General information for g14389.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as GATOR complex protein Iml1.
Parent gene
- The parent gene g14389 is located on chr_4: 235182..236686 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14389 | g14389.t2 | TSS | g14389.t2 | 228204 | 228204 |
| chr_4 | g14389 | g14389.t2 | isoform | g14389.t2 | 228456 | 236686 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon1 | 228456 | 228546 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS1 | 228456 | 228546 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon2 | 228838 | 229057 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS2 | 228838 | 229057 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon3 | 229478 | 229596 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS3 | 229478 | 229596 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon4 | 230085 | 230740 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS4 | 230085 | 230740 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon5 | 230799 | 230846 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS5 | 230799 | 230846 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon6 | 230909 | 231839 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS6 | 230909 | 231839 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon7 | 231894 | 232022 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS7 | 231894 | 232022 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon8 | 233160 | 233377 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS8 | 233160 | 233377 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon9 | 233434 | 233820 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS9 | 233434 | 233820 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon10 | 233886 | 234086 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS10 | 233886 | 234086 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon11 | 234336 | 234425 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS11 | 234336 | 234425 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon12 | 234716 | 234783 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS12 | 234716 | 234783 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon13 | 235006 | 235128 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS13 | 235006 | 235128 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon14 | 235182 | 235827 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS14 | 235182 | 235827 |
| chr_4 | g14389 | g14389.t2 | exon | g14389.t2.exon15 | 235895 | 236686 |
| chr_4 | g14389 | g14389.t2 | cds | g14389.t2.CDS15 | 235895 | 236686 |
| chr_4 | g14389 | g14389.t2 | TTS | g14389.t2 | 236738 | 236738 |
Sequences
>g14389.t2 Gene=g14389 Length=4719
ATGAAACCTTATCGTCTTGTTCATCATCAGAGGTCAGTTTACAATGAAGATTTGTTGTTG
AATCCAAAAGAGCATCCAAATATCAAAAAGGGTGATATTGTAGAAATTTTTCATCAAGAT
GAAAATGATGAAAAAGGAAAACAACGCTGTCGTTTGCTTCTTCAAGTTTCACTGACAAAA
GACACTCAAGGAAGAAATGATTACATCAGTGTGGAATCTTCAATAGCTTTAACTTTTAAT
TTAAAGAACTATGGTGATGTTTTCATGCAAGTCGTTAATCCAGCTAGTGTTGCCTTAGAT
AGTGTAGAAATAACGTTCAAAGATCAATATATGGGACGATCAGAAATGTGGCGTTTAAAG
CAGCATCTTACAAAAACCTGTGTCTACATTAATAAAAAAATCGAATACTGTGATTCCATT
CGTTGTCAAGTTTATGAAATGTGGAGTTTTGGAGAAAGAGTATCATGTGGAGTTATTACA
GAAGATACAAAAGTTGTTTTTAGGAGTAGTACCTCCATGGTTTACCTATTTATGCAAATG
AGCTCTGAAATGTGGGACTTTGATATTCATGGAGACCTTTATTTTGAAAAGGCTGTAAAT
GGTTTTTTGACAGACCTTTTTGCAAAATGGAAAAAACAAGGCAGCAATCATGAAGTCACA
ATTGTGTTATTTTCACGAACATTTTATGCTGCAAAAAATCTGGAGGAATTTCCTGAACAT
ATGCGAGATTGTTTGCAAATGGATTATAAAGGAAGATTTTATGAGGATTTTTATCGTGTA
GCCATTCAAAATGAAAGAAACGATGATTGGAGCACAATTTTAGTGCAACTTAGACGTCTT
TTTACATCATACAAAGATATTGTATTGAAATATCATGAAAGACCAGGAGAAATAATACCA
ATGGCAATAAATTCAACCGCTGCCCAGGGCAATTTTCTTGAAGTTTTAAATATCTCATTG
AATGTCTTTGAAAAGCATTATCTTGATAGAAGTTTTGATCGAACTGGTCAATTATCAATT
GTAATAACACCAGGAGTTGGCGTATTCGAAGTAGATCGAGAGCTTACTAATATTACTAAG
CAGAGAATTATTGATAATGGCGTTGGAAGTGATTTAGTTTGTGTTGGTGAACAGCCATTA
CATGCAGTACCTCTTTTGAAATTTCACAACAAAGATCCTTCATTAAATTCAGTAGATGAT
TATTCAATGCCACACTGGGTTAATCTGAGTTTTTATTCTACCAATAAAAAAGTCGCTTAT
TCAACTTTCATACCACGTATTAAATTACCTCCACAAATTCGTAAAAATGAAGATGACAAT
GAAGTGTTGCATGTAAAAAAACTCAAAGAAGAAATTAATTTTTATACTGACACAAATACT
GAATATATTCATAATTCACTTTTTGATTATGATGCATATGATGCACAAGTTTTTGCATTT
CCCAGTCATACAACACTTATACAAAAAACTAAAAAATCAAGTATAGATGGTTTCAATTCA
TATTCAGTGTCAGTGACATCAAATAATGGCAGCAATGGATTTCAACGAGTCCGTAAGATG
TCTGATCCTGATATTTATCATGCTTGCAGTTTTTCAGAAACAAATGGTGCTTTACGTGAA
TCATTATCACAATCAACGTTGACAGCTACAAAAACAAGAAAATTGCTACCAGTACGTACT
GGACGAGCACTTATAAATCCATTTGATCCATCACATGTGACTATCAAATTGACTTCTAAT
AGAAGAAGATGGTCGCACATTTTTCCAAAAGGTCCAACAGGTTTTTTAATTCAACAACAT
CATTATCAAGCTGTTCCGTCAAAAACTGTACAAGAATCTGAAATGGTGCGCGACGATTCA
TTTAATTTTATCAATATCGATGATTATAATCACACTGCCATGTGTACACACAACAATAGT
AATGGAAATATCAATGGCAATATGGGTAGAAGACAACATAGCAGCTTATCAACAAGATCT
TTTGAAAGATTCGAAGAAGATTTCAATAGATTAAGTGCAAATAATACTCCAATAGACAGA
GGAAGTTTTCTAAATGATTCTTCATTTTTAAAAACAAGAAAAGCTTCTGCTTTGAGTTCA
CCATTTAATCAACCAGCAGCAACATCAACACCATCAAAAACACTTCTTTGGGGAGCTACA
GGTGAACAATGTTGGACACCTCAATTGACTACTGGAGTGGACTGGAAGTCATTGACAATT
CCGGCATGTCTTCCAATCACCACCGATTATTTTCCGGATAAACGATCGCTCAATAATGAC
TACGTTTTATCAATCTACACATTACTGCCAGATGATGTTAATGCTGATTTTATGCGATTA
AGAGCTGTTCAGAGAAAGCCATTATCGACTGAAGAAGTTTTTAAAGAGCTTGTGTCACAA
AGATTAGCTCAAGGATTTCAATTAATAATTCTTCCTGATCATCTTCAAACACAAAATAAA
CCAGCAACTGCACCTTGCTGTGGTGGAAGTATACAAATTAAAGGGTCTGTAGTAGCACCA
ATAACAATTGAACAGACTAAAGAATATTTACTTTCGATTGGACGAATATTTCATAAAATT
GCCCTAAATGATAAGGAAATAAAAATTACAAGATATCGACCACGCCATCCTTATCCTCCT
ATAAATGTTGATTATCGTTATCGTTTTCATGCACCAAATCATGATACTTATGAAGTCTCA
GGTGTGAATTTTACAACTGAAAAACTAGAAAACTTTAATTGGAATCATATGGATCAATAT
ATTTGTACCAGAGGCGATTTTGATTTTATGCTACTCGAGCAAATGAAATATTGGCGATAT
AGAATGTATTTACTGCCTAAAGAAGATCTAATAATGAAAAGAATTATGGAGGAAAATATT
GAAAATTGTGACATCTATACACAGGAAACAACTTTAGCTATGCAAAAAATTTTAATTGAA
GATTTTTTGAAATTTGTTGAAACTCATTTAAATAAGATTAAAAGAGTGAAGAAACCAAAA
GGAAGTCCGACAACTTCACACTTGACGAGAAGAAGACACAGTACGAGTACGAGTATTCAT
TCGAGACCACCGGCTGTAAATCAAAATCAGGCACTTCAAAATTCTCCATTCAGAGAAAGA
GTTGGCAGCAATCGTTTTCCCGAAAGATTAAGACCAAGCTCAAATGTTTCCATGAATAGA
TCAGGATCTAAAATTGAAACGCGATCGGCTTTAATGCAGCTGCAGGCTTCGATGGCTGAG
AAAAATTCGAAAACAGAAAATAATGATGACAATGATGATTGTTTTGCTCCTGATCCACCG
AAATTCAAATCATCATCACCCATTAATGAGATATTTGATGCCTTTAAAAATCCAAATACT
GGTGTTGGATTTCTTGAACCATTGCCGAGTATGCCTTCACTTACCTTTTCATCTTATGAT
GCTATCATATGGATTTTCAATCGAATTGAAGATGTCACTAATCCATTAGATATTTTGGAA
ACAATGAAAGAACAACGGTTAATTTGTCATGCTAGTGGAGATTTTAATATGCCAATTATT
CCTGGTTTTTATATGTATTACATAGCACAACAAGATGTCAATTCATCAGATTATCGAGCA
CCGTTGGGAGATCGAGATGCATTTAGTTTTGAATGGATGGAAGTAGAAATTCCAATGAAA
TATATTATGTCAACATTAAAACCATCAGTTGCTACTAAAACTACACCCAATGAAAATGAT
GTGCCAGCATTTTTGAAAGAGGAAATCGAGAGAAGAGATTTAGATGGAAAATTGTACAAA
CATTCGCATTTAGAAGTTGATTTATCTGCAAAAAGTGACAGAATTGAATGGGGTCATGCA
AGATATCACAAACATTTTATTCCTGGGCATGCCTTTGAAATTGTAGTTCAATGGTTAGTT
GCATCTGGACCTATTGTTTATGATCTGATTTACAACTGGAGTAGAAAAGCAACACAATGT
AGTTATCAATTGGTTCCAATTCCATGCGATCCTCTTGCGGAACCTTTTAGTTTATTTGAA
AAATCAGATCCACTTCGAGGTCCAATTTTTGTGCCACTAAATATTGATTGTCTACATCAA
AATCGTTCAAGCATTTTTGAAGATTTTAAGCGTGATACATGGGGTGAAAGGCTGCTTTTA
TTTCAAGAAGAAATTGCTAAACGTTTTGGTTTTATTCCTAGAGAATGTGAAACTAAAACC
GGTTCAAATGATATCACACCAGATTGGCAATACATTCATACTAGTGGCAATATGTTTGTT
GTAATAACTTCATCAACCAATAAAGTACGTAACACAATGAGACGTTTAGGAATGGGTAAT
GGTAGTCATTCTAGTCCAACTGATAGCTTAAAATCCCAGAAGCGTCTTAAAAATTATTAT
CATGTTCAACAACAACAACAACAAACTTCAGTACCACAAAAAGAAAATTATATTAATAGA
CATGTACAAAATAATTCTAATGGCAGTCGAGATTCAGATGAAGAAGGCTTTAGTAGCATG
CAAAGGAAACATCCAATTGGATTTCTTTGGCAATGGAATCATATGATTGCTAATAAAAAA
TGGAAATCATTGTCTATCAATGGAACAGATGAAATATTTCAACTACGGATGCTACGTGAC
TTTAAAAATTTTTGCTCTAATGCTGATGGAAGGCTTATGAAATTTTGGGATCAAGCTTGG
GAAAAGAAAGAAAAATTGTCACTATTAGGTGCAAATTAA
>g14389.t2 Gene=g14389 Length=1572
MKPYRLVHHQRSVYNEDLLLNPKEHPNIKKGDIVEIFHQDENDEKGKQRCRLLLQVSLTK
DTQGRNDYISVESSIALTFNLKNYGDVFMQVVNPASVALDSVEITFKDQYMGRSEMWRLK
QHLTKTCVYINKKIEYCDSIRCQVYEMWSFGERVSCGVITEDTKVVFRSSTSMVYLFMQM
SSEMWDFDIHGDLYFEKAVNGFLTDLFAKWKKQGSNHEVTIVLFSRTFYAAKNLEEFPEH
MRDCLQMDYKGRFYEDFYRVAIQNERNDDWSTILVQLRRLFTSYKDIVLKYHERPGEIIP
MAINSTAAQGNFLEVLNISLNVFEKHYLDRSFDRTGQLSIVITPGVGVFEVDRELTNITK
QRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDPSLNSVDDYSMPHWVNLSFYSTNKKVAY
STFIPRIKLPPQIRKNEDDNEVLHVKKLKEEINFYTDTNTEYIHNSLFDYDAYDAQVFAF
PSHTTLIQKTKKSSIDGFNSYSVSVTSNNGSNGFQRVRKMSDPDIYHACSFSETNGALRE
SLSQSTLTATKTRKLLPVRTGRALINPFDPSHVTIKLTSNRRRWSHIFPKGPTGFLIQQH
HYQAVPSKTVQESEMVRDDSFNFINIDDYNHTAMCTHNNSNGNINGNMGRRQHSSLSTRS
FERFEEDFNRLSANNTPIDRGSFLNDSSFLKTRKASALSSPFNQPAATSTPSKTLLWGAT
GEQCWTPQLTTGVDWKSLTIPACLPITTDYFPDKRSLNNDYVLSIYTLLPDDVNADFMRL
RAVQRKPLSTEEVFKELVSQRLAQGFQLIILPDHLQTQNKPATAPCCGGSIQIKGSVVAP
ITIEQTKEYLLSIGRIFHKIALNDKEIKITRYRPRHPYPPINVDYRYRFHAPNHDTYEVS
GVNFTTEKLENFNWNHMDQYICTRGDFDFMLLEQMKYWRYRMYLLPKEDLIMKRIMEENI
ENCDIYTQETTLAMQKILIEDFLKFVETHLNKIKRVKKPKGSPTTSHLTRRRHSTSTSIH
SRPPAVNQNQALQNSPFRERVGSNRFPERLRPSSNVSMNRSGSKIETRSALMQLQASMAE
KNSKTENNDDNDDCFAPDPPKFKSSSPINEIFDAFKNPNTGVGFLEPLPSMPSLTFSSYD
AIIWIFNRIEDVTNPLDILETMKEQRLICHASGDFNMPIIPGFYMYYIAQQDVNSSDYRA
PLGDRDAFSFEWMEVEIPMKYIMSTLKPSVATKTTPNENDVPAFLKEEIERRDLDGKLYK
HSHLEVDLSAKSDRIEWGHARYHKHFIPGHAFEIVVQWLVASGPIVYDLIYNWSRKATQC
SYQLVPIPCDPLAEPFSLFEKSDPLRGPIFVPLNIDCLHQNRSSIFEDFKRDTWGERLLL
FQEEIAKRFGFIPRECETKTGSNDITPDWQYIHTSGNMFVVITSSTNKVRNTMRRLGMGN
GSHSSPTDSLKSQKRLKNYYHVQQQQQQTSVPQKENYINRHVQNNSNGSRDSDEEGFSSM
QRKHPIGFLWQWNHMIANKKWKSLSINGTDEIFQLRMLRDFKNFCSNADGRLMKFWDQAW
EKKEKLSLLGAN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g14389.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 996 | 1042 | - |
| 7 | g14389.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 996 | 1017 | - |
| 4 | g14389.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1018 | 1040 | - |
| 6 | g14389.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1464 | 1494 | - |
| 8 | g14389.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1464 | 1498 | - |
| 3 | g14389.t2 | PANTHER | PTHR13179 | DEP DOMAIN CONTAINING PROTEIN 5 | 4 | 524 | 0.0 |
| 2 | g14389.t2 | PANTHER | PTHR13179 | DEP DOMAIN CONTAINING PROTEIN 5 | 533 | 1565 | 0.0 |
| 1 | g14389.t2 | Pfam | PF12257 | Vacuolar membrane-associated protein Iml1 | 102 | 388 | 1.7E-111 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005096 | GTPase activator activity | MF |
KEGG
Orthology
- This transcript has been annotated with K20404 (SEA/GATOR complex protein SEA1/DEPDC5)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
