General information for g14833.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Scoloptoxin SSD14.
Parent gene
- The parent gene g14833 is located on chr_4: 2590456..2592327 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14833 | g14833.t1 | isoform | g14833.t1 | 2590456 | 2592327 |
| chr_4 | g14833 | g14833.t1 | exon | g14833.t1.exon1 | 2590456 | 2590562 |
| chr_4 | g14833 | g14833.t1 | cds | g14833.t1.CDS1 | 2590456 | 2590562 |
| chr_4 | g14833 | g14833.t1 | exon | g14833.t1.exon2 | 2590621 | 2591292 |
| chr_4 | g14833 | g14833.t1 | cds | g14833.t1.CDS2 | 2590621 | 2591292 |
| chr_4 | g14833 | g14833.t1 | exon | g14833.t1.exon3 | 2591397 | 2592327 |
| chr_4 | g14833 | g14833.t1 | cds | g14833.t1.CDS3 | 2591397 | 2592327 |
| chr_4 | g14833 | g14833.t1 | TTS | g14833.t1 | 2592402 | 2592402 |
| chr_4 | g14833 | g14833.t1 | TSS | g14833.t1 | NA | NA |
Sequences
>g14833.t1 Gene=g14833 Length=1710
ATGAAGACTCCAGTACCGAAAAAGTTTATTATAACAGTAATTTTGTTGTTATGTTCAACA
AATTTCAGAACAATTGACGCTTCCGCTTTTCCTTCTAATATTCTTACGTTTTATAAGGCA
GCAATTGTCACAAATGGCCTTGAATGTGCATCACTAGGCAAAAAAATATTTGATCTGGGT
GGAAATGTTGCTGATGTTGCTGTGACTGTTGTTTTATGTGAAGGCGTGGCAAATCCACAA
AGTTCAGGCATTGGTGGTGGATTTTTGCTCAGTTATTATTCGAAATCTGAAGGAATCGTG
AGAACTTTGAATGCTCGTGAAACTGCACCTGCTGCTGCAACGACTGATATGTATATTGGT
AAACCAAATCTTTCAGTTTCTGGTGGAAAAGCAGTTGCTGTTCCGGGTGAAATTAAAGGA
CTTTGGGAATTACATCAAAAATTTGGAAGTTTAGAATGGAAAACTTTGTTGCAACCTGTG
ATTGAATTATGTAAAGAAGGTCAAGTTGTGTCAAACTATTTGCGAAATGTTTTTGGTCGT
TATGAAGATAGAATTTTCAATGAACCAAGTTTGAAAGAAATTTTTATTGATCCTTCGACC
AATCATACTTATAAATTAGGAGAAAAAGTTAAAAGATTGAAACTTGCGGAGACACTCGAA
TCTATAGCAGAAAAAGGAGCGAATGTAATTTATGGAGGTGGTGAAATCGGAAGAAAGTTG
ATTGAAGATGTTCAGAAAATGAATGGAATTTTAACAGAAGAAGATTTGATGAGTTATAAA
GTAAAATGGTCAACATCATTAACATCAAAAATAATCAACAATCAAACCCTGCATACTTTT
CCAATTCCAAGCAGTGGTGCAATTTTAAATTTCATTTTTAATGTCCTAAGCAATTATAAA
CTTTCTCACGATTCTCTAAGTTATCACAGAATTATTGAAGCTTTTAAATTTGCTTATGCA
AAACGAAGTTATTTAGGTGATGAAAATTCAACTGAAGTTCTTGAAATGATGGAAAATTTA
TTAAGTCAAAATTATGCAAATGAAATTTCTTCTATGATTAATGATACTAAAACTTTCAAT
GATCCTAAATATTACAAACCATTTTTTGAAATCATTGAAGATAAGGGCACAACTCACATG
TCAATTTTAGCTCCAAACGGTGATGCAATTTCAGTCACGAGCACAATAAATTATTATTTT
GGTGCTTTCTTTAGATCTGTACAAACAGGAATAATTTTGAATAACGAAATGGATGATTTT
TCTACTCCAGGACTTTTGAATATTTATGGATATTTACCATCGCCTACTAACTTTATTAAA
CCTGGAAAACATCCTCAGAGTTCGATGACTCCGACTTTCATAATTGATGAAAAAGGAAAT
ATTCGAATGATCGTTGGTGCTTCAGGAGGATCGAGAATTCCGACTGGCATTTTTTCAACT
ATTTTCAATCATCTTTATCTTAAAAAATCTTTAAAAGATGCAATTGGAACAAGAAGACTT
CATCATCAATTGTTTCCGCCACAATTGCAATATGAAACTGGTTTTGATAGCGAAATAATT
AAGGAATTGAATGAAAAATTTGGTCATTTATTATTAGAAAATACACAAAATGATAATTTT
GCTGCAGTAACTGGAATTGCTATAAACAATGGAAAAATCGAAGCAAGTTTTGATCCTAGA
CGTGGTGGAAGTGTTAAAATTTTTAAATAG
>g14833.t1 Gene=g14833 Length=569
MKTPVPKKFIITVILLLCSTNFRTIDASAFPSNILTFYKAAIVTNGLECASLGKKIFDLG
GNVADVAVTVVLCEGVANPQSSGIGGGFLLSYYSKSEGIVRTLNARETAPAAATTDMYIG
KPNLSVSGGKAVAVPGEIKGLWELHQKFGSLEWKTLLQPVIELCKEGQVVSNYLRNVFGR
YEDRIFNEPSLKEIFIDPSTNHTYKLGEKVKRLKLAETLESIAEKGANVIYGGGEIGRKL
IEDVQKMNGILTEEDLMSYKVKWSTSLTSKIINNQTLHTFPIPSSGAILNFIFNVLSNYK
LSHDSLSYHRIIEAFKFAYAKRSYLGDENSTEVLEMMENLLSQNYANEISSMINDTKTFN
DPKYYKPFFEIIEDKGTTHMSILAPNGDAISVTSTINYYFGAFFRSVQTGIILNNEMDDF
STPGLLNIYGYLPSPTNFIKPGKHPQSSMTPTFIIDEKGNIRMIVGASGGSRIPTGIFST
IFNHLYLKKSLKDAIGTRRLHHQLFPPQLQYETGFDSEIIKELNEKFGHLLLENTQNDNF
AAVTGIAINNGKIEASFDPRRGGSVKIFK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g14833.t1 | Gene3D | G3DSA:1.10.246.130 | - | 267 | 369 | 5.2E-31 |
| 16 | g14833.t1 | Gene3D | G3DSA:3.60.20.40 | - | 372 | 568 | 8.5E-65 |
| 2 | g14833.t1 | PANTHER | PTHR11686 | GAMMA GLUTAMYL TRANSPEPTIDASE | 25 | 565 | 2.8E-209 |
| 3 | g14833.t1 | PANTHER | PTHR11686:SF51 | GAMMA-GLUTAMYL TRANSPEPTIDASE, ISOFORM A | 25 | 565 | 2.8E-209 |
| 12 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 62 | 87 | 3.6E-65 |
| 5 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 131 | 149 | 3.6E-65 |
| 10 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 149 | 168 | 3.6E-65 |
| 9 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 250 | 266 | 3.6E-65 |
| 8 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 280 | 299 | 3.6E-65 |
| 6 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 377 | 395 | 3.6E-65 |
| 7 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 401 | 419 | 3.6E-65 |
| 4 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 441 | 456 | 3.6E-65 |
| 11 | g14833.t1 | PRINTS | PR01210 | Gamma-glutamyltranspeptidase signature | 466 | 483 | 3.6E-65 |
| 1 | g14833.t1 | Pfam | PF01019 | Gamma-glutamyltranspeptidase | 55 | 563 | 2.9E-161 |
| 19 | g14833.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 27 | - |
| 20 | g14833.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 8 | - |
| 21 | g14833.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 9 | 19 | - |
| 22 | g14833.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 20 | 27 | - |
| 18 | g14833.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 28 | 569 | - |
| 13 | g14833.t1 | SUPERFAMILY | SSF56235 | N-terminal nucleophile aminohydrolases (Ntn hydrolases) | 33 | 565 | 2.12E-144 |
| 15 | g14833.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 27 | - |
| 23 | g14833.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 24 | - |
| 14 | g14833.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 27 | - |
| 24 | g14833.t1 | TIGRFAM | TIGR00066 | g_glut_trans: gamma-glutamyltransferase | 48 | 560 | 7.9E-112 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006751 | glutathione catabolic process | BP |
| GO:0036374 | glutathione hydrolase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K18592 (gamma-glutamyltranspeptidase / glutathione hydrolase / leukotriene-C4 hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed