General information for g15165.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 305a1.
Parent gene
- The parent gene g15165 is located on chr_4: 4206344..4209675 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15165 | g15165.t2 | TSS | g15165.t2 | 4198659 | 4198659 |
| chr_4 | g15165 | g15165.t2 | isoform | g15165.t2 | 4198660 | 4208087 |
| chr_4 | g15165 | g15165.t2 | exon | g15165.t2.exon1 | 4198660 | 4198762 |
| chr_4 | g15165 | g15165.t2 | exon | g15165.t2.exon2 | 4198811 | 4198938 |
| chr_4 | g15165 | g15165.t2 | cds | g15165.t2.CDS1 | 4198839 | 4198938 |
| chr_4 | g15165 | g15165.t2 | exon | g15165.t2.exon3 | 4207142 | 4207352 |
| chr_4 | g15165 | g15165.t2 | cds | g15165.t2.CDS2 | 4207142 | 4207352 |
| chr_4 | g15165 | g15165.t2 | exon | g15165.t2.exon4 | 4207470 | 4208087 |
| chr_4 | g15165 | g15165.t2 | cds | g15165.t2.CDS3 | 4207470 | 4207818 |
| chr_4 | g15165 | g15165.t2 | TTS | g15165.t2 | NA | NA |
Sequences
>g15165.t2 Gene=g15165 Length=1060
GCTGGTGGCATTTCATTTCAGTAAACGCAACTAAACAGTTGTTATTGAAATAAAAAAAAT
TCATTGTGTGATAAGACAAAAAAACACCCGGAATATATTTTTAAAAAAATAGGTGAAAAT
TTGTTTCAAAAATGTTGGAAATATTTCTTGCTTCTATTGCCTTAATTTTTGTCATTAAGG
CAATTAGTGCAATTTTAGAAGAATTTAGACGCCCTGAAAACTTTCCACCAGGTCCAAACT
GGTTTTTACCATTTGTCGGCAGTTCAAGATACTTTAAAAAATTATGTCAACTATTTGGGG
GACAACATAAAGCTTTTGATTATTTGGCAAAGAAATACAACTCACCAATTTTTAGTATCA
AACTTGGAAGAGAGCTTGTGGTTGTCGTTACAACACATGAATTGATAAAAGAAGTTCATG
CAAGAGAAGAATTTGATGGACGACCAGACAATTTCTTCATACGCCTTCGTACGATGGGAA
CACGTTTAGGTATCACTTCCAGTGATGGTCGCAATTGGCATGAACAAAGAAGTTTTGTTC
TTCGTCAACTTCGAAATGTTGGCTTTGGAAAAACAAAAATGGAAGAGCAAATACATGCAG
AACTCAATGAAATTTTAGATATTATCAAAAGCAAGAAAGACATTCCAATTTGGCCAGGTG
AAAGCAGTTTGATATCAACGAGTATGATAAATATTCTTTGGACTTTCATAACTGGATCAA
AAATCAACGCAAAACAATCCAAATCTAATGAAAATTTTAGAAATATTAGAAAAACGAGGA
AAAGCATTTGATCTCAGTGGTGGATATTTAAATCAAATGCCATGGCTGAGATTCATTGCT
CAGAAGCAACTGGTTATAATTTAATTCAAAATTCAATTCAGCATTTTATGATTTCTTCAT
GGATATAATTAATGATCATATAAAATCATACAGCGATGACAAAACAGGTGATGATGTCAT
TTATGCTTACATCAAGGAAATGAATGCACAAAAAGACAGAGAAAGATACAACATTCAAAC
TTGAACAGCTTGTTTTCATCATTTTGGACATTTTTATAGC
>g15165.t2 Gene=g15165 Length=219
MLEIFLASIALIFVIKAISAILEEFRRPENFPPGPNWFLPFVGSSRYFKKLCQLFGGQHK
AFDYLAKKYNSPIFSIKLGRELVVVVTTHELIKEVHAREEFDGRPDNFFIRLRTMGTRLG
ITSSDGRNWHEQRSFVLRQLRNVGFGKTKMEEQIHAELNEILDIIKSKKDIPIWPGESSL
ISTSMINILWTFITGSKINAKQSKSNENFRNIRKTRKSI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g15165.t2 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 23 | 219 | 2.6E-32 |
| 2 | g15165.t2 | PANTHER | PTHR24303:SF13 | CYTOCHROME P450 305A1-RELATED | 9 | 205 | 5.0E-65 |
| 3 | g15165.t2 | PANTHER | PTHR24303 | HEME-BINDING MONOOXYGENASE FAMILY | 9 | 205 | 5.0E-65 |
| 1 | g15165.t2 | Pfam | PF00067 | Cytochrome P450 | 32 | 207 | 4.4E-19 |
| 8 | g15165.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 9 | g15165.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 10 | g15165.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 11 | g15165.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 19 | - |
| 7 | g15165.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 219 | - |
| 4 | g15165.t2 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 30 | 203 | 1.44E-22 |
| 5 | g15165.t2 | SignalP_EUK | SignalP-TM | SignalP-TM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed