General information for g15028.t12
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Diacylglycerol O-acyltransferase 1.
Parent gene
- The parent gene g15028 is located on chr_4: 3588891..3590717 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15028 | g15028.t12 | TTS | g15028.t12 | 3587251 | 3587251 |
| chr_4 | g15028 | g15028.t12 | isoform | g15028.t12 | 3587302 | 3590717 |
| chr_4 | g15028 | g15028.t12 | exon | g15028.t12.exon1 | 3587302 | 3587487 |
| chr_4 | g15028 | g15028.t12 | cds | g15028.t12.CDS1 | 3587302 | 3587487 |
| chr_4 | g15028 | g15028.t12 | exon | g15028.t12.exon2 | 3587551 | 3587768 |
| chr_4 | g15028 | g15028.t12 | cds | g15028.t12.CDS2 | 3587551 | 3587768 |
| chr_4 | g15028 | g15028.t12 | exon | g15028.t12.exon3 | 3587838 | 3588375 |
| chr_4 | g15028 | g15028.t12 | cds | g15028.t12.CDS3 | 3587838 | 3588375 |
| chr_4 | g15028 | g15028.t12 | exon | g15028.t12.exon4 | 3588438 | 3588611 |
| chr_4 | g15028 | g15028.t12 | cds | g15028.t12.CDS4 | 3588438 | 3588503 |
| chr_4 | g15028 | g15028.t12 | exon | g15028.t12.exon5 | 3588696 | 3588752 |
| chr_4 | g15028 | g15028.t12 | exon | g15028.t12.exon6 | 3588818 | 3589041 |
| chr_4 | g15028 | g15028.t12 | exon | g15028.t12.exon7 | 3589409 | 3589551 |
| chr_4 | g15028 | g15028.t12 | exon | g15028.t12.exon8 | 3590541 | 3590717 |
| chr_4 | g15028 | g15028.t12 | TSS | g15028.t12 | 3590713 | 3590713 |
Sequences
>g15028.t12 Gene=g15028 Length=1717
AATTAGTTCTATGTGAAACTTCCGAGTGTAAAGTTGTTGATTATTTGGTTTCCTCGCGTG
TTCGTCTTTTATTTATTTCTAATCTATTTTATTTATTTAAATTTTGTAAAAGTTATTATT
TGGTTCAAAATAAATAGTCTTTGTTAAGTGTGCGTAATTGTGAAAAAAGTGAACAAGTTT
AATAGAAATATTATGGCACCGTCTGAGAGCAATTGTAATGGTGAATTAAAAACGCGTCTG
AGACGAACACAAAGCGTAACACGTGCTGAAGAGATCACTGATCAAGAGCAAAAGCAAAGA
AAGAGTCAACCAGATAAACCAGTACACAAGCCACGAGATTCTCTTTTTTCGTGGAGTTCA
AATTTCAATGATTTTAGTGGTTTGGTGAATTGGGGATTTCTTTTATTGACAATGGGAGGC
ATCAGATTGCTACTAGAAAATTTTATAAAATATGGATTTCGAGTTGATCCAGTACAATGG
CTAATTGTACTGAGTGGAAAAAATGAAGGAGATTCATCTTATCCATCGGTTGTTCTAGCT
TTATATTCAAGTGTGCCAATTGTTTTATGTATTATTATTGAAAAAGGACTTTCTGTGGTA
AGAGATTATTCCAGAAACAACAGGCAAATTTGCACACGTGTTTAACTTGATTGCAGTAGT
TCTTTTGCCAATCGTTGTAATTAATTTACGTGGAACTTCATTTAGTTTGATGGGGTCAAC
ATTTGTCTGTTTTTTGTACTGTATACTTTTTGTAAAATTATGGAGTTATGTGCAGGTCAA
TTTATGGTGTCGTCAAAATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGA
AAGTTTTAGTATAGCAGAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAA
TGAAAATTTTATTGCTAATGATACAAAAGACCATGAACCACTGAAATTGATAGAGTATCC
TGATAATTTAAATCTTCGTGATCTTATCTATTTTCTTCTTGCGCCAACATTATGTTATGA
ATTAAATTTCCCAAGAACAACTCGAATTCGTAAACGGTTTTTAATCAAAAGAATTTTAGA
AGTAGTTGTCGGAGTTAATATTGTCATGGCACTTTTTCAACAATGGATGATACCAAGTGT
TAAAAATTCTCTTATTCCTTTCTCAAATATGGAATTTACAAAAGCAACTGAACGTGTATT
GAAGCTTGCTATTCCAAATCATTTTATATGGCTGTGTGGGTTCTATCTAATTTTTCATTC
AACCCTTAATCTTCTTGGTGAAATTTTACATTTTGCTGATCGCAATTTTTACTGTGACTG
GTGGAACGCAAATAACGTTGATACATTTTGGAAAACATGGAATATGCCTGTCCATCGTTG
GTGTGTGAGACATTTATATATTCCACTTGTTGATATGGGATATAGTCGTTTTGCTGCTTC
TGGAGTTGTTTTTTTCATTAGTGCCTTCTTCCACGAATATTTGGTATCAGTTCCTCTTCG
AACATTTAAAATTTGGGCTTTTATGGGAATGGTTTCGCAGTTACCTCTTTCTTATGTATC
AAAATTTCTCGAAAAAAATGTAGGAGAAAGATGGGGAAATATTTTAGTATGGGGAAGTTT
AATTTTAGGGCAACCACTGTGTATCATGGTCTATTATCATGACTATGTCATCCAAAATTA
TAATAAAGTTATACTTGAACCTGAATATTTAGAATAG
>g15028.t12 Gene=g15028 Length=335
MGSTFVCFLYCILFVKLWSYVQVNLWCRQNQKNQKVSISGKRRESFSIAEFERKHHRNSN
TDQNENFIANDTKDHEPLKLIEYPDNLNLRDLIYFLLAPTLCYELNFPRTTRIRKRFLIK
RILEVVVGVNIVMALFQQWMIPSVKNSLIPFSNMEFTKATERVLKLAIPNHFIWLCGFYL
IFHSTLNLLGEILHFADRNFYCDWWNANNVDTFWKTWNMPVHRWCVRHLYIPLVDMGYSR
FAASGVVFFISAFFHEYLVSVPLRTFKIWAFMGMVSQLPLSYVSKFLEKNVGERWGNILV
WGSLILGQPLCIMVYYHDYVIQNYNKVILEPEYLE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g15028.t12 | PANTHER | PTHR10408:SF7 | DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 2 | 323 | 6.8E-122 |
| 3 | g15028.t12 | PANTHER | PTHR10408 | STEROL O-ACYLTRANSFERASE | 2 | 323 | 6.8E-122 |
| 27 | g15028.t12 | PIRSF | PIRSF000439 | Oat_ACAT_DAG_ARE | 1 | 326 | 3.6E-116 |
| 1 | g15028.t12 | Pfam | PF03062 | MBOAT, membrane-bound O-acyltransferase family | 5 | 315 | 2.5E-31 |
| 17 | g15028.t12 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 18 | g15028.t12 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 19 | g15028.t12 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 26 | g15028.t12 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 19 | - |
| 13 | g15028.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 91 | - |
| 24 | g15028.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 92 | 110 | - |
| 12 | g15028.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 111 | 121 | - |
| 22 | g15028.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 141 | - |
| 16 | g15028.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 142 | 171 | - |
| 21 | g15028.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 172 | 190 | - |
| 10 | g15028.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 191 | 236 | - |
| 25 | g15028.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 237 | 254 | - |
| 15 | g15028.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 255 | 265 | - |
| 23 | g15028.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 266 | 283 | - |
| 11 | g15028.t12 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 284 | 294 | - |
| 20 | g15028.t12 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 295 | 316 | - |
| 14 | g15028.t12 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 317 | 335 | - |
| 9 | g15028.t12 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 5 | g15028.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 4 | 26 | - |
| 6 | g15028.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 122 | 141 | - |
| 4 | g15028.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 171 | 193 | - |
| 8 | g15028.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 237 | 259 | - |
| 7 | g15028.t12 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 295 | 317 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004144 | diacylglycerol O-acyltransferase activity | MF |
| GO:0019432 | triglyceride biosynthetic process | BP |
| GO:0008374 | O-acyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K11155 (diacylglycerol O-acyltransferase 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
