General information for g15191.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 CYP12A2.
Parent gene
- The parent gene g15191 is located on chr_4: 4430134..4432204 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15191 | g15191.t1 | isoform | g15191.t1 | 4430134 | 4432204 |
| chr_4 | g15191 | g15191.t1 | exon | g15191.t1.exon1 | 4430134 | 4430296 |
| chr_4 | g15191 | g15191.t1 | cds | g15191.t1.CDS1 | 4430134 | 4430296 |
| chr_4 | g15191 | g15191.t1 | exon | g15191.t1.exon2 | 4430416 | 4430828 |
| chr_4 | g15191 | g15191.t1 | cds | g15191.t1.CDS2 | 4430416 | 4430828 |
| chr_4 | g15191 | g15191.t1 | exon | g15191.t1.exon3 | 4430884 | 4431043 |
| chr_4 | g15191 | g15191.t1 | cds | g15191.t1.CDS3 | 4430884 | 4431043 |
| chr_4 | g15191 | g15191.t1 | exon | g15191.t1.exon4 | 4431319 | 4431594 |
| chr_4 | g15191 | g15191.t1 | cds | g15191.t1.CDS4 | 4431319 | 4431594 |
| chr_4 | g15191 | g15191.t1 | exon | g15191.t1.exon5 | 4431653 | 4431882 |
| chr_4 | g15191 | g15191.t1 | cds | g15191.t1.CDS5 | 4431653 | 4431882 |
| chr_4 | g15191 | g15191.t1 | exon | g15191.t1.exon6 | 4431935 | 4432204 |
| chr_4 | g15191 | g15191.t1 | cds | g15191.t1.CDS6 | 4431935 | 4432204 |
| chr_4 | g15191 | g15191.t1 | TSS | g15191.t1 | 4432276 | 4432276 |
| chr_4 | g15191 | g15191.t1 | TTS | g15191.t1 | NA | NA |
Sequences
>g15191.t1 Gene=g15191 Length=1512
ATGAAAGCAAAAATTTCACTTTTAAATTTCACTCGTGTTTTTTGTTCAATGAGAAATGCA
GTTCAAAATGCAAAACCATATTCGGAAGTTCCAACTGTGTCAGTTCCAAAATTTATCTTA
AGAAATTTACCTGGTGGAAAATATTATAAAAAATCTTTGAAAGAAATTCAAGAATCATTT
TACAATGAGTTTGGAAATATTTTCAAATTGCCTGCAATGTTTGGCAAACCTGAAATGATT
TTTATTTACAAAGCTGATGACTTTGAAACGACATCACGAGCAGAAGGAGCTTTACCCATT
CGTATGAGCTTTGACACATTGAAATATTACAGGGCGACAAAACAATCTGAAGTTTATGCA
GAATATGGTAGTCTTGCAACAAGTGATGGTGAAAAATGGTACAAAACTCGATCACAAGTT
AATCCTATTATGCTGCAGCCAAAAGTAGTTCAAATGTATACAAAACAAATTGATGAAGTT
TCCAAAGAATTCGTTCAAATTATGCGTGATATAAAAGATGATAAAAATGAAATGCCTAAA
GATTTTAAAGATTACATTAGTAGATGGTCTATTGAATCAATAGCAACAATTGCAGTTGAA
AAACGTTTAAATATTTTGAGTGGAAAAACAGATGATGAAAGAGGCAAAAAATTTATAAAT
GTTACTTTGCAATTTTTCGAAAAATTGTCAGATTTTGAAATGAAACCTTCGATTTGGAGA
TATTATGAGACAAAAGCATTTAAGGAACTTATGGAAGTTTATGATTCAATGACAAGCATT
ATTTTCAGTTATGTCGATGAAGCGATCAAAAGATTTGAAAATGAAGAAACTAAAGGAACT
AATGAGATTCAAGAAGAAAGTGTTCTGAGAAAACTCTTGAAAGTGAACAAAAATCTTGCA
TTTGTGGTTGCTAGTGATTTACTAGTGTCTGACTTGACAACATCTGTTGTTGTATTTACT
CTTTATTTGCTTGCAAAAAATCCTGAAAAACAAGAAAAATTGCGATCAGAATTGATGTCA
ATAATGACAAATAAAAATTCTCCTATAAAATCAGAAAATATGAAAAACTTGCCATATTTG
AGAGCAGTTATAAAAGAGAGTTTGAGAATTTTTCCAATAGGAAGTGGAAATACAAGAAAT
TTATCTAAAGATGTGGTACTTGCTGGTTATCATGTACCAAAAGATACAATGGTCTTCATG
AATGCTTACATTGAACTGTCGAATCCAAAATACTATCCAGAACCCGAAAAATTCATTCCT
GAACGTTGGTTGAGAGATGAAAAGTATGAAAACTGGAGATCAAAAGAAATTAATCCATTT
GCATTTTTGCCTTTCGGATTTGGTGCGAGATCGTGTATTGGAAAACGATTTGGTGAACTT
CAAATAGAAAACATAATTATGAACATCATCAGAAATTTCAAATTGGAGTGGAATTATCCA
GACATGAAAGTCAGAAGTCTTCTTGCAAATTTTCCAGATTCTGATTTAAAATTCAAAATC
ACTGAAGTTTAA
>g15191.t1 Gene=g15191 Length=503
MKAKISLLNFTRVFCSMRNAVQNAKPYSEVPTVSVPKFILRNLPGGKYYKKSLKEIQESF
YNEFGNIFKLPAMFGKPEMIFIYKADDFETTSRAEGALPIRMSFDTLKYYRATKQSEVYA
EYGSLATSDGEKWYKTRSQVNPIMLQPKVVQMYTKQIDEVSKEFVQIMRDIKDDKNEMPK
DFKDYISRWSIESIATIAVEKRLNILSGKTDDERGKKFINVTLQFFEKLSDFEMKPSIWR
YYETKAFKELMEVYDSMTSIIFSYVDEAIKRFENEETKGTNEIQEESVLRKLLKVNKNLA
FVVASDLLVSDLTTSVVVFTLYLLAKNPEKQEKLRSELMSIMTNKNSPIKSENMKNLPYL
RAVIKESLRIFPIGSGNTRNLSKDVVLAGYHVPKDTMVFMNAYIELSNPKYYPEPEKFIP
ERWLRDEKYENWRSKEINPFAFLPFGFGARSCIGKRFGELQIENIIMNIIRNFKLEWNYP
DMKVRSLLANFPDSDLKFKITEV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g15191.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 502 | 1.5E-112 |
| 2 | g15191.t1 | PANTHER | PTHR24291:SF155 | CYTOCHROME P450 301B1 | 43 | 485 | 7.5E-108 |
| 3 | g15191.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 43 | 485 | 7.5E-108 |
| 5 | g15191.t1 | PRINTS | PR00385 | P450 superfamily signature | 145 | 158 | 8.1E-8 |
| 9 | g15191.t1 | PRINTS | PR00465 | E-class P450 group IV signature | 357 | 373 | 4.3E-15 |
| 6 | g15191.t1 | PRINTS | PR00385 | P450 superfamily signature | 362 | 373 | 8.1E-8 |
| 11 | g15191.t1 | PRINTS | PR00465 | E-class P450 group IV signature | 390 | 404 | 4.3E-15 |
| 10 | g15191.t1 | PRINTS | PR00465 | E-class P450 group IV signature | 406 | 424 | 4.3E-15 |
| 12 | g15191.t1 | PRINTS | PR00465 | E-class P450 group IV signature | 436 | 452 | 4.3E-15 |
| 7 | g15191.t1 | PRINTS | PR00385 | P450 superfamily signature | 443 | 452 | 8.1E-8 |
| 4 | g15191.t1 | PRINTS | PR00385 | P450 superfamily signature | 452 | 463 | 8.1E-8 |
| 8 | g15191.t1 | PRINTS | PR00465 | E-class P450 group IV signature | 452 | 470 | 4.3E-15 |
| 1 | g15191.t1 | Pfam | PF00067 | Cytochrome P450 | 39 | 478 | 9.3E-71 |
| 17 | g15191.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 298 | - |
| 18 | g15191.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 299 | 325 | - |
| 16 | g15191.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 326 | 503 | - |
| 14 | g15191.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 445 | 454 | - |
| 13 | g15191.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 37 | 501 | 4.06E-79 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0004497 | monooxygenase activity | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed