCTACTAAAACT
ACACCCAATGAAAATGATGTGCCAGCATTTTTGAAAGAGGAAATCGAGAGAAGAGATTTA
GATGGAAAATTGTACAAACATTCGCATTTAGAAGTTGATTTATCTGCAAAAAGTGACAGA
ATTGAATGGGGTCATGCAAGATATCACAAACATTTTATTCCTGGGCATGCCTTTGAAATT
GTAGTTCAATGGTTAGTTGCATCTGGACCTATTGTTTATGATCTGATTTACAACTGGAGT
AGAAAAGCAACACAATGTAGTTATCAATTGGTTCCAATTCCATGCGATCCTCTTGCGGAA
CCTTTTAGTTTATTTGAAAAATCAGATCCACTTCGAGGTCCAATTTTTGTGCCACTAAAT
ATTGATTGTCTACATCAAAATCGTTCAAGCATTTTTGAAGATTTTAAGCGTGATACATGG
GGTGAAAGGCTGCTTTTATTTCAAGAAGAAATTGCTAAACGTTTTGGTTTTATTCCTAGA
GAATGTGAAACTAAAACCGGTTCAAATGATATCACACCAGATTGGCAATACATTCATACT
AGTGGCAATATGTTTGTTGTAATAACTTCATCAACCAATAAAGTACGTAACACAATGAGA
CGTTTAGGAATGGGTAATGGTAGTCATTCTAGTCCAACTGATAGCTTAAAATCCCAGAAG
CGTCTTAAAAATTATTATCATGTTCAACAACAACAACAACAAACTTCAGTACCACAAAAA
GAAAATTATATTAATAGACATGTACAAAATAATTCTAATGGCAGTCGAGATTCAGATGAA
GAAGGCTTTAGTAGCATGCAAAGGAAACATCCAATTGGATTTCTTTGGCAATGGAATCAT
ATGATTGCTAATAAAAAATGGAAATCATTGTCTATCAATGGAACAGATGAAATATTTCAA
CTACGGATGCTACGTGACTTTAAAAATTTTTGCTCTAATGCTGATGGAAGGCTTATGAAA
TTTTGGGATCAAGCTTGGGAAAAGAAAGAAAAATTGTCACTATTAGGTGCAAATTAA
>g14389.t1 Gene=g14389 Length=1578
MKPYRLVHHQRSVYNEDLLLNPKEHPNIKKGDIVEIFHQDENDEKGKQRCRLLLQVSLTK
DTQGRNDYISVESSIALTFNLKNYGDVFMQVVNPASVALDSVEITFKDQYMGRSEMWRLK
QHLTKTCVYINKKIEYCDSIRCQVYEMWSFGERVSCGVITEDTKVVFRSSTSMVYLFMQM
SSEMWDFDIHGDLYFEKAVNGFLTDLFAKWKKQGSNHEVTIVLFSRTFYAAKNLEEFPEH
MRDCLQMDYKGRFYEDFYRVAIQNERNDDWSTILVQLRRLFTSYKDIVLKYHERPGEIIP
MAINSTAAQGNFLEVLNISLNVFEKHYLDRSFDRTGQLSIVITPGVGVFEVDRELTNITK
QRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDPSLNSVDDYSMPHWVNLSFYSTNKKVAY
STFIPRIKLPPQIRKNEDDNEVLHVKKLKEEINFYTDTNTEYIHNSLFDYDAYDAQVFAF
PSHTTLIQKTKKSSIDGFNSYSVSVTSNNGSNGFQRVRKMSDPDIYHACSFSETNGALRE
SLSQSTLTATKTRKLLPVRTGRALINPFDPSHVTIKLTSNRRRWSHIFPKGPTGFLIQQH
HYQAVPSKTVQESEMVRDDSFNFINIDDYNHTAMCTHNNSNGNINGNMGRRQHSSLSTRS
FERFEEDFNRLSANNTPIDRGSFLNDSSFLKTRKASALSSPFNQPAATSTPSKTLLWGAT
GEQCWTPQLTTAGKVIIGVDWKSLTIPACLPITTDYFPDKRSLNNDYVLSIYTLLPDDVN
ADFMRLRAVQRKPLSTEEVFKELVSQRLAQGFQLIILPDHLQTQNKPATAPCCGGSIQIK
GSVVAPITIEQTKEYLLSIGRIFHKIALNDKEIKITRYRPRHPYPPINVDYRYRFHAPNH
DTYEVSGVNFTTEKLENFNWNHMDQYICTRGDFDFMLLEQMKYWRYRMYLLPKEDLIMKR
IMEENIENCDIYTQETTLAMQKILIEDFLKFVETHLNKIKRVKKPKGSPTTSHLTRRRHS
TSTSIHSRPPAVNQNQALQNSPFRERVGSNRFPERLRPSSNVSMNRSGSKIETRSALMQL
QASMAEKNSKTENNDDNDDCFAPDPPKFKSSSPINEIFDAFKNPNTGVGFLEPLPSMPSL
TFSSYDAIIWIFNRIEDVTNPLDILETMKEQRLICHASGDFNMPIIPGFYMYYIAQQDVN
SSDYRAPLGDRDAFSFEWMEVEIPMKYIMSTLKPSVATKTTPNENDVPAFLKEEIERRDL
DGKLYKHSHLEVDLSAKSDRIEWGHARYHKHFIPGHAFEIVVQWLVASGPIVYDLIYNWS
RKATQCSYQLVPIPCDPLAEPFSLFEKSDPLRGPIFVPLNIDCLHQNRSSIFEDFKRDTW
GERLLLFQEEIAKRFGFIPRECETKTGSNDITPDWQYIHTSGNMFVVITSSTNKVRNTMR
RLGMGNGSHSSPTDSLKSQKRLKNYYHVQQQQQQTSVPQKENYINRHVQNNSNGSRDSDE
EGFSSMQRKHPIGFLWQWNHMIANKKWKSLSINGTDEIFQLRMLRDFKNFCSNADGRLMK
FWDQAWEKKEKLSLLGAN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g14389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1002 | 1023 | - |
| 8 | g14389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1002 | 1048 | - |
| 7 | g14389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1024 | 1046 | - |
| 4 | g14389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1470 | 1504 | - |
| 6 | g14389.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1470 | 1500 | - |
| 2 | g14389.t1 | PANTHER | PTHR13179 | DEP DOMAIN CONTAINING PROTEIN 5 | 4 | 524 | 0.0 |
| 3 | g14389.t1 | PANTHER | PTHR13179 | DEP DOMAIN CONTAINING PROTEIN 5 | 528 | 1571 | 0.0 |
| 1 | g14389.t1 | Pfam | PF12257 | Vacuolar membrane-associated protein Iml1 | 102 | 388 | 1.7E-111 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005096 | GTPase activator activity | MF |
KEGG
Orthology
- This transcript has been annotated with K20404 (SEA/GATOR complex protein SEA1/DEPDC5)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
