Gene loci information

Transcript annotation

  • This transcript has been annotated as Phosphoenolpyruvate carboxykinase [GTP].

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g14398 g14398.t12 isoform g14398.t12 267446 268949
chr_4 g14398 g14398.t12 exon g14398.t12.exon1 267446 267689
chr_4 g14398 g14398.t12 cds g14398.t12.CDS1 267448 267689
chr_4 g14398 g14398.t12 exon g14398.t12.exon2 267744 268165
chr_4 g14398 g14398.t12 cds g14398.t12.CDS2 267744 268065
chr_4 g14398 g14398.t12 exon g14398.t12.exon3 268914 268949
chr_4 g14398 g14398.t12 TSS g14398.t12 269106 269106
chr_4 g14398 g14398.t12 TTS g14398.t12 NA NA

Sequences

>g14398.t12 Gene=g14398 Length=702
ATGTTTCGTGTTTTAAAAAGAGTTAGTACAATGTAAATCTTTCAATCATCATTCAACCAT
TAATGCTTCGTTTTTGAATCGTAAAATTCATTCCTATCAACTTGCAAATGGATTCGATCC
AGCCGTACTTCCATCTATGGTTCGAAATTTCATTGAAGATTCTGCTCGACTCTGTGAACC
TGATTCAATACACATTTGTGATGGAAGTGAAAGTGAAAATAAAATGATGCTAAATGAAAT
GCAAGCTTCTGGTACAATCGTATCATTACCAAAATACGATAATTGTTGGTTAGCTAAAAC
AAATCCAGCTGATGTTGCTCGTGTTGAAAGTAAAACTTTTATTTGTACTGAAATGAAAGA
AGATGCTGTTTGTATACCAAAAAAAGGAGTAACTGGTATGCTCGGAAATTGGATTAGTCC
TCAAGATTACGAAAAAGCTATTTTAGACAGATTTCCAGGTTCGATGAGAGGCAGAAAAAT
GTATGTTGTACCATTTTCAATGGGTCCATTAGGTTCAAAACTATCAAAAATTGGAATAGA
AATAACGGACTCCCCATATGTCGTCGCATCAATGCGTATTATGACTCGAATGGGTTCATC
CGTTTTAGACTTAATTGATGATGATTTTGTTCGTTGTCTTCATTCTATTGGGCGTCCTGC
AAGTGGAATCATTGAAATGCCTTCATGGATTTGTGATCCACA

>g14398.t12 Gene=g14398 Length=188
MVRNFIEDSARLCEPDSIHICDGSESENKMMLNEMQASGTIVSLPKYDNCWLAKTNPADV
ARVESKTFICTEMKEDAVCIPKKGVTGMLGNWISPQDYEKAILDRFPGSMRGRKMYVVPF
SMGPLGSKLSKIGIEITDSPYVVASMRIMTRMGSSVLDLIDDDFVRCLHSIGRPASGIIE
MPSWICDP

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g14398.t12 Gene3D G3DSA:3.40.449.10 Phosphoenolpyruvate Carboxykinase 1 188 0
2 g14398.t12 PANTHER PTHR11561 PHOSPHOENOLPYRUVATE CARBOXYKINASE 2 188 0
3 g14398.t12 PANTHER PTHR11561:SF11 PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP], MITOCHONDRIAL 2 188 0
1 g14398.t12 Pfam PF17297 Phosphoenolpyruvate carboxykinase N-terminal domain 4 188 0
4 g14398.t12 SUPERFAMILY SSF68923 PEP carboxykinase N-terminal domain 2 187 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006094 gluconeogenesis BP
GO:0005525 GTP binding MF
GO:0004611 phosphoenolpyruvate carboxykinase activity MF
GO:0017076 purine nucleotide binding MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values