Gene loci information

Transcript annotation

  • This transcript has been annotated as Cytochrome P450 4d1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g15085 g15085.t15 isoform g15085.t15 3852337 3854630
chr_4 g15085 g15085.t15 exon g15085.t15.exon1 3852337 3852877
chr_4 g15085 g15085.t15 cds g15085.t15.CDS1 3852337 3852877
chr_4 g15085 g15085.t15 exon g15085.t15.exon2 3852942 3853011
chr_4 g15085 g15085.t15 cds g15085.t15.CDS2 3852942 3853011
chr_4 g15085 g15085.t15 exon g15085.t15.exon3 3853080 3853436
chr_4 g15085 g15085.t15 cds g15085.t15.CDS3 3853080 3853436
chr_4 g15085 g15085.t15 exon g15085.t15.exon4 3853957 3854177
chr_4 g15085 g15085.t15 cds g15085.t15.CDS4 3853957 3854177
chr_4 g15085 g15085.t15 exon g15085.t15.exon5 3854240 3854402
chr_4 g15085 g15085.t15 cds g15085.t15.CDS5 3854240 3854402
chr_4 g15085 g15085.t15 exon g15085.t15.exon6 3854462 3854630
chr_4 g15085 g15085.t15 cds g15085.t15.CDS6 3854462 3854630
chr_4 g15085 g15085.t15 TTS g15085.t15 3855622 3855622
chr_4 g15085 g15085.t15 TSS g15085.t15 NA NA

Sequences

>g15085.t15 Gene=g15085 Length=1521
ATGGACACTCTTGTATTTGTGATAATTTCAATTTGCATATTTTTCGGTTGGTACTATAAC
AAGTATCGCGAAAGAATGGCAATAGTTGCAAAATTACCTGGACCATATCGATGGCCATTT
GTTGGAAATGCAGCACTGTTCATTTGGAAATCACCACCTGAAATGATTAATATAATAACA
GAAATTCATCGGCAATTTCCTCGTATCACTTGCCTCATGCTTGGTCCGCAACCAGAAATT
TTAATCAGTGATCCCAAATTAGCTGAGGTTGTTTTGGGTAGCCAAAAATTGATTGATAAA
TCTGATGAGTATTCATTCATAAGTGATTGGCTAGCAACTGGACTTTTGACAGCAACTGGT
AAAAAATGGTTCCAACGACGCAAAGTCATCACACCGGCATTTCATTTTAAAATTCTTGAC
CAATTTGTTGAAATTTTTGATAAGCAAAGTGCAGTTTTCGTGGAGAATCTTGCAAAATCA
AAAGGTCAACCTTTGGACGTTTTTCAACCTATCACACTTTGCGCACTCGACATTATCTGT
GAAACTGCAATGGGTGTACAAATACAAGCACAAATTAATTCAGATTCTGTTTATGTACAA
GCTGTAAAAGAAATCAGTGGACTATTAAGTAAGAGGATTTTCACTTTTTATCTTCGAAAT
GATTTTATATTCAATTTAACACCATTGAAAAGACGACAAGATAAATTGTTGAAAATTTTA
CATGGTTTTACTGATGAAGTGATTGTTTCACGACGTGCTGAACTTATGAATGATAAAAAA
CAAGATTCTTCATCAAATCATGATCAAGATGATATTGGAGTTAAAAAGAAGATGGCATTT
CTTGATATTTTGTTGCAGTCAACAATTGAAGGGAAACCATTGACAAATATGGATATAAGA
GAAGAAGTGGATACTTTTATGTTTGAGGGCCATGACACAACTAGCAGGTTCGCAGTGTGT
TTAATTGGTGGAATCGCCTTTTGCCTTTACAATCTTGCTAAATATCCTGACGTACAAAAG
AAAGCATATGAAGAAGTTCGACATGTGATCGGTGATGATTTGGATACTCCAGTTACAATA
AAACTTCTCAATGATCTCAATTATCTTGAAATGGTTATAAAAGAGACTTTGAGACTTTAT
CCAAGTGTTCCTATGTATGGTAGAAAAATGCAAGAAAATATTGACATGAATGGATTTATA
ATTCCAAAAGGTTCAAATATTGGTATTTCACCTTATTTTATGGCTCGTGATCCAACATTG
TGGGAAAATCCTTTTGAATTTATTCCTGAAAGATTCGAGTCTGATAATCAATATCATCCT
TATTTAAATGTTCCATTCAGTGCTGGTCCAAGAAATTGCATCGGACAAAAGTTTGCAATG
CTTGAAATGAAGAGCACAATAAGTAAAGTATTGAGAAACTTTGAACTATCAGTTGCACCC
GGTTATGAGCCAATTTTAGTTGCTGAATTAATTTTAAGACCTGATAATGGTGTCATGCTT
ATTTTGAATAAACGTAAATAA

>g15085.t15 Gene=g15085 Length=506
MDTLVFVIISICIFFGWYYNKYRERMAIVAKLPGPYRWPFVGNAALFIWKSPPEMINIIT
EIHRQFPRITCLMLGPQPEILISDPKLAEVVLGSQKLIDKSDEYSFISDWLATGLLTATG
KKWFQRRKVITPAFHFKILDQFVEIFDKQSAVFVENLAKSKGQPLDVFQPITLCALDIIC
ETAMGVQIQAQINSDSVYVQAVKEISGLLSKRIFTFYLRNDFIFNLTPLKRRQDKLLKIL
HGFTDEVIVSRRAELMNDKKQDSSSNHDQDDIGVKKKMAFLDILLQSTIEGKPLTNMDIR
EEVDTFMFEGHDTTSRFAVCLIGGIAFCLYNLAKYPDVQKKAYEEVRHVIGDDLDTPVTI
KLLNDLNYLEMVIKETLRLYPSVPMYGRKMQENIDMNGFIIPKGSNIGISPYFMARDPTL
WENPFEFIPERFESDNQYHPYLNVPFSAGPRNCIGQKFAMLEMKSTISKVLRNFELSVAP
GYEPILVAELILRPDNGVMLILNKRK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
15 g15085.t15 Gene3D G3DSA:1.10.630.10 Cytochrome p450 16 506 1.2E-120
2 g15085.t15 PANTHER PTHR24291:SF143 CYTOCHROME P450 4D1-RELATED 6 505 2.8E-170
3 g15085.t15 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 6 505 2.8E-170
4 g15085.t15 PRINTS PR00463 E-class P450 group I signature 63 82 2.7E-24
9 g15085.t15 PRINTS PR00463 E-class P450 group I signature 325 351 2.7E-24
12 g15085.t15 PRINTS PR00385 P450 superfamily signature 334 347 7.2E-13
7 g15085.t15 PRINTS PR00463 E-class P450 group I signature 370 388 2.7E-24
11 g15085.t15 PRINTS PR00385 P450 superfamily signature 371 382 7.2E-13
6 g15085.t15 PRINTS PR00463 E-class P450 group I signature 410 434 2.7E-24
5 g15085.t15 PRINTS PR00463 E-class P450 group I signature 443 453 2.7E-24
13 g15085.t15 PRINTS PR00385 P450 superfamily signature 444 453 7.2E-13
8 g15085.t15 PRINTS PR00463 E-class P450 group I signature 453 476 2.7E-24
10 g15085.t15 PRINTS PR00385 P450 superfamily signature 453 464 7.2E-13
1 g15085.t15 Pfam PF00067 Cytochrome P450 33 500 1.3E-112
17 g15085.t15 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 5 -
18 g15085.t15 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 6 22 -
16 g15085.t15 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 23 506 -
20 g15085.t15 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 446 455 -
14 g15085.t15 SUPERFAMILY SSF48264 Cytochrome P450 33 505 1.57E-116
19 g15085.t15 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 3 20 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

Pathway

This gene does not belong to any pathways.

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed