Gene loci information

Transcript annotation

  • This transcript has been annotated as Diacylglycerol O-acyltransferase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15028 g15028.t13 TTS g15028.t13 3587251 3587251
chr_4 g15028 g15028.t13 isoform g15028.t13 3587302 3590717
chr_4 g15028 g15028.t13 exon g15028.t13.exon1 3587302 3587487
chr_4 g15028 g15028.t13 cds g15028.t13.CDS1 3587302 3587487
chr_4 g15028 g15028.t13 exon g15028.t13.exon2 3587551 3587768
chr_4 g15028 g15028.t13 cds g15028.t13.CDS2 3587551 3587768
chr_4 g15028 g15028.t13 exon g15028.t13.exon3 3587838 3588375
chr_4 g15028 g15028.t13 cds g15028.t13.CDS3 3587838 3588375
chr_4 g15028 g15028.t13 exon g15028.t13.exon4 3588438 3588598
chr_4 g15028 g15028.t13 cds g15028.t13.CDS4 3588438 3588598
chr_4 g15028 g15028.t13 exon g15028.t13.exon5 3588696 3588752
chr_4 g15028 g15028.t13 cds g15028.t13.CDS5 3588696 3588752
chr_4 g15028 g15028.t13 exon g15028.t13.exon6 3588818 3589041
chr_4 g15028 g15028.t13 cds g15028.t13.CDS6 3588818 3589041
chr_4 g15028 g15028.t13 exon g15028.t13.exon7 3589409 3589551
chr_4 g15028 g15028.t13 cds g15028.t13.CDS7 3589409 3589536
chr_4 g15028 g15028.t13 exon g15028.t13.exon8 3590541 3590717
chr_4 g15028 g15028.t13 TSS g15028.t13 3590713 3590713

Sequences

>g15028.t13 Gene=g15028 Length=1704
AATTAGTTCTATGTGAAACTTCCGAGTGTAAAGTTGTTGATTATTTGGTTTCCTCGCGTG
TTCGTCTTTTATTTATTTCTAATCTATTTTATTTATTTAAATTTTGTAAAAGTTATTATT
TGGTTCAAAATAAATAGTCTTTGTTAAGTGTGCGTAATTGTGAAAAAAGTGAACAAGTTT
AATAGAAATATTATGGCACCGTCTGAGAGCAATTGTAATGGTGAATTAAAAACGCGTCTG
AGACGAACACAAAGCGTAACACGTGCTGAAGAGATCACTGATCAAGAGCAAAAGCAAAGA
AAGAGTCAACCAGATAAACCAGTACACAAGCCACGAGATTCTCTTTTTTCGTGGAGTTCA
AATTTCAATGATTTTAGTGGTTTGGTGAATTGGGGATTTCTTTTATTGACAATGGGAGGC
ATCAGATTGCTACTAGAAAATTTTATAAAATATGGATTTCGAGTTGATCCAGTACAATGG
CTAATTGTACTGAGTGGAAAAAATGAAGGAGATTCATCTTATCCATCGGTTGTTCTAGCT
TTATATTCAAGTGTGCCAATTGTTTTATGTATTATTATTGAAAAAGGACTTTCTGTGGTA
AAAACAACAGGCAAATTTGCACACGTGTTTAACTTGATTGCAGTAGTTCTTTTGCCAATC
GTTGTAATTAATTTACGTGGAACTTCATTTAGTTTGATGGGGTCAACATTTGTCTGTTTT
TTGTACTGTATACTTTTTGTAAAATTATGGAGTTATGTGCAGGTCAATTTATGGTGTCGT
CAAAATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGAAAGTTTTAGTATA
GCAGAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAATGAAAATTTTATT
GCTAATGATACAAAAGACCATGAACCACTGAAATTGATAGAGTATCCTGATAATTTAAAT
CTTCGTGATCTTATCTATTTTCTTCTTGCGCCAACATTATGTTATGAATTAAATTTCCCA
AGAACAACTCGAATTCGTAAACGGTTTTTAATCAAAAGAATTTTAGAAGTAGTTGTCGGA
GTTAATATTGTCATGGCACTTTTTCAACAATGGATGATACCAAGTGTTAAAAATTCTCTT
ATTCCTTTCTCAAATATGGAATTTACAAAAGCAACTGAACGTGTATTGAAGCTTGCTATT
CCAAATCATTTTATATGGCTGTGTGGGTTCTATCTAATTTTTCATTCAACCCTTAATCTT
CTTGGTGAAATTTTACATTTTGCTGATCGCAATTTTTACTGTGACTGGTGGAACGCAAAT
AACGTTGATACATTTTGGAAAACATGGAATATGCCTGTCCATCGTTGGTGTGTGAGACAT
TTATATATTCCACTTGTTGATATGGGATATAGTCGTTTTGCTGCTTCTGGAGTTGTTTTT
TTCATTAGTGCCTTCTTCCACGAATATTTGGTATCAGTTCCTCTTCGAACATTTAAAATT
TGGGCTTTTATGGGAATGGTTTCGCAGTTACCTCTTTCTTATGTATCAAAATTTCTCGAA
AAAAATGTAGGAGAAAGATGGGGAAATATTTTAGTATGGGGAAGTTTAATTTTAGGGCAA
CCACTGTGTATCATGGTCTATTATCATGACTATGTCATCCAAAATTATAATAAAGTTATA
CTTGAACCTGAATATTTAGAATAG

>g15028.t13 Gene=g15028 Length=503
MAPSESNCNGELKTRLRRTQSVTRAEEITDQEQKQRKSQPDKPVHKPRDSLFSWSSNFND
FSGLVNWGFLLLTMGGIRLLLENFIKYGFRVDPVQWLIVLSGKNEGDSSYPSVVLALYSS
VPIVLCIIIEKGLSVVKTTGKFAHVFNLIAVVLLPIVVINLRGTSFSLMGSTFVCFLYCI
LFVKLWSYVQVNLWCRQNQKNQKVSISGKRRESFSIAEFERKHHRNSNTDQNENFIANDT
KDHEPLKLIEYPDNLNLRDLIYFLLAPTLCYELNFPRTTRIRKRFLIKRILEVVVGVNIV
MALFQQWMIPSVKNSLIPFSNMEFTKATERVLKLAIPNHFIWLCGFYLIFHSTLNLLGEI
LHFADRNFYCDWWNANNVDTFWKTWNMPVHRWCVRHLYIPLVDMGYSRFAASGVVFFISA
FFHEYLVSVPLRTFKIWAFMGMVSQLPLSYVSKFLEKNVGERWGNILVWGSLILGQPLCI
MVYYHDYVIQNYNKVILEPEYLE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
12 g15028.t13 MobiDBLite mobidb-lite consensus disorder prediction 1 16 -
13 g15028.t13 MobiDBLite mobidb-lite consensus disorder prediction 1 46 -
14 g15028.t13 MobiDBLite mobidb-lite consensus disorder prediction 17 46 -
2 g15028.t13 PANTHER PTHR10408:SF7 DIACYLGLYCEROL O-ACYLTRANSFERASE 1 17 491 1.2E-150
3 g15028.t13 PANTHER PTHR10408 STEROL O-ACYLTRANSFERASE 17 491 1.2E-150
34 g15028.t13 PIRSF PIRSF000439 Oat_ACAT_DAG_ARE 2 494 1.3E-137
35 g15028.t13 PIRSF PIRSF500231 Oat_dag 2 495 2.0E-171
1 g15028.t13 Pfam PF03062 MBOAT, membrane-bound O-acyltransferase family 164 483 3.1E-31
18 g15028.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 63 -
27 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 64 81 -
24 g15028.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 82 109 -
32 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 110 130 -
19 g15028.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 131 141 -
31 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 142 161 -
23 g15028.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 162 166 -
26 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 167 189 -
16 g15028.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 190 289 -
29 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 290 309 -
22 g15028.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 310 339 -
25 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 340 358 -
17 g15028.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 359 404 -
33 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 405 422 -
20 g15028.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 423 433 -
30 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 434 451 -
15 g15028.t13 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 452 462 -
28 g15028.t13 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 463 484 -
21 g15028.t13 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 485 503 -
5 g15028.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 64 81 -
4 g15028.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 110 129 -
8 g15028.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 142 161 -
10 g15028.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 166 188 -
6 g15028.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 290 309 -
11 g15028.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 339 361 -
9 g15028.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 405 427 -
7 g15028.t13 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 463 485 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004144 diacylglycerol O-acyltransferase activity MF
GO:0019432 triglyceride biosynthetic process BP
GO:0008374 O-acyltransferase activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed