Gene loci information

Transcript annotation

  • This transcript has been annotated as G protein-coupled receptor kinase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g14501 g14501.t1 isoform g14501.t1 871068 884423
chr_4 g14501 g14501.t1 exon g14501.t1.exon1 871068 871262
chr_4 g14501 g14501.t1 cds g14501.t1.CDS1 871068 871262
chr_4 g14501 g14501.t1 exon g14501.t1.exon2 871321 871474
chr_4 g14501 g14501.t1 cds g14501.t1.CDS2 871321 871474
chr_4 g14501 g14501.t1 exon g14501.t1.exon3 871535 871741
chr_4 g14501 g14501.t1 cds g14501.t1.CDS3 871535 871741
chr_4 g14501 g14501.t1 exon g14501.t1.exon4 871973 872181
chr_4 g14501 g14501.t1 cds g14501.t1.CDS4 871973 872181
chr_4 g14501 g14501.t1 exon g14501.t1.exon5 872243 872327
chr_4 g14501 g14501.t1 cds g14501.t1.CDS5 872243 872327
chr_4 g14501 g14501.t1 exon g14501.t1.exon6 872388 872612
chr_4 g14501 g14501.t1 cds g14501.t1.CDS6 872388 872612
chr_4 g14501 g14501.t1 exon g14501.t1.exon7 872667 872762
chr_4 g14501 g14501.t1 cds g14501.t1.CDS7 872667 872762
chr_4 g14501 g14501.t1 exon g14501.t1.exon8 872820 873199
chr_4 g14501 g14501.t1 cds g14501.t1.CDS8 872820 873199
chr_4 g14501 g14501.t1 exon g14501.t1.exon9 873254 873442
chr_4 g14501 g14501.t1 cds g14501.t1.CDS9 873254 873442
chr_4 g14501 g14501.t1 exon g14501.t1.exon10 873498 873673
chr_4 g14501 g14501.t1 cds g14501.t1.CDS10 873498 873673
chr_4 g14501 g14501.t1 exon g14501.t1.exon11 880721 880797
chr_4 g14501 g14501.t1 cds g14501.t1.CDS11 880721 880797
chr_4 g14501 g14501.t1 exon g14501.t1.exon12 884317 884423
chr_4 g14501 g14501.t1 cds g14501.t1.CDS12 884317 884423
chr_4 g14501 g14501.t1 TSS g14501.t1 NA NA
chr_4 g14501 g14501.t1 TTS g14501.t1 NA NA

Sequences

>g14501.t1 Gene=g14501 Length=2100
ATGGCGGATTTAGAGGCAGTATTGGCCGATGTCAGTTATCTGATGGCTATGGAGATGAGC
AAGCAAACGCCGGAGTCAAGTAAAAAAATCATTCTACCTGATACCAGCGTTCGAAGTGCA
ATGCATAAATTTCTTGAACGTGAAAATGAAGTGACATTTGATAAAATTTTCAATCAAAAA
ATTGGATTTCTCTTATTCAAAGACTATTGTGAAGGTTACCGTGATGAGCCAGTTCCACAA
TTGAAATTTTATGAAGAAATTCGCAATTTTGAAAAAACTGAATGTACAGAAGAGCGTAAA
AAATTGGCCAAATCAATTTATGATAATTTTATCATGAAAGAAATGTTGAGTCATACACAT
GAATATTCCAAAGAGTGCGTAGCACATGTCAATAGTTATTTACAAAAAAATGAAGTTCCC
ACAAATCTTTTTGAGCCATATATTGAAGAGATTTTCAATCATTTGCGGGGTGATATTTTC
AGAGGTTTTATGGAAAGCTCACGTTACACGAGATTTTTACAATGGAAAAATTTGGAGCTT
AATTTGAAGCTAACAATGAATGACTTTAGTGTGCTTCGAATTATTGGTCGTGGTGGTTTT
GGTGAAGTTTACGGTTGCCGTAAAGTTGATACTGGTAAAATGTACGCAATGAAATGTCTC
GATAAGAAACGTATTAAAATGAAGCAAGGTGAAACGCTGGCACTAAATGAGCGTACCATG
TTAAGTCTAGTATCAACTGGTGAAGACTGTCCATTTATTGTATGCATGACATATGCATTT
CATACGGCCAGCAAACTTTGTTTTATCCTTGACCTCATGAATGGTGGCGATCTTCATTAT
CATTTGTCACAACATGGTGTCTTCAATGAAACAAACATGAAATTTTATGCGACTGAAATT
ATTCTTGGTCTTGAACATATGCATAAAAGGTACATTGTTTATAGAGATCTAAAGCCTGCC
AATATTTTACTTGATGAGAATGGTCACGTGCGGATAAGTGATCTTGGTCTTGCGTGTGAT
TTTAGTAAAAAGAAGCCACATGCATCAGTCGGAACTCACGGGTATATGGCACCAGAAGTA
CTATGCAAAGGTATGGGATATGATAGTTGTGCCGATTGGTTTAGTTTTGGTTGCATGATT
TATAAGCTTCTGAAGGGTCATAGTCCATTTAGACAGCATAAAACAAAAGATAAACATGAA
ATTGACAGAATGACATTGACAATGAAAGTTGAATTACCGGATTCTTACAGTTCCGAGGTG
AAAAGTTTACTTGATGGACTTTTACAGCGAGAAATTGATAAAAGATTGGGTTGTGGTGGT
CAAGGTGCTGAAGAGGTCAAAAATCATCCATTTTTCCATGGTGTTGATTGGAAGCAAGTA
TATCAACAAAATTATGCACCACCATTTATACCACCAAAAGGAGAAGTCAATGCAGCAGAT
GCTTGTGATATTGGCAATTTTGATGAAGACGACACCAAAGCTGTCAAAATTACAGAACAG
GATCAGGAAATTTATCGTGATTTTTCACTTACTATCAGTGAAAGATGGCAACAAGAAATA
ACCGAAACAGTATTTGAATCAGTAAATCAAGAAGCTGATAAGCTTGAAAAGAAAAATAAA
GCAAAGAAAACTGCAAAATTCTTCGATGACGAAAAAGAGAGTGATTGCATACTGCAAGGT
TACATTAAAAAACGTGGTAATTCATTCGCATCAGGATGGCAAACGAAATATGCCAAGTTA
TATCCAAATAGACTGGAACTTTTGCCACCAAGTCGTAACAACAAACCAGAGCTGATTTTT
ATGGATCAGATTGATGAAGTCGCAGCCGATGTGCCATATAAAAATGAGAATTGCATTCAA
ATTAAATTAAAAGACAATATCACGCGCATCATTTTACACAATCAGGATCAAATAGCGCAA
AAAGAGTGGATCCAATCAATACGCTTGGCTCATAAAACGAGTCAAGAATTGCTTGGTTCA
ATAATAAATAAAAAAGTTGGCAAAATTTATGGCACTGAAATTGATGTTAGTAGCAAGTCT
GTCACAATTACAGCATCATCAAACAGCATCACGACAAAAAGCACAAATGGTAGCAACTAG

>g14501.t1 Gene=g14501 Length=699
MADLEAVLADVSYLMAMEMSKQTPESSKKIILPDTSVRSAMHKFLERENEVTFDKIFNQK
IGFLLFKDYCEGYRDEPVPQLKFYEEIRNFEKTECTEERKKLAKSIYDNFIMKEMLSHTH
EYSKECVAHVNSYLQKNEVPTNLFEPYIEEIFNHLRGDIFRGFMESSRYTRFLQWKNLEL
NLKLTMNDFSVLRIIGRGGFGEVYGCRKVDTGKMYAMKCLDKKRIKMKQGETLALNERTM
LSLVSTGEDCPFIVCMTYAFHTASKLCFILDLMNGGDLHYHLSQHGVFNETNMKFYATEI
ILGLEHMHKRYIVYRDLKPANILLDENGHVRISDLGLACDFSKKKPHASVGTHGYMAPEV
LCKGMGYDSCADWFSFGCMIYKLLKGHSPFRQHKTKDKHEIDRMTLTMKVELPDSYSSEV
KSLLDGLLQREIDKRLGCGGQGAEEVKNHPFFHGVDWKQVYQQNYAPPFIPPKGEVNAAD
ACDIGNFDEDDTKAVKITEQDQEIYRDFSLTISERWQQEITETVFESVNQEADKLEKKNK
AKKTAKFFDDEKESDCILQGYIKKRGNSFASGWQTKYAKLYPNRLELLPPSRNNKPELIF
MDQIDEVAADVPYKNENCIQIKLKDNITRIILHNQDQIAQKEWIQSIRLAHKTSQELLGS
IINKKVGKIYGTEIDVSSKSVTITASSNSITTKSTNGSN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
18 g14501.t1 Coils Coil Coil 525 545 -
17 g14501.t1 Gene3D G3DSA:1.20.58.1850 - 29 524 6.4E-200
14 g14501.t1 Gene3D G3DSA:3.30.200.20 Phosphorylase Kinase; domain 1 181 276 6.4E-200
16 g14501.t1 Gene3D G3DSA:1.10.510.10 Transferase(Phosphotransferase) domain 1 277 472 6.4E-200
15 g14501.t1 Gene3D G3DSA:2.30.29.30 - 552 660 1.7E-14
4 g14501.t1 PANTHER PTHR24355:SF18 G PROTEIN-COUPLED RECEPTOR KINASE 1 675 0.0
5 g14501.t1 PANTHER PTHR24355 G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE 1 675 0.0
10 g14501.t1 PRINTS PR00717 GPCR kinase signature 169 181 2.1E-20
9 g14501.t1 PRINTS PR00717 GPCR kinase signature 228 246 2.1E-20
8 g14501.t1 PRINTS PR00717 GPCR kinase signature 467 484 2.1E-20
7 g14501.t1 PRINTS PR00717 GPCR kinase signature 492 505 2.1E-20
6 g14501.t1 PRINTS PR00717 GPCR kinase signature 506 523 2.1E-20
1 g14501.t1 Pfam PF00615 Regulator of G protein signaling domain 53 172 1.4E-19
3 g14501.t1 Pfam PF00069 Protein kinase domain 190 452 2.1E-69
2 g14501.t1 Pfam PF00169 PH domain 558 650 1.5E-6
20 g14501.t1 ProSitePatterns PS00107 Protein kinases ATP-binding region signature. 195 218 -
19 g14501.t1 ProSitePatterns PS00108 Serine/Threonine protein kinases active-site signature. 312 324 -
25 g14501.t1 ProSiteProfiles PS50132 RGS domain profile. 52 173 39.76
27 g14501.t1 ProSiteProfiles PS50011 Protein kinase domain profile. 189 452 44.612
26 g14501.t1 ProSiteProfiles PS51285 AGC-kinase C-terminal domain profile. 453 520 11.734
28 g14501.t1 ProSiteProfiles PS50003 PH domain profile. 555 652 10.419
21 g14501.t1 SMART SM00315 RGS_3 52 173 2.7E-31
24 g14501.t1 SMART SM00220 serkin_6 189 452 2.8E-87
22 g14501.t1 SMART SM00133 pkinase_C_6 453 532 8.9E-11
23 g14501.t1 SMART SM00233 PH_update 556 654 3.9E-9
13 g14501.t1 SUPERFAMILY SSF48097 Regulator of G-protein signaling, RGS 28 181 1.43E-42
12 g14501.t1 SUPERFAMILY SSF56112 Protein kinase-like (PK-like) 185 525 2.87E-84
11 g14501.t1 SUPERFAMILY SSF50729 PH domain-like 551 659 1.77E-16

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004703 G protein-coupled receptor kinase activity MF
GO:0007165 signal transduction BP
GO:0005524 ATP binding MF
GO:0004674 protein serine/threonine kinase activity MF
GO:0004672 protein kinase activity MF
GO:0006468 protein phosphorylation BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values