Gene loci information

Transcript annotation

  • This transcript has been annotated as Diacylglycerol O-acyltransferase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15028 g15028.t11 TTS g15028.t11 3587251 3587251
chr_4 g15028 g15028.t11 isoform g15028.t11 3587302 3590717
chr_4 g15028 g15028.t11 exon g15028.t11.exon1 3587302 3587487
chr_4 g15028 g15028.t11 cds g15028.t11.CDS1 3587302 3587487
chr_4 g15028 g15028.t11 exon g15028.t11.exon2 3587551 3587768
chr_4 g15028 g15028.t11 cds g15028.t11.CDS2 3587551 3587768
chr_4 g15028 g15028.t11 exon g15028.t11.exon3 3587838 3588375
chr_4 g15028 g15028.t11 cds g15028.t11.CDS3 3587838 3588375
chr_4 g15028 g15028.t11 exon g15028.t11.exon4 3588438 3588611
chr_4 g15028 g15028.t11 cds g15028.t11.CDS4 3588438 3588611
chr_4 g15028 g15028.t11 exon g15028.t11.exon5 3588700 3588752
chr_4 g15028 g15028.t11 cds g15028.t11.CDS5 3588700 3588752
chr_4 g15028 g15028.t11 exon g15028.t11.exon6 3588818 3589041
chr_4 g15028 g15028.t11 cds g15028.t11.CDS6 3588818 3589041
chr_4 g15028 g15028.t11 exon g15028.t11.exon7 3589409 3589543
chr_4 g15028 g15028.t11 cds g15028.t11.CDS7 3589409 3589536
chr_4 g15028 g15028.t11 exon g15028.t11.exon8 3590537 3590717
chr_4 g15028 g15028.t11 TSS g15028.t11 3590713 3590713

Sequences

>g15028.t11 Gene=g15028 Length=1709
AATTAGTTCTATGTGAAACTTCCGAGTGTAAAGTTGTTGATTATTTGGTTTCCTCGCGTG
TTCGTCTTTTATTTATTTCTAATCTATTTTATTTATTTAAATTTTGTAAAAGTTATTATT
TGGTTCAAAATAAATAGTCTTTGTTAAGTGTGCGTAATTGTGAAAAAAGTGAACAAGGTT
GAAATATTATGGCACCGTCTGAGAGCAATTGTAATGGTGAATTAAAAACGCGTCTGAGAC
GAACACAAAGCGTAACACGTGCTGAAGAGATCACTGATCAAGAGCAAAAGCAAAGAAAGA
GTCAACCAGATAAACCAGTACACAAGCCACGAGATTCTCTTTTTTCGTGGAGTTCAAATT
TCAATGATTTTAGTGGTTTGGTGAATTGGGGATTTCTTTTATTGACAATGGGAGGCATCA
GATTGCTACTAGAAAATTTTATAAAATATGGATTTCGAGTTGATCCAGTACAATGGCTAA
TTGTACTGAGTGGAAAAAATGAAGGAGATTCATCTTATCCATCGGTTGTTCTAGCTTTAT
ATTCAAGTGTGCCAATTGTTTTATGTATTATTATTGAAAAAGGACTTTCTGTGGAGATTA
TTCCAGAAACAACAGGCAAATTTGCACACGTGTTTAACTTGATTGCAGTAGTTCTTTTGC
CAATCGTTGTAATTAATTTACGTGGAACTTCATTTAGTTTGATGGGGTCAACATTTGTCT
GTTTTTTGTACTGTATACTTTTTGTAAAATTATGGAGTTATGTGCAGGTCAATTTATGGT
GTCGTCAAAATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGAAAGTTTTA
GTATAGCAGAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAATGAAAATT
TTATTGCTAATGATACAAAAGACCATGAACCACTGAAATTGATAGAGTATCCTGATAATT
TAAATCTTCGTGATCTTATCTATTTTCTTCTTGCGCCAACATTATGTTATGAATTAAATT
TCCCAAGAACAACTCGAATTCGTAAACGGTTTTTAATCAAAAGAATTTTAGAAGTAGTTG
TCGGAGTTAATATTGTCATGGCACTTTTTCAACAATGGATGATACCAAGTGTTAAAAATT
CTCTTATTCCTTTCTCAAATATGGAATTTACAAAAGCAACTGAACGTGTATTGAAGCTTG
CTATTCCAAATCATTTTATATGGCTGTGTGGGTTCTATCTAATTTTTCATTCAACCCTTA
ATCTTCTTGGTGAAATTTTACATTTTGCTGATCGCAATTTTTACTGTGACTGGTGGAACG
CAAATAACGTTGATACATTTTGGAAAACATGGAATATGCCTGTCCATCGTTGGTGTGTGA
GACATTTATATATTCCACTTGTTGATATGGGATATAGTCGTTTTGCTGCTTCTGGAGTTG
TTTTTTTCATTAGTGCCTTCTTCCACGAATATTTGGTATCAGTTCCTCTTCGAACATTTA
AAATTTGGGCTTTTATGGGAATGGTTTCGCAGTTACCTCTTTCTTATGTATCAAAATTTC
TCGAAAAAAATGTAGGAGAAAGATGGGGAAATATTTTAGTATGGGGAAGTTTAATTTTAG
GGCAACCACTGTGTATCATGGTCTATTATCATGACTATGTCATCCAAAATTATAATAAAG
TTATACTTGAACCTGAATATTTAGAATAG

>g15028.t11 Gene=g15028 Length=506
MAPSESNCNGELKTRLRRTQSVTRAEEITDQEQKQRKSQPDKPVHKPRDSLFSWSSNFND
FSGLVNWGFLLLTMGGIRLLLENFIKYGFRVDPVQWLIVLSGKNEGDSSYPSVVLALYSS
VPIVLCIIIEKGLSVEIIPETTGKFAHVFNLIAVVLLPIVVINLRGTSFSLMGSTFVCFL
YCILFVKLWSYVQVNLWCRQNQKNQKVSISGKRRESFSIAEFERKHHRNSNTDQNENFIA
NDTKDHEPLKLIEYPDNLNLRDLIYFLLAPTLCYELNFPRTTRIRKRFLIKRILEVVVGV
NIVMALFQQWMIPSVKNSLIPFSNMEFTKATERVLKLAIPNHFIWLCGFYLIFHSTLNLL
GEILHFADRNFYCDWWNANNVDTFWKTWNMPVHRWCVRHLYIPLVDMGYSRFAASGVVFF
ISAFFHEYLVSVPLRTFKIWAFMGMVSQLPLSYVSKFLEKNVGERWGNILVWGSLILGQP
LCIMVYYHDYVIQNYNKVILEPEYLE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
11 g15028.t11 MobiDBLite mobidb-lite consensus disorder prediction 1 16 -
12 g15028.t11 MobiDBLite mobidb-lite consensus disorder prediction 1 46 -
13 g15028.t11 MobiDBLite mobidb-lite consensus disorder prediction 17 46 -
2 g15028.t11 PANTHER PTHR10408:SF7 DIACYLGLYCEROL O-ACYLTRANSFERASE 1 17 494 8.5E-154
3 g15028.t11 PANTHER PTHR10408 STEROL O-ACYLTRANSFERASE 17 494 8.5E-154
35 g15028.t11 PIRSF PIRSF000439 Oat_ACAT_DAG_ARE 2 497 7.7E-140
36 g15028.t11 PIRSF PIRSF500231 Oat_dag 2 498 2.1E-175
1 g15028.t11 Pfam PF03062 MBOAT, membrane-bound O-acyltransferase family 167 486 3.1E-31
22 g15028.t11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 60 -
33 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 61 81 -
15 g15028.t11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 82 112 -
30 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 113 133 -
24 g15028.t11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 134 144 -
31 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 145 164 -
18 g15028.t11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 165 170 -
29 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 171 192 -
20 g15028.t11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 193 262 -
27 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 263 281 -
17 g15028.t11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 282 292 -
34 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 293 312 -
19 g15028.t11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 313 342 -
25 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 343 361 -
14 g15028.t11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 362 407 -
26 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 408 425 -
21 g15028.t11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 426 436 -
32 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 437 454 -
16 g15028.t11 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 455 465 -
28 g15028.t11 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 466 487 -
23 g15028.t11 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 488 506 -
4 g15028.t11 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 64 81 -
8 g15028.t11 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 145 164 -
5 g15028.t11 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 169 191 -
10 g15028.t11 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 293 312 -
9 g15028.t11 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 342 364 -
7 g15028.t11 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 408 430 -
6 g15028.t11 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 466 488 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004144 diacylglycerol O-acyltransferase activity MF
GO:0019432 triglyceride biosynthetic process BP
GO:0008374 O-acyltransferase activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed