General information for g14389.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as GATOR complex protein Iml1.
Parent gene
- The parent gene g14389 is located on chr_4: 235182..236686 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14389 | g14389.t4 | TSS | g14389.t4 | 228204 | 228204 |
| chr_4 | g14389 | g14389.t4 | isoform | g14389.t4 | 228456 | 236686 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon1 | 228456 | 228546 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS1 | 228456 | 228546 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon2 | 228838 | 229057 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS2 | 228838 | 229057 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon3 | 229478 | 229596 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS3 | 229478 | 229596 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon4 | 230085 | 230740 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS4 | 230085 | 230740 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon5 | 230799 | 230846 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS5 | 230799 | 230846 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon6 | 230909 | 231839 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS6 | 230909 | 231839 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon7 | 231894 | 232022 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS7 | 231894 | 232022 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon8 | 233160 | 233377 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS8 | 233160 | 233377 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon9 | 233434 | 233820 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS9 | 233434 | 233820 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon10 | 233886 | 234086 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS10 | 233886 | 234086 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon11 | 234716 | 234783 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS11 | 234716 | 234783 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon12 | 235006 | 235128 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS12 | 235006 | 235128 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon13 | 235182 | 235827 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS13 | 235182 | 235827 |
| chr_4 | g14389 | g14389.t4 | exon | g14389.t4.exon14 | 235895 | 236686 |
| chr_4 | g14389 | g14389.t4 | cds | g14389.t4.CDS14 | 235895 | 236686 |
| chr_4 | g14389 | g14389.t4 | TTS | g14389.t4 | 236738 | 236738 |
Sequences
>g14389.t4 Gene=g14389 Length=4629
ATGAAACCTTATCGTCTTGTTCATCATCAGAGGTCAGTTTACAATGAAGATTTGTTGTTG
AATCCAAAAGAGCATCCAAATATCAAAAAGGGTGATATTGTAGAAATTTTTCATCAAGAT
GAAAATGATGAAAAAGGAAAACAACGCTGTCGTTTGCTTCTTCAAGTTTCACTGACAAAA
GACACTCAAGGAAGAAATGATTACATCAGTGTGGAATCTTCAATAGCTTTAACTTTTAAT
TTAAAGAACTATGGTGATGTTTTCATGCAAGTCGTTAATCCAGCTAGTGTTGCCTTAGAT
AGTGTAGAAATAACGTTCAAAGATCAATATATGGGACGATCAGAAATGTGGCGTTTAAAG
CAGCATCTTACAAAAACCTGTGTCTACATTAATAAAAAAATCGAATACTGTGATTCCATT
CGTTGTCAAGTTTATGAAATGTGGAGTTTTGGAGAAAGAGTATCATGTGGAGTTATTACA
GAAGATACAAAAGTTGTTTTTAGGAGTAGTACCTCCATGGTTTACCTATTTATGCAAATG
AGCTCTGAAATGTGGGACTTTGATATTCATGGAGACCTTTATTTTGAAAAGGCTGTAAAT
GGTTTTTTGACAGACCTTTTTGCAAAATGGAAAAAACAAGGCAGCAATCATGAAGTCACA
ATTGTGTTATTTTCACGAACATTTTATGCTGCAAAAAATCTGGAGGAATTTCCTGAACAT
ATGCGAGATTGTTTGCAAATGGATTATAAAGGAAGATTTTATGAGGATTTTTATCGTGTA
GCCATTCAAAATGAAAGAAACGATGATTGGAGCACAATTTTAGTGCAACTTAGACGTCTT
TTTACATCATACAAAGATATTGTATTGAAATATCATGAAAGACCAGGAGAAATAATACCA
ATGGCAATAAATTCAACCGCTGCCCAGGGCAATTTTCTTGAAGTTTTAAATATCTCATTG
AATGTCTTTGAAAAGCATTATCTTGATAGAAGTTTTGATCGAACTGGTCAATTATCAATT
GTAATAACACCAGGAGTTGGCGTATTCGAAGTAGATCGAGAGCTTACTAATATTACTAAG
CAGAGAATTATTGATAATGGCGTTGGAAGTGATTTAGTTTGTGTTGGTGAACAGCCATTA
CATGCAGTACCTCTTTTGAAATTTCACAACAAAGATCCTTCATTAAATTCAGTAGATGAT
TATTCAATGCCACACTGGGTTAATCTGAGTTTTTATTCTACCAATAAAAAAGTCGCTTAT
TCAACTTTCATACCACGTATTAAATTACCTCCACAAATTCGTAAAAATGAAGATGACAAT
GAAGTGTTGCATGTAAAAAAACTCAAAGAAGAAATTAATTTTTATACTGACACAAATACT
GAATATATTCATAATTCACTTTTTGATTATGATGCATATGATGCACAAGTTTTTGCATTT
CCCAGTCATACAACACTTATACAAAAAACTAAAAAATCAAGTATAGATGGTTTCAATTCA
TATTCAGTGTCAGTGACATCAAATAATGGCAGCAATGGATTTCAACGAGTCCGTAAGATG
TCTGATCCTGATATTTATCATGCTTGCAGTTTTTCAGAAACAAATGGTGCTTTACGTGAA
TCATTATCACAATCAACGTTGACAGCTACAAAAACAAGAAAATTGCTACCAGTACGTACT
GGACGAGCACTTATAAATCCATTTGATCCATCACATGTGACTATCAAATTGACTTCTAAT
AGAAGAAGATGGTCGCACATTTTTCCAAAAGGTCCAACAGGTTTTTTAATTCAACAACAT
CATTATCAAGCTGTTCCGTCAAAAACTGTACAAGAATCTGAAATGGTGCGCGACGATTCA
TTTAATTTTATCAATATCGATGATTATAATCACACTGCCATGTGTACACACAACAATAGT
AATGGAAATATCAATGGCAATATGGGTAGAAGACAACATAGCAGCTTATCAACAAGATCT
TTTGAAAGATTCGAAGAAGATTTCAATAGATTAAGTGCAAATAATACTCCAATAGACAGA
GGAAGTTTTCTAAATGATTCTTCATTTTTAAAAACAAGAAAAGCTTCTGCTTTGAGTTCA
CCATTTAATCAACCAGCAGCAACATCAACACCATCAAAAACACTTCTTTGGGGAGCTACA
GGTGAACAATGTTGGACACCTCAATTGACTACTGGAGTGGACTGGAAGTCATTGACAATT
CCGGCATGTCTTCCAATCACCACCGATTATTTTCCGGATAAACGATCGCTCAATAATGAC
TACGTTTTATCAATCTACACATTACTGCCAGATGATGTTAATGCTGATTTTATGCGATTA
AGAGCTGTTCAGAGAAAGCCATTATCGACTGAAGAAGTTTTTAAAGAGCTTGTGTCACAA
AGATTAGCTCAAGGATTTCAATTAATAATTCTTCCTGATCATCTTCAAACACAAAATAAA
CCAGCAACTGCACCTTGCTGTGGTGGAAGTATACAAATTAAAGGGTCTGTAGTAGCACCA
ATAACAATTGAACAGACTAAAGAATATTTACTTTCGATTGGACGAATATTTCATAAAATT
GCCCTAAATGATAAGGAAATAAAAATTACAAGATATCGACCACGCCATCCTTATCCTCCT
ATAAATGTTGATTATCGTTATCGTTTTCATGCACCAAATCATGATACTTATGAAGTCTCA
GGTGTGAATTTTACAACTGAAAAACTAGAAAACTTTAATTGGAATCATATGGATCAATAT
ATTTGTACCAGAGGCGATTTTGATTTTATGCTACTCGAGCAAATGAAATATTGGCGATAT
AGAATGTATTTACTGCCTAAAGAAGATCTAATAATGAAAAGAATTATGGAGGAAAATATT
GAAAATTGTGACATCTATACACAGGAAACAACTTTAGCTATGCAAAAAATTTTAATTGAA
GATTTTTTGAAATTTGTTGAAACTCATTTAAATAAGATTAAAAGAGTGAAGAAACCAAAA
GCACTTCAAAATTCTCCATTCAGAGAAAGAGTTGGCAGCAATCGTTTTCCCGAAAGATTA
AGACCAAGCTCAAATGTTTCCATGAATAGATCAGGATCTAAAATTGAAACGCGATCGGCT
TTAATGCAGCTGCAGGCTTCGATGGCTGAGAAAAATTCGAAAACAGAAAATAATGATGAC
AATGATGATTGTTTTGCTCCTGATCCACCGAAATTCAAATCATCATCACCCATTAATGAG
ATATTTGATGCCTTTAAAAATCCAAATACTGGTGTTGGATTTCTTGAACCATTGCCGAGT
ATGCCTTCACTTACCTTTTCATCTTATGATGCTATCATATGGATTTTCAATCGAATTGAA
GATGTCACTAATCCATTAGATATTTTGGAAACAATGAAAGAACAACGGTTAATTTGTCAT
GCTAGTGGAGATTTTAATATGCCAATTATTCCTGGTTTTTATATGTATTACATAGCACAA
CAAGATGTCAATTCATCAGATTATCGAGCACCGTTGGGAGATCGAGATGCATTTAGTTTT
GAATGGATGGAAGTAGAAATTCCAATGAAATATATTATGTCAACATTAAAACCATCAGTT
GCTACTAAAACTACACCCAATGAAAATGATGTGCCAGCATTTTTGAAAGAGGAAATCGAG
AGAAGAGATTTAGATGGAAAATTGTACAAACATTCGCATTTAGAAGTTGATTTATCTGCA
AAAAGTGACAGAATTGAATGGGGTCATGCAAGATATCACAAACATTTTATTCCTGGGCAT
GCCTTTGAAATTGTAGTTCAATGGTTAGTTGCATCTGGACCTATTGTTTATGATCTGATT
TACAACTGGAGTAGAAAAGCAACACAATGTAGTTATCAATTGGTTCCAATTCCATGCGAT
CCTCTTGCGGAACCTTTTAGTTTATTTGAAAAATCAGATCCACTTCGAGGTCCAATTTTT
GTGCCACTAAATATTGATTGTCTACATCAAAATCGTTCAAGCATTTTTGAAGATTTTAAG
CGTGATACATGGGGTGAAAGGCTGCTTTTATTTCAAGAAGAAATTGCTAAACGTTTTGGT
TTTATTCCTAGAGAATGTGAAACTAAAACCGGTTCAAATGATATCACACCAGATTGGCAA
TACATTCATACTAGTGGCAATATGTTTGTTGTAATAACTTCATCAACCAATAAAGTACGT
AACACAATGAGACGTTTAGGAATGGGTAATGGTAGTCATTCTAGTCCAACTGATAGCTTA
AAATCCCAGAAGCGTCTTAAAAATTATTATCATGTTCAACAACAACAACAACAAACTTCA
GTACCACAAAAAGAAAATTATATTAATAGACATGTACAAAATAATTCTAATGGCAGTCGA
GATTCAGATGAAGAAGGCTTTAGTAGCATGCAAAGGAAACATCCAATTGGATTTCTTTGG
CAATGGAATCATATGATTGCTAATAAAAAATGGAAATCATTGTCTATCAATGGAACAGAT
GAAATATTTCAACTACGGATGCTACGTGACTTTAAAAATTTTTGCTCTAATGCTGATGGA
AGGCTTATGAAATTTTGGGATCAAGCTTGGGAAAAGAAAGAAAAATTGTCACTATTAGGT
GCAAATTAA
>g14389.t4 Gene=g14389 Length=1542
MKPYRLVHHQRSVYNEDLLLNPKEHPNIKKGDIVEIFHQDENDEKGKQRCRLLLQVSLTK
DTQGRNDYISVESSIALTFNLKNYGDVFMQVVNPASVALDSVEITFKDQYMGRSEMWRLK
QHLTKTCVYINKKIEYCDSIRCQVYEMWSFGERVSCGVITEDTKVVFRSSTSMVYLFMQM
SSEMWDFDIHGDLYFEKAVNGFLTDLFAKWKKQGSNHEVTIVLFSRTFYAAKNLEEFPEH
MRDCLQMDYKGRFYEDFYRVAIQNERNDDWSTILVQLRRLFTSYKDIVLKYHERPGEIIP
MAINSTAAQGNFLEVLNISLNVFEKHYLDRSFDRTGQLSIVITPGVGVFEVDRELTNITK
QRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDPSLNSVDDYSMPHWVNLSFYSTNKKVAY
STFIPRIKLPPQIRKNEDDNEVLHVKKLKEEINFYTDTNTEYIHNSLFDYDAYDAQVFAF
PSHTTLIQKTKKSSIDGFNSYSVSVTSNNGSNGFQRVRKMSDPDIYHACSFSETNGALRE
SLSQSTLTATKTRKLLPVRTGRALINPFDPSHVTIKLTSNRRRWSHIFPKGPTGFLIQQH
HYQAVPSKTVQESEMVRDDSFNFINIDDYNHTAMCTHNNSNGNINGNMGRRQHSSLSTRS
FERFEEDFNRLSANNTPIDRGSFLNDSSFLKTRKASALSSPFNQPAATSTPSKTLLWGAT
GEQCWTPQLTTGVDWKSLTIPACLPITTDYFPDKRSLNNDYVLSIYTLLPDDVNADFMRL
RAVQRKPLSTEEVFKELVSQRLAQGFQLIILPDHLQTQNKPATAPCCGGSIQIKGSVVAP
ITIEQTKEYLLSIGRIFHKIALNDKEIKITRYRPRHPYPPINVDYRYRFHAPNHDTYEVS
GVNFTTEKLENFNWNHMDQYICTRGDFDFMLLEQMKYWRYRMYLLPKEDLIMKRIMEENI
ENCDIYTQETTLAMQKILIEDFLKFVETHLNKIKRVKKPKALQNSPFRERVGSNRFPERL
RPSSNVSMNRSGSKIETRSALMQLQASMAEKNSKTENNDDNDDCFAPDPPKFKSSSPINE
IFDAFKNPNTGVGFLEPLPSMPSLTFSSYDAIIWIFNRIEDVTNPLDILETMKEQRLICH
ASGDFNMPIIPGFYMYYIAQQDVNSSDYRAPLGDRDAFSFEWMEVEIPMKYIMSTLKPSV
ATKTTPNENDVPAFLKEEIERRDLDGKLYKHSHLEVDLSAKSDRIEWGHARYHKHFIPGH
AFEIVVQWLVASGPIVYDLIYNWSRKATQCSYQLVPIPCDPLAEPFSLFEKSDPLRGPIF
VPLNIDCLHQNRSSIFEDFKRDTWGERLLLFQEEIAKRFGFIPRECETKTGSNDITPDWQ
YIHTSGNMFVVITSSTNKVRNTMRRLGMGNGSHSSPTDSLKSQKRLKNYYHVQQQQQQTS
VPQKENYINRHVQNNSNGSRDSDEEGFSSMQRKHPIGFLWQWNHMIANKKWKSLSINGTD
EIFQLRMLRDFKNFCSNADGRLMKFWDQAWEKKEKLSLLGAN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 4 | g14389.t4 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1434 | 1468 | - |
| 5 | g14389.t4 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1434 | 1464 | - |
| 3 | g14389.t4 | PANTHER | PTHR13179 | DEP DOMAIN CONTAINING PROTEIN 5 | 4 | 524 | 0.0 |
| 2 | g14389.t4 | PANTHER | PTHR13179 | DEP DOMAIN CONTAINING PROTEIN 5 | 521 | 1535 | 0.0 |
| 1 | g14389.t4 | Pfam | PF12257 | Vacuolar membrane-associated protein Iml1 | 102 | 388 | 1.6E-111 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005096 | GTPase activator activity | MF |
KEGG
Orthology
- This transcript has been annotated with K20404 (SEA/GATOR complex protein SEA1/DEPDC5)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
