General information for g15028.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Diacylglycerol O-acyltransferase 1.
Parent gene
- The parent gene g15028 is located on chr_4: 3588891..3590717 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15028 | g15028.t10 | TTS | g15028.t10 | 3587251 | 3587251 |
| chr_4 | g15028 | g15028.t10 | isoform | g15028.t10 | 3587302 | 3590717 |
| chr_4 | g15028 | g15028.t10 | exon | g15028.t10.exon1 | 3587302 | 3587487 |
| chr_4 | g15028 | g15028.t10 | cds | g15028.t10.CDS1 | 3587302 | 3587487 |
| chr_4 | g15028 | g15028.t10 | exon | g15028.t10.exon2 | 3587551 | 3587768 |
| chr_4 | g15028 | g15028.t10 | cds | g15028.t10.CDS2 | 3587551 | 3587768 |
| chr_4 | g15028 | g15028.t10 | exon | g15028.t10.exon3 | 3587838 | 3588375 |
| chr_4 | g15028 | g15028.t10 | cds | g15028.t10.CDS3 | 3587838 | 3588375 |
| chr_4 | g15028 | g15028.t10 | exon | g15028.t10.exon4 | 3588438 | 3588611 |
| chr_4 | g15028 | g15028.t10 | cds | g15028.t10.CDS4 | 3588438 | 3588611 |
| chr_4 | g15028 | g15028.t10 | exon | g15028.t10.exon5 | 3588703 | 3588752 |
| chr_4 | g15028 | g15028.t10 | cds | g15028.t10.CDS5 | 3588703 | 3588752 |
| chr_4 | g15028 | g15028.t10 | exon | g15028.t10.exon6 | 3588818 | 3589041 |
| chr_4 | g15028 | g15028.t10 | cds | g15028.t10.CDS6 | 3588818 | 3589041 |
| chr_4 | g15028 | g15028.t10 | exon | g15028.t10.exon7 | 3589409 | 3589551 |
| chr_4 | g15028 | g15028.t10 | cds | g15028.t10.CDS7 | 3589409 | 3589536 |
| chr_4 | g15028 | g15028.t10 | exon | g15028.t10.exon8 | 3590541 | 3590717 |
| chr_4 | g15028 | g15028.t10 | TSS | g15028.t10 | 3590713 | 3590713 |
Sequences
>g15028.t10 Gene=g15028 Length=1710
AATTAGTTCTATGTGAAACTTCCGAGTGTAAAGTTGTTGATTATTTGGTTTCCTCGCGTG
TTCGTCTTTTATTTATTTCTAATCTATTTTATTTATTTAAATTTTGTAAAAGTTATTATT
TGGTTCAAAATAAATAGTCTTTGTTAAGTGTGCGTAATTGTGAAAAAAGTGAACAAGTTT
AATAGAAATATTATGGCACCGTCTGAGAGCAATTGTAATGGTGAATTAAAAACGCGTCTG
AGACGAACACAAAGCGTAACACGTGCTGAAGAGATCACTGATCAAGAGCAAAAGCAAAGA
AAGAGTCAACCAGATAAACCAGTACACAAGCCACGAGATTCTCTTTTTTCGTGGAGTTCA
AATTTCAATGATTTTAGTGGTTTGGTGAATTGGGGATTTCTTTTATTGACAATGGGAGGC
ATCAGATTGCTACTAGAAAATTTTATAAAATATGGATTTCGAGTTGATCCAGTACAATGG
CTAATTGTACTGAGTGGAAAAAATGAAGGAGATTCATCTTATCCATCGGTTGTTCTAGCT
TTATATTCAAGTGTGCCAATTGTTTTATGTATTATTATTGAAAAAGGACTTTCTGAGATT
ATTCCAGAAACAACAGGCAAATTTGCACACGTGTTTAACTTGATTGCAGTAGTTCTTTTG
CCAATCGTTGTAATTAATTTACGTGGAACTTCATTTAGTTTGATGGGGTCAACATTTGTC
TGTTTTTTGTACTGTATACTTTTTGTAAAATTATGGAGTTATGTGCAGGTCAATTTATGG
TGTCGTCAAAATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGAAAGTTTT
AGTATAGCAGAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAATGAAAAT
TTTATTGCTAATGATACAAAAGACCATGAACCACTGAAATTGATAGAGTATCCTGATAAT
TTAAATCTTCGTGATCTTATCTATTTTCTTCTTGCGCCAACATTATGTTATGAATTAAAT
TTCCCAAGAACAACTCGAATTCGTAAACGGTTTTTAATCAAAAGAATTTTAGAAGTAGTT
GTCGGAGTTAATATTGTCATGGCACTTTTTCAACAATGGATGATACCAAGTGTTAAAAAT
TCTCTTATTCCTTTCTCAAATATGGAATTTACAAAAGCAACTGAACGTGTATTGAAGCTT
GCTATTCCAAATCATTTTATATGGCTGTGTGGGTTCTATCTAATTTTTCATTCAACCCTT
AATCTTCTTGGTGAAATTTTACATTTTGCTGATCGCAATTTTTACTGTGACTGGTGGAAC
GCAAATAACGTTGATACATTTTGGAAAACATGGAATATGCCTGTCCATCGTTGGTGTGTG
AGACATTTATATATTCCACTTGTTGATATGGGATATAGTCGTTTTGCTGCTTCTGGAGTT
GTTTTTTTCATTAGTGCCTTCTTCCACGAATATTTGGTATCAGTTCCTCTTCGAACATTT
AAAATTTGGGCTTTTATGGGAATGGTTTCGCAGTTACCTCTTTCTTATGTATCAAAATTT
CTCGAAAAAAATGTAGGAGAAAGATGGGGAAATATTTTAGTATGGGGAAGTTTAATTTTA
GGGCAACCACTGTGTATCATGGTCTATTATCATGACTATGTCATCCAAAATTATAATAAA
GTTATACTTGAACCTGAATATTTAGAATAG
>g15028.t10 Gene=g15028 Length=505
MAPSESNCNGELKTRLRRTQSVTRAEEITDQEQKQRKSQPDKPVHKPRDSLFSWSSNFND
FSGLVNWGFLLLTMGGIRLLLENFIKYGFRVDPVQWLIVLSGKNEGDSSYPSVVLALYSS
VPIVLCIIIEKGLSEIIPETTGKFAHVFNLIAVVLLPIVVINLRGTSFSLMGSTFVCFLY
CILFVKLWSYVQVNLWCRQNQKNQKVSISGKRRESFSIAEFERKHHRNSNTDQNENFIAN
DTKDHEPLKLIEYPDNLNLRDLIYFLLAPTLCYELNFPRTTRIRKRFLIKRILEVVVGVN
IVMALFQQWMIPSVKNSLIPFSNMEFTKATERVLKLAIPNHFIWLCGFYLIFHSTLNLLG
EILHFADRNFYCDWWNANNVDTFWKTWNMPVHRWCVRHLYIPLVDMGYSRFAASGVVFFI
SAFFHEYLVSVPLRTFKIWAFMGMVSQLPLSYVSKFLEKNVGERWGNILVWGSLILGQPL
CIMVYYHDYVIQNYNKVILEPEYLE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g15028.t10 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 16 | - |
| 12 | g15028.t10 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 46 | - |
| 13 | g15028.t10 | MobiDBLite | mobidb-lite | consensus disorder prediction | 17 | 46 | - |
| 2 | g15028.t10 | PANTHER | PTHR10408:SF7 | DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 17 | 493 | 2.3E-152 |
| 3 | g15028.t10 | PANTHER | PTHR10408 | STEROL O-ACYLTRANSFERASE | 17 | 493 | 2.3E-152 |
| 35 | g15028.t10 | PIRSF | PIRSF000439 | Oat_ACAT_DAG_ARE | 2 | 496 | 1.6E-138 |
| 36 | g15028.t10 | PIRSF | PIRSF500231 | Oat_dag | 2 | 497 | 2.9E-173 |
| 1 | g15028.t10 | Pfam | PF03062 | MBOAT, membrane-bound O-acyltransferase family | 166 | 485 | 3.1E-31 |
| 22 | g15028.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 60 | - |
| 34 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 61 | 81 | - |
| 14 | g15028.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 82 | 112 | - |
| 27 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 113 | 132 | - |
| 24 | g15028.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 133 | 143 | - |
| 32 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 144 | 163 | - |
| 18 | g15028.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 164 | 169 | - |
| 31 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 170 | 191 | - |
| 21 | g15028.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 192 | 261 | - |
| 29 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 262 | 280 | - |
| 17 | g15028.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 281 | 291 | - |
| 28 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 292 | 311 | - |
| 20 | g15028.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 312 | 341 | - |
| 33 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 342 | 360 | - |
| 16 | g15028.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 361 | 406 | - |
| 25 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 407 | 424 | - |
| 19 | g15028.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 425 | 435 | - |
| 26 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 436 | 453 | - |
| 15 | g15028.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 454 | 464 | - |
| 30 | g15028.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 465 | 486 | - |
| 23 | g15028.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 487 | 505 | - |
| 5 | g15028.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 64 | 81 | - |
| 7 | g15028.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 144 | 163 | - |
| 9 | g15028.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 168 | 190 | - |
| 8 | g15028.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 292 | 311 | - |
| 4 | g15028.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 341 | 363 | - |
| 10 | g15028.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 407 | 429 | - |
| 6 | g15028.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 465 | 487 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004144 | diacylglycerol O-acyltransferase activity | MF |
| GO:0019432 | triglyceride biosynthetic process | BP |
| GO:0008374 | O-acyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K11155 (diacylglycerol O-acyltransferase 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed