General information for g14930.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Kynureninase.
Parent gene
- The parent gene g14930 is located on chr_4: 3094974..3100046 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g14930 | g14930.t5 | TTS | g14930.t5 | 3094911 | 3094911 |
| chr_4 | g14930 | g14930.t5 | isoform | g14930.t5 | 3094974 | 3096378 |
| chr_4 | g14930 | g14930.t5 | exon | g14930.t5.exon1 | 3094974 | 3096226 |
| chr_4 | g14930 | g14930.t5 | cds | g14930.t5.CDS1 | 3094974 | 3096226 |
| chr_4 | g14930 | g14930.t5 | exon | g14930.t5.exon2 | 3096285 | 3096378 |
| chr_4 | g14930 | g14930.t5 | cds | g14930.t5.CDS2 | 3096285 | 3096348 |
| chr_4 | g14930 | g14930.t5 | TSS | g14930.t5 | 3096378 | 3096378 |
Sequences
>g14930.t5 Gene=g14930 Length=1347
AGTAGACAAATTGTCGTTAATTTGATCACAATGAGCGAGAACTTCAATTTAAATTCATTA
AGAAATAATTTACAAGAAAAAGAAGAACAAAAAACTACAAAGAAAAATTTAAAAATTGAA
AACTTCACAACACGTCAAGATTTTGAAAAAGCAGATGAAATTGATGAATTGAAAGATTTT
CGAGAAAAGTTTCAACTTCCATCTGATGTGATTTATTTCATTGGAAATTCACTCGGCCCA
TGTGCAGTAAACACTCAAGAAATCATCAGTAAATTCATCACTGACAATTGGATAAAAAAT
TCAAGTTCAGCTGAATTTCATCAAACACCATTAAAAGTTGGTGAAAAAATTGGAAAAATT
ATTGGTGCTGAAAAGGATGAAACAATTGTTTGTGATTCAACTTCTGTCAATATTTTTAAA
GCACTTGGAACAGCTTTGAAAATTCAAAAACTCAAAAATCCAAACAGACGTGTGATTTTG
CTTGAAAAACAAAATTTTCCATCAGATAATTACATTGTGCAAGGTCTTGTAAATTTTCTA
TCAGCTGAAAATTATAAAATTGAAAAGTTTGAAGATGAGAAAGAACTTGAAAAAATTTTA
AGTACTGAAATTACAGTTTTATTGTTATCACATGTCAGTTATCGCACTGGAAAATTGTTT
GATATGAAAATTATCACAAAAATGGCACATGATTTTGGTGTTCTTATAATTTGGGATTTA
TGTCATTCAGTTGGTGCTGTACCAATTAATTTAACTGAATCAAATGTTGATTTTGCAGTT
GGTTGTACTTATAAATACTTAAATTGTGGTCCAGGAGCTCCTGCATTTTTTTATGTCAAT
AAAAGGCATCAAAATGTTGTTTGGCAGCCATTAGCAGGCTGGTATGGACATGAAGAACCT
TTTAAAATGAAATCAAATTATGAACCTGCAAGAAATATTCAACAATTTTTAACAGGAACT
GGGGAAATTTTTCATATGTCAATTGTTGAAAACAGCATTGAAATTTTACTTAAAGCAGAC
ATAAAAAAGATGAGAAAAAAGTCATTACTTTTGGGTGATCTTTTCATAAATCTTTTAAAT
GAAAAATGTTCAAGTTTAAAACTTTTAACACCATTAGAGCATGAAAAACGTGGAAGTCAT
TTGAGTTTTGAACATGAAAATGCTTACAATATCAGTGTGATGATTAATAAGCGTGGAATT
TGTGGAAATTTCCGTTTTCCTAATATTTTACGATTTGCTATGACTTCTTTATATTTGAGA
TTTGTTGACATTTGGGATACAGTTGAAATTATTTCTGATATTGTTAATAATTTTGAGATT
TATAAAAATCTTGCTGAAAAGTTGTGA
>g14930.t5 Gene=g14930 Length=438
MSENFNLNSLRNNLQEKEEQKTTKKNLKIENFTTRQDFEKADEIDELKDFREKFQLPSDV
IYFIGNSLGPCAVNTQEIISKFITDNWIKNSSSAEFHQTPLKVGEKIGKIIGAEKDETIV
CDSTSVNIFKALGTALKIQKLKNPNRRVILLEKQNFPSDNYIVQGLVNFLSAENYKIEKF
EDEKELEKILSTEITVLLLSHVSYRTGKLFDMKIITKMAHDFGVLIIWDLCHSVGAVPIN
LTESNVDFAVGCTYKYLNCGPGAPAFFYVNKRHQNVVWQPLAGWYGHEEPFKMKSNYEPA
RNIQQFLTGTGEIFHMSIVENSIEILLKADIKKMRKKSLLLGDLFINLLNEKCSSLKLLT
PLEHEKRGSHLSFEHENAYNISVMINKRGICGNFRFPNILRFAMTSLYLRFVDIWDTVEI
ISDIVNNFEIYKNLAEKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g14930.t5 | Coils | Coil | Coil | 7 | 30 | - |
| 5 | g14930.t5 | Gene3D | G3DSA:3.90.1150.10 | Aspartate Aminotransferase | 42 | 424 | 1.8E-132 |
| 6 | g14930.t5 | Gene3D | G3DSA:3.40.640.10 | - | 72 | 331 | 1.8E-132 |
| 9 | g14930.t5 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 25 | - |
| 2 | g14930.t5 | PANTHER | PTHR14084 | KYNURENINASE | 16 | 427 | 9.1E-97 |
| 3 | g14930.t5 | PANTHER | PTHR14084:SF0 | KYNURENINASE | 16 | 427 | 9.1E-97 |
| 8 | g14930.t5 | PIRSF | PIRSF038800 | KYNU | 30 | 432 | 1.1E-127 |
| 1 | g14930.t5 | Pfam | PF00266 | Aminotransferase class-V | 93 | 390 | 6.6E-18 |
| 4 | g14930.t5 | SUPERFAMILY | SSF53383 | PLP-dependent transferases | 34 | 428 | 1.09E-67 |
| 10 | g14930.t5 | TIGRFAM | TIGR01814 | kynureninase: kynureninase | 40 | 425 | 7.5E-90 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0030429 | kynureninase activity | MF |
| GO:0006569 | tryptophan catabolic process | BP |
| GO:0003824 | catalytic activity | MF |
| GO:0030170 | pyridoxal phosphate binding | MF |
| GO:0005737 | cytoplasm | CC |
| GO:0009435 | NAD biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01556 (kynureninase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed