General information for g14389.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as GATOR complex protein Iml1.
Parent gene
- The parent gene g14389 is located on chr_4: 235182..236686 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14389 | g14389.t5 | TSS | g14389.t5 | 228204 | 228204 |
| chr_4 | g14389 | g14389.t5 | isoform | g14389.t5 | 228456 | 231427 |
| chr_4 | g14389 | g14389.t5 | exon | g14389.t5.exon1 | 228456 | 228546 |
| chr_4 | g14389 | g14389.t5 | cds | g14389.t5.CDS1 | 228456 | 228546 |
| chr_4 | g14389 | g14389.t5 | exon | g14389.t5.exon2 | 228838 | 229057 |
| chr_4 | g14389 | g14389.t5 | cds | g14389.t5.CDS2 | 228838 | 229057 |
| chr_4 | g14389 | g14389.t5 | exon | g14389.t5.exon3 | 229478 | 229596 |
| chr_4 | g14389 | g14389.t5 | cds | g14389.t5.CDS3 | 229478 | 229596 |
| chr_4 | g14389 | g14389.t5 | exon | g14389.t5.exon4 | 230085 | 230740 |
| chr_4 | g14389 | g14389.t5 | cds | g14389.t5.CDS4 | 230085 | 230740 |
| chr_4 | g14389 | g14389.t5 | exon | g14389.t5.exon5 | 230799 | 230846 |
| chr_4 | g14389 | g14389.t5 | cds | g14389.t5.CDS5 | 230799 | 230846 |
| chr_4 | g14389 | g14389.t5 | exon | g14389.t5.exon6 | 230909 | 231427 |
| chr_4 | g14389 | g14389.t5 | cds | g14389.t5.CDS6 | 230909 | 231427 |
| chr_4 | g14389 | g14389.t5 | TTS | g14389.t5 | NA | NA |
Sequences
>g14389.t5 Gene=g14389 Length=1653
ATGAAACCTTATCGTCTTGTTCATCATCAGAGGTCAGTTTACAATGAAGATTTGTTGTTG
AATCCAAAAGAGCATCCAAATATCAAAAAGGGTGATATTGTAGAAATTTTTCATCAAGAT
GAAAATGATGAAAAAGGAAAACAACGCTGTCGTTTGCTTCTTCAAGTTTCACTGACAAAA
GACACTCAAGGAAGAAATGATTACATCAGTGTGGAATCTTCAATAGCTTTAACTTTTAAT
TTAAAGAACTATGGTGATGTTTTCATGCAAGTCGTTAATCCAGCTAGTGTTGCCTTAGAT
AGTGTAGAAATAACGTTCAAAGATCAATATATGGGACGATCAGAAATGTGGCGTTTAAAG
CAGCATCTTACAAAAACCTGTGTCTACATTAATAAAAAAATCGAATACTGTGATTCCATT
CGTTGTCAAGTTTATGAAATGTGGAGTTTTGGAGAAAGAGTATCATGTGGAGTTATTACA
GAAGATACAAAAGTTGTTTTTAGGAGTAGTACCTCCATGGTTTACCTATTTATGCAAATG
AGCTCTGAAATGTGGGACTTTGATATTCATGGAGACCTTTATTTTGAAAAGGCTGTAAAT
GGTTTTTTGACAGACCTTTTTGCAAAATGGAAAAAACAAGGCAGCAATCATGAAGTCACA
ATTGTGTTATTTTCACGAACATTTTATGCTGCAAAAAATCTGGAGGAATTTCCTGAACAT
ATGCGAGATTGTTTGCAAATGGATTATAAAGGAAGATTTTATGAGGATTTTTATCGTGTA
GCCATTCAAAATGAAAGAAACGATGATTGGAGCACAATTTTAGTGCAACTTAGACGTCTT
TTTACATCATACAAAGATATTGTATTGAAATATCATGAAAGACCAGGAGAAATAATACCA
ATGGCAATAAATTCAACCGCTGCCCAGGGCAATTTTCTTGAAGTTTTAAATATCTCATTG
AATGTCTTTGAAAAGCATTATCTTGATAGAAGTTTTGATCGAACTGGTCAATTATCAATT
GTAATAACACCAGGAGTTGGCGTATTCGAAGTAGATCGAGAGCTTACTAATATTACTAAG
CAGAGAATTATTGATAATGGCGTTGGAAGTGATTTAGTTTGTGTTGGTGAACAGCCATTA
CATGCAGTACCTCTTTTGAAATTTCACAACAAAGATCCTTCATTAAATTCAGTAGATGAT
TATTCAATGCCACACTGGGTTAATCTGAGTTTTTATTCTACCAATAAAAAAGTCGCTTAT
TCAACTTTCATACCACGTATTAAATTACCTCCACAAATTCGTAAAAATGAAGATGACAAT
GAAGTGTTGCATGTAAAAAAACTCAAAGAAGAAATTAATTTTTATACTGACACAAATACT
GAATATATTCATAATTCACTTTTTGATTATGATGCATATGATGCACAAGTTTTTGCATTT
CCCAGTCATACAACACTTATACAAAAAACTAAAAAATCAAGTATAGATGGTTTCAATTCA
TATTCAGTGTCAGTGACATCAAATAATGGCAGCAATGGATTTCAACGAGTCCGTAAGATG
TCTGATCCTGATATTTATCATGCTTGCAGTTTTTCAGAAACAAATGGTGCTTTACGTGAA
TCATTATCACAATCAACGTTGACAGCTACAAAA
>g14389.t5 Gene=g14389 Length=551
MKPYRLVHHQRSVYNEDLLLNPKEHPNIKKGDIVEIFHQDENDEKGKQRCRLLLQVSLTK
DTQGRNDYISVESSIALTFNLKNYGDVFMQVVNPASVALDSVEITFKDQYMGRSEMWRLK
QHLTKTCVYINKKIEYCDSIRCQVYEMWSFGERVSCGVITEDTKVVFRSSTSMVYLFMQM
SSEMWDFDIHGDLYFEKAVNGFLTDLFAKWKKQGSNHEVTIVLFSRTFYAAKNLEEFPEH
MRDCLQMDYKGRFYEDFYRVAIQNERNDDWSTILVQLRRLFTSYKDIVLKYHERPGEIIP
MAINSTAAQGNFLEVLNISLNVFEKHYLDRSFDRTGQLSIVITPGVGVFEVDRELTNITK
QRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDPSLNSVDDYSMPHWVNLSFYSTNKKVAY
STFIPRIKLPPQIRKNEDDNEVLHVKKLKEEINFYTDTNTEYIHNSLFDYDAYDAQVFAF
PSHTTLIQKTKKSSIDGFNSYSVSVTSNNGSNGFQRVRKMSDPDIYHACSFSETNGALRE
SLSQSTLTATK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g14389.t5 | PANTHER | PTHR13179 | DEP DOMAIN CONTAINING PROTEIN 5 | 4 | 525 | 0 |
| 1 | g14389.t5 | Pfam | PF12257 | Vacuolar membrane-associated protein Iml1 | 102 | 388 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005096 | GTPase activator activity | MF |
KEGG
Orthology
- This transcript has been annotated with K20404 (SEA/GATOR complex protein SEA1/DEPDC5)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed