Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Diacylglycerol O-acyltransferase 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15028 g15028.t20 TTS g15028.t20 3587251 3587251
chr_4 g15028 g15028.t20 isoform g15028.t20 3588164 3590717
chr_4 g15028 g15028.t20 exon g15028.t20.exon1 3588164 3588375
chr_4 g15028 g15028.t20 cds g15028.t20.CDS1 3588166 3588375
chr_4 g15028 g15028.t20 exon g15028.t20.exon2 3588438 3588611
chr_4 g15028 g15028.t20 cds g15028.t20.CDS2 3588438 3588611
chr_4 g15028 g15028.t20 exon g15028.t20.exon3 3588703 3588752
chr_4 g15028 g15028.t20 cds g15028.t20.CDS3 3588703 3588752
chr_4 g15028 g15028.t20 exon g15028.t20.exon4 3588818 3589041
chr_4 g15028 g15028.t20 cds g15028.t20.CDS4 3588818 3589041
chr_4 g15028 g15028.t20 exon g15028.t20.exon5 3589409 3589551
chr_4 g15028 g15028.t20 cds g15028.t20.CDS5 3589409 3589536
chr_4 g15028 g15028.t20 exon g15028.t20.exon6 3590541 3590717
chr_4 g15028 g15028.t20 TSS g15028.t20 3590713 3590713

Sequences

>g15028.t20 Gene=g15028 Length=980
AATTAGTTCTATGTGAAACTTCCGAGTGTAAAGTTGTTGATTATTTGGTTTCCTCGCGTG
TTCGTCTTTTATTTATTTCTAATCTATTTTATTTATTTAAATTTTGTAAAAGTTATTATT
TGGTTCAAAATAAATAGTCTTTGTTAAGTGTGCGTAATTGTGAAAAAAGTGAACAAGTTT
AATAGAAATATTATGGCACCGTCTGAGAGCAATTGTAATGGTGAATTAAAAACGCGTCTG
AGACGAACACAAAGCGTAACACGTGCTGAAGAGATCACTGATCAAGAGCAAAAGCAAAGA
AAGAGTCAACCAGATAAACCAGTACACAAGCCACGAGATTCTCTTTTTTCGTGGAGTTCA
AATTTCAATGATTTTAGTGGTTTGGTGAATTGGGGATTTCTTTTATTGACAATGGGAGGC
ATCAGATTGCTACTAGAAAATTTTATAAAATATGGATTTCGAGTTGATCCAGTACAATGG
CTAATTGTACTGAGTGGAAAAAATGAAGGAGATTCATCTTATCCATCGGTTGTTCTAGCT
TTATATTCAAGTGTGCCAATTGTTTTATGTATTATTATTGAAAAAGGACTTTCTGAGATT
ATTCCAGAAACAACAGGCAAATTTGCACACGTGTTTAACTTGATTGCAGTAGTTCTTTTG
CCAATCGTTGTAATTAATTTACGTGGAACTTCATTTAGTTTGATGGGGTCAACATTTGTC
TGTTTTTTGTACTGTATACTTTTTGTAAAATTATGGAGTTATGTGCAGGTCAATTTATGG
TGTCGTCAAAATCAAAAGAATCAAAAAGTGTCAATTTCGGGAAAGAGACGAGAAAGTTTT
AGTATAGCAGAATTTGAAAGAAAACATCATAGAAACAGCAATACAGATCAAAATGAAAAT
TTTATTGCTAATGATACAAAAGACCATGAACCACTGAAATTGATAGAGTATCCTGATAAT
TTAAATCTTCGTGATCTTAT

>g15028.t20 Gene=g15028 Length=262
MAPSESNCNGELKTRLRRTQSVTRAEEITDQEQKQRKSQPDKPVHKPRDSLFSWSSNFND
FSGLVNWGFLLLTMGGIRLLLENFIKYGFRVDPVQWLIVLSGKNEGDSSYPSVVLALYSS
VPIVLCIIIEKGLSEIIPETTGKFAHVFNLIAVVLLPIVVINLRGTSFSLMGSTFVCFLY
CILFVKLWSYVQVNLWCRQNQKNQKVSISGKRRESFSIAEFERKHHRNSNTDQNENFIAN
DTKDHEPLKLIEYPDNLNLRDL

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g15028.t20 Coils Coil Coil 259 262 -
6 g15028.t20 MobiDBLite mobidb-lite consensus disorder prediction 1 16 -
7 g15028.t20 MobiDBLite mobidb-lite consensus disorder prediction 1 46 -
8 g15028.t20 MobiDBLite mobidb-lite consensus disorder prediction 17 46 -
1 g15028.t20 PANTHER PTHR10408:SF7 DIACYLGLYCEROL O-ACYLTRANSFERASE 1 17 261 5.4E-33
2 g15028.t20 PANTHER PTHR10408 STEROL O-ACYLTRANSFERASE 17 261 5.4E-33
13 g15028.t20 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 60 -
18 g15028.t20 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 61 81 -
10 g15028.t20 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 82 112 -
17 g15028.t20 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 113 132 -
14 g15028.t20 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 133 143 -
16 g15028.t20 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 144 163 -
11 g15028.t20 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 164 169 -
15 g15028.t20 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 170 191 -
12 g15028.t20 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 192 262 -
3 g15028.t20 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 64 81 -
4 g15028.t20 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 144 163 -
5 g15028.t20 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 168 190 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0004144 diacylglycerol O-acyltransferase activity MF
GO:0019432 triglyceride biosynthetic process BP
GO:0008374 O-acyltransferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values