General information for g14540.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Aspartate aminotransferase, mitochondrial.
Parent gene
- The parent gene g14540 is located on chr_4: 1152660..1152966 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14540 | g14540.t1 | TSS | g14540.t1 | 1150000 | 1150000 |
| chr_4 | g14540 | g14540.t1 | isoform | g14540.t1 | 1150070 | 1152966 |
| chr_4 | g14540 | g14540.t1 | exon | g14540.t1.exon1 | 1150070 | 1150134 |
| chr_4 | g14540 | g14540.t1 | cds | g14540.t1.CDS1 | 1150070 | 1150134 |
| chr_4 | g14540 | g14540.t1 | exon | g14540.t1.exon2 | 1150198 | 1150489 |
| chr_4 | g14540 | g14540.t1 | cds | g14540.t1.CDS2 | 1150198 | 1150489 |
| chr_4 | g14540 | g14540.t1 | exon | g14540.t1.exon3 | 1152064 | 1152966 |
| chr_4 | g14540 | g14540.t1 | cds | g14540.t1.CDS3 | 1152064 | 1152966 |
| chr_4 | g14540 | g14540.t1 | TTS | g14540.t1 | 1153159 | 1153159 |
Sequences
>g14540.t1 Gene=g14540 Length=1260
ATGGTTCGATTGACGTCATTATTGCGTGATCCATTAAAATTGAAACAAGCTGTTCCACCA
GCTGTTCAATCATGGTGGAACAACGTACCGCTCGGTCCACCTGATGTCATTCTCGGCATC
TATGAAGCCCATAAAGTTGATCCAAATCCAAATAAAGTAGATTTAACTGTCGGTGCATAT
CGTGATGAAAATGGCAAACCTTACGTTCTTAAAACAATTTTAAAAGCAGAGCAAAAAATT
GTCGATAAATGTTTAGATAAAGAATATAATGATATTGGCAGTGAATATTTTCGAGAGGTG
ACGTATCGTCTCGCCGTTGGAAAAGAGCTTAGCGATCGTGCACATGTTAGTGTTCAAAGC
ATCAGTGGAAGTGGCAGCATAAAACTCGCAGCTGAAACCATAAGTCGTCTATATAAAGGC
AATAAACTAATGTTCATACCAAATCCATCGTGGGCATATCATGCACCAATGTTTGAACTC
TCGGGCATGAATACAGCATTTTATCGTTATTATGACGATCGAACACATGAAATTGACCAC
AGTGGTCTCATGACAGATCTTATGCACATGCCTGATAATGCAATGGTTCTTTTCCAAATG
GTTGGTCACAATCCAACAGCAACAGATCCAACAAATGAACAATGGCGTGAAATGTCAAAA
ATTTTAAAAAATAAAAATGTTTTGGTATTTTTTGACATGGCATATCAAGGTTTTGCAAGT
GGTTGTGTTGACACAGATGCATTTGCAGTTCGACATTTTATTGAAGAAGGACATAAAGTT
GTATTTGCACAGAGTTATAGTAAAAATATGGGAATGTATAGTGTAAGAGTTGGTGCAACG
ACATTTATGGTTGATAAAAAAGAAGAAGCAGAATCATTAATGGAAGTTATGAAGCATCTT
GCAATGTGCTGTTATGGTCAACCACCAATTCATGGTTCACAAGTTGTTGAGGAAATTTTT
AGAGATGAAAAACTTCATCAAGAATGGAAAGATGAATTGAAAATGATGACTGGAAGAATC
CATAAAATGCGACATTTGTTAAAGAGAAAATTAGAAGAAATCGGATCAAGTCACGATTGG
AGTCATGTGTTAAAACAGCAAGGAATGTTTTTCTATAGTGGATTAAGTGTTGAGCAATGT
GAAAGTCTGATAAGAGATCATAGTATTTATGTTGTGAAAAATGGTCGAATGGCAATACCT
GGAATCAATGAGAAAAATGTTGATTATGTTGTAAAATGCTTACATGAAGTTACAAAATAA
>g14540.t1 Gene=g14540 Length=419
MVRLTSLLRDPLKLKQAVPPAVQSWWNNVPLGPPDVILGIYEAHKVDPNPNKVDLTVGAY
RDENGKPYVLKTILKAEQKIVDKCLDKEYNDIGSEYFREVTYRLAVGKELSDRAHVSVQS
ISGSGSIKLAAETISRLYKGNKLMFIPNPSWAYHAPMFELSGMNTAFYRYYDDRTHEIDH
SGLMTDLMHMPDNAMVLFQMVGHNPTATDPTNEQWREMSKILKNKNVLVFFDMAYQGFAS
GCVDTDAFAVRHFIEEGHKVVFAQSYSKNMGMYSVRVGATTFMVDKKEEAESLMEVMKHL
AMCCYGQPPIHGSQVVEEIFRDEKLHQEWKDELKMMTGRIHKMRHLLKRKLEEIGSSHDW
SHVLKQQGMFFYSGLSVEQCESLIRDHSIYVVKNGRMAIPGINEKNVDYVVKCLHEVTK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g14540.t1 | CDD | cd00609 | AAT_like | 53 | 416 | 0 |
| 9 | g14540.t1 | Gene3D | G3DSA:3.90.1150.10 | Aspartate Aminotransferase | 35 | 415 | 0 |
| 10 | g14540.t1 | Gene3D | G3DSA:3.40.640.10 | - | 69 | 337 | 0 |
| 2 | g14540.t1 | PANTHER | PTHR11879 | ASPARTATE AMINOTRANSFERASE | 22 | 419 | 0 |
| 3 | g14540.t1 | PANTHER | PTHR11879:SF22 | ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL | 22 | 419 | 0 |
| 7 | g14540.t1 | PRINTS | PR00799 | Aspartate aminotransferase signature | 196 | 215 | 0 |
| 6 | g14540.t1 | PRINTS | PR00799 | Aspartate aminotransferase signature | 227 | 239 | 0 |
| 4 | g14540.t1 | PRINTS | PR00799 | Aspartate aminotransferase signature | 295 | 320 | 0 |
| 5 | g14540.t1 | PRINTS | PR00799 | Aspartate aminotransferase signature | 363 | 381 | 0 |
| 1 | g14540.t1 | Pfam | PF00155 | Aminotransferase class I and II | 51 | 412 | 0 |
| 8 | g14540.t1 | SUPERFAMILY | SSF53383 | PLP-dependent transferases | 23 | 418 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0009058 | biosynthetic process | BP |
| GO:0006520 | cellular amino acid metabolic process | BP |
| GO:0008483 | transaminase activity | MF |
| GO:0003824 | catalytic activity | MF |
| GO:0030170 | pyridoxal phosphate binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed