General information for g15028.t16
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Diacylglycerol O-acyltransferase 1.
Parent gene
- The parent gene g15028 is located on chr_4: 3588891..3590717 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15028 | g15028.t16 | TTS | g15028.t16 | 3587251 | 3587251 |
| chr_4 | g15028 | g15028.t16 | isoform | g15028.t16 | 3587836 | 3590717 |
| chr_4 | g15028 | g15028.t16 | exon | g15028.t16.exon1 | 3587836 | 3588375 |
| chr_4 | g15028 | g15028.t16 | cds | g15028.t16.CDS1 | 3587836 | 3588375 |
| chr_4 | g15028 | g15028.t16 | exon | g15028.t16.exon2 | 3588438 | 3588611 |
| chr_4 | g15028 | g15028.t16 | cds | g15028.t16.CDS2 | 3588438 | 3588611 |
| chr_4 | g15028 | g15028.t16 | exon | g15028.t16.exon3 | 3588700 | 3588752 |
| chr_4 | g15028 | g15028.t16 | cds | g15028.t16.CDS3 | 3588700 | 3588752 |
| chr_4 | g15028 | g15028.t16 | exon | g15028.t16.exon4 | 3588818 | 3589041 |
| chr_4 | g15028 | g15028.t16 | cds | g15028.t16.CDS4 | 3588818 | 3589041 |
| chr_4 | g15028 | g15028.t16 | exon | g15028.t16.exon5 | 3589409 | 3589551 |
| chr_4 | g15028 | g15028.t16 | cds | g15028.t16.CDS5 | 3589409 | 3589536 |
| chr_4 | g15028 | g15028.t16 | exon | g15028.t16.exon6 | 3590541 | 3590717 |
| chr_4 | g15028 | g15028.t16 | TSS | g15028.t16 | 3590713 | 3590713 |
Sequences
>g15028.t16 Gene=g15028 Length=1311
AATTAGTTCTATGTGAAACTTCCGAGTGTAAAGTTGTTGATTATTTGGTTTCCTCGCGTG
TTCGTCTTTTATTTATTTCTAATCTATTTTATTTATTTAAATTTTGTAAAAGTTATTATT
TGGTTCAAAATAAATAGTCTTTGTTAAGTGTGCGTAATTGTGAAAAAAGTGAACAAGTTT
AATAGAAATATTATGGCACCGTCTGAGAGCAATTGTAATGGTGAATTAAAAACGCGTCTG
AGACGAACACAAAGCGTAACACGTGCTGAAGAGATCACTGATCAAGAGCAAAAGCAAAGA
AAGAGTCAACCAGATAAACCAGTACACAAGCCACGAGATTCTCTTTTTTCGTGGAGTTCA
AATTTCAATGATTTTAGTGGTTTGGTGAATTGGGGATTTCTTTTATTGACAATGGGAGGC
ATCAGATTGCTACTAGAAAATTTTATAAAATATGGATTTCGAGTTGATCCAGTACAATGG
CTAATTGTACTGAGTGGAAAAAATGAAGGAGATTCATCTTATCCATCGGTTGTTCTAGCT
TTATATTCAAGTGTGCCAATTGTTTTATGTATTATTATTGAAAAAGGACTTTCTGTGGAG
ATTATTCCAGAAACAACAGGCAAATTTGCACACGTGTTTAACTTGATTGCAGTAGTTCTT
TTGCCAATCGTTGTAATTAATTTACGTGGAACTTCATTTAGTTTGATGGGGTCAACATTT
GTCTGTTTTTTGTACTGTATACTTTTTGTAAAATTATGGAGTTATGTGCAGGTCAATTTA
TGGTGTCGTCAAAATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGAAAGT
TTTAGTATAGCAGAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAATGAA
AATTTTATTGCTAATGATACAAAAGACCATGAACCACTGAAATTGATAGAGTATCCTGAT
AATTTAAATCTTCGTGATCTTATCTATTTTCTTCTTGCGCCAACATTATGTTATGAATTA
AATTTCCCAAGAACAACTCGAATTCGTAAACGGTTTTTAATCAAAAGAATTTTAGAAGTA
GTTGTCGGAGTTAATATTGTCATGGCACTTTTTCAACAATGGATGATACCAAGTGTTAAA
AATTCTCTTATTCCTTTCTCAAATATGGAATTTACAAAAGCAACTGAACGTGTATTGAAG
CTTGCTATTCCAAATCATTTTATATGGCTGTGTGGGTTCTATCTAATTTTTCATTCAACC
CTTAATCTTCTTGGTGAAATTTTACATTTTGCTGATCGCAATTTTTACTGT
>g15028.t16 Gene=g15028 Length=373
MAPSESNCNGELKTRLRRTQSVTRAEEITDQEQKQRKSQPDKPVHKPRDSLFSWSSNFND
FSGLVNWGFLLLTMGGIRLLLENFIKYGFRVDPVQWLIVLSGKNEGDSSYPSVVLALYSS
VPIVLCIIIEKGLSVEIIPETTGKFAHVFNLIAVVLLPIVVINLRGTSFSLMGSTFVCFL
YCILFVKLWSYVQVNLWCRQNQKNQKVSISGKRRESFSIAEFERKHHRNSNTDQNENFIA
NDTKDHEPLKLIEYPDNLNLRDLIYFLLAPTLCYELNFPRTTRIRKRFLIKRILEVVVGV
NIVMALFQQWMIPSVKNSLIPFSNMEFTKATERVLKLAIPNHFIWLCGFYLIFHSTLNLL
GEILHFADRNFYC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g15028.t16 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 16 | - |
| 10 | g15028.t16 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 46 | - |
| 11 | g15028.t16 | MobiDBLite | mobidb-lite | consensus disorder prediction | 17 | 46 | - |
| 2 | g15028.t16 | PANTHER | PTHR10408:SF7 | DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 17 | 372 | 1.2E-90 |
| 3 | g15028.t16 | PANTHER | PTHR10408 | STEROL O-ACYLTRANSFERASE | 17 | 372 | 1.2E-90 |
| 27 | g15028.t16 | PIRSF | PIRSF000439 | Oat_ACAT_DAG_ARE | 2 | 373 | 3.1E-86 |
| 1 | g15028.t16 | Pfam | PF03062 | MBOAT, membrane-bound O-acyltransferase family | 167 | 363 | 7.8E-8 |
| 18 | g15028.t16 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 60 | - |
| 26 | g15028.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 61 | 81 | - |
| 12 | g15028.t16 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 82 | 112 | - |
| 23 | g15028.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 113 | 133 | - |
| 19 | g15028.t16 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 134 | 144 | - |
| 22 | g15028.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 145 | 164 | - |
| 15 | g15028.t16 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 165 | 170 | - |
| 21 | g15028.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 171 | 192 | - |
| 17 | g15028.t16 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 193 | 262 | - |
| 24 | g15028.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 263 | 281 | - |
| 14 | g15028.t16 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 282 | 292 | - |
| 25 | g15028.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 293 | 312 | - |
| 16 | g15028.t16 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 313 | 342 | - |
| 20 | g15028.t16 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 343 | 361 | - |
| 13 | g15028.t16 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 362 | 373 | - |
| 4 | g15028.t16 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 64 | 81 | - |
| 6 | g15028.t16 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 145 | 164 | - |
| 5 | g15028.t16 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 169 | 191 | - |
| 8 | g15028.t16 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 293 | 312 | - |
| 7 | g15028.t16 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 342 | 364 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004144 | diacylglycerol O-acyltransferase activity | MF |
| GO:0019432 | triglyceride biosynthetic process | BP |
| GO:0008374 | O-acyltransferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
