General information for g1527.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Maltase A1.
Parent gene
- The parent gene g1527 is located on chr_3: 11601661..11603953 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g1527 | g1527.t1 | isoform | g1527.t1 | 11601661 | 11603953 |
| chr_3 | g1527 | g1527.t1 | exon | g1527.t1.exon1 | 11601661 | 11602719 |
| chr_3 | g1527 | g1527.t1 | cds | g1527.t1.CDS1 | 11601661 | 11602719 |
| chr_3 | g1527 | g1527.t1 | exon | g1527.t1.exon2 | 11602779 | 11602866 |
| chr_3 | g1527 | g1527.t1 | cds | g1527.t1.CDS2 | 11602779 | 11602866 |
| chr_3 | g1527 | g1527.t1 | exon | g1527.t1.exon3 | 11602929 | 11602949 |
| chr_3 | g1527 | g1527.t1 | cds | g1527.t1.CDS3 | 11602929 | 11602949 |
| chr_3 | g1527 | g1527.t1 | exon | g1527.t1.exon4 | 11603061 | 11603185 |
| chr_3 | g1527 | g1527.t1 | cds | g1527.t1.CDS4 | 11603061 | 11603185 |
| chr_3 | g1527 | g1527.t1 | exon | g1527.t1.exon5 | 11603395 | 11603756 |
| chr_3 | g1527 | g1527.t1 | cds | g1527.t1.CDS5 | 11603395 | 11603756 |
| chr_3 | g1527 | g1527.t1 | exon | g1527.t1.exon6 | 11603815 | 11603953 |
| chr_3 | g1527 | g1527.t1 | cds | g1527.t1.CDS6 | 11603815 | 11603953 |
| chr_3 | g1527 | g1527.t1 | TSS | g1527.t1 | NA | NA |
| chr_3 | g1527 | g1527.t1 | TTS | g1527.t1 | NA | NA |
Sequences
>g1527.t1 Gene=g1527 Length=1794
ATGCATCAATTCATTAAAATTTCATTGCTCCTAAGTGCTTTCTACTGCCATAGTGTATAC
ACAGAAGAGTGGTTTGAAACGGGAAATTTCTATCAAATTTATCCACGCTCATTTATGGAC
AGTAATGGTGATGGCATAGGTGACCTCAATGGTATCAAAAGTAAACTTGAATATCTCCAC
GATCTTGGTATGGATGGCGTGTGGCTTTCACCAATTTACAAATCACCAAATGCAGATTTT
GGTTATGACATCTCCGACTTTCGAGATATTCATGAACTTTTTGGTTCACTTGATGATTTT
GATAAACTTGTGCAAGAATGCAATCGACTTGGTTTAAAATTAATTCTTGATTTTGTGCCA
AATCACAGCTCAGATGAGCATGATTGGTTTAAAAGATCTGAAAGTAAAGAAAGTGGATAT
GAGGATTTCTACATTTGGCGTGATCCGAAAATTGATGAGATTTCAAATATTCCAGTTCCA
CCTAACAATTGGATGTCAACTTTTCGATACTCAGCATGGAAATGGTCAGAAGTGAGAAAG
CAAATGTATTATCATATGTTTCATTACAAACAGCCCGATTTAAATTATCGAAATCCAAAA
GTTGTGCAAGAAATGAAAGATGTTTTAACATATTGGCTTGATAGAGGTGTTGCTGGTTTC
AGAATTGATGCTGTTCCTTCACTTTTTGAAGATGAAAGTCTTCGTGATGAACCACGTTCT
TATAGAACTGATTGTGATCAATTTGATCATTGTTCATTACAAAATATTTATACTGAAGAT
CAAAAAGAAACTTATGACATGGTGCAGCAATGGAGAAAACATCTTGATGAATATTCAGCA
GTTAAAAATGTCAGTAAAAAAGTTATTATGGTTGAATCTTATGCAAAACTTGATTACAAT
ATGCTTTATTATGGAAATGAGAGTGCACCTGGAGCACATTTTCCATTTAATTTTGAGTTA
ATTAAAAGAGCAAATATAAATAGTACAGCAGAGGATTACAAATCAATTGTTGAAGAATGG
TTAACTGCAATGCCTAAAGGAAATATCGCTAATTGGGTTCTTGGTAATCATGACAACCAT
CGATTGGCTTCTCGTTTGGGCGAAAAACGTGGCGATTTGTTGAACTTTTTGCTTCAGACG
CTTCCAGGTGCTGCAATAACTTATCAAGGTGAAGAACTTGTTATGAGTGATGTTTATTTA
ACTTGGAATGAAACTGTCGATCCTCAAGCTTGCAATTCTAATCCTACAATTTATGAGTCA
AGATCTCGTGATCCTGCTAGAACTCCTTTTCCTTGGGATAATACAAAAAATGCAGGATTT
TCAACAGCTAATAAAACTTGGCTTCCTGTTGGCAATAATTACAAAACTGTCAATGTTAAA
GCACAACAAGAAGCAAAAGACAGTCACTTGAAAATTTTTAAGAAGCTTACGTCAATCAGG
AAGAAAGAAATTTTCAAAAAAGGCGATTATTTGAGTGCATTGTCAAATAACAAACAAGTT
TATTCATATAAAAGACAATATCAAAATGAAATGGCGATTGTTGTCTTGAATTTTGGAATT
AGTCAAGAAATTGTTAATTTAACTGAACTTTTTAAAGAAATTCCATCACAACTTGAAGTT
TATGTAACATCATTGGATTCTGGAATTATCAGCGGAAGCAAAATTGAAACATCAAAGGTT
AGGATTCCTGGAAGTCATGCAATTATTTTAGTAAAATCAGCAGCTGGAATGATAAAAGCA
AACATTTTAATGATTTTTGTTTCTTCTTTAGTTTATTTTATTTTTAGAAATTGA
>g1527.t1 Gene=g1527 Length=597
MHQFIKISLLLSAFYCHSVYTEEWFETGNFYQIYPRSFMDSNGDGIGDLNGIKSKLEYLH
DLGMDGVWLSPIYKSPNADFGYDISDFRDIHELFGSLDDFDKLVQECNRLGLKLILDFVP
NHSSDEHDWFKRSESKESGYEDFYIWRDPKIDEISNIPVPPNNWMSTFRYSAWKWSEVRK
QMYYHMFHYKQPDLNYRNPKVVQEMKDVLTYWLDRGVAGFRIDAVPSLFEDESLRDEPRS
YRTDCDQFDHCSLQNIYTEDQKETYDMVQQWRKHLDEYSAVKNVSKKVIMVESYAKLDYN
MLYYGNESAPGAHFPFNFELIKRANINSTAEDYKSIVEEWLTAMPKGNIANWVLGNHDNH
RLASRLGEKRGDLLNFLLQTLPGAAITYQGEELVMSDVYLTWNETVDPQACNSNPTIYES
RSRDPARTPFPWDNTKNAGFSTANKTWLPVGNNYKTVNVKAQQEAKDSHLKIFKKLTSIR
KKEIFKKGDYLSALSNNKQVYSYKRQYQNEMAIVVLNFGISQEIVNLTELFKEIPSQLEV
YVTSLDSGIISGSKIETSKVRIPGSHAIILVKSAAGMIKANILMIFVSSLVYFIFRN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g1527.t1 | CDD | cd11328 | AmyAc_maltase | 21 | 490 | 0.0 |
| 8 | g1527.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 24 | 481 | 4.3E-169 |
| 9 | g1527.t1 | Gene3D | G3DSA:3.90.400.10 | - | 124 | 198 | 4.3E-169 |
| 7 | g1527.t1 | Gene3D | G3DSA:2.60.40.1180 | - | 489 | 556 | 9.0E-6 |
| 2 | g1527.t1 | PANTHER | PTHR10357 | ALPHA-AMYLASE FAMILY MEMBER | 8 | 572 | 2.7E-226 |
| 3 | g1527.t1 | PANTHER | PTHR10357:SF175 | MALTASE A1 | 8 | 572 | 2.7E-226 |
| 1 | g1527.t1 | Pfam | PF00128 | Alpha amylase, catalytic domain | 47 | 398 | 4.3E-101 |
| 12 | g1527.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 13 | g1527.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 14 | g1527.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 16 | - |
| 16 | g1527.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 21 | - |
| 11 | g1527.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 573 | - |
| 15 | g1527.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 574 | 595 | - |
| 10 | g1527.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 596 | 597 | - |
| 19 | g1527.t1 | SMART | SM00642 | aamy | 32 | 427 | 1.2E-130 |
| 5 | g1527.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 23 | 482 | 1.17E-124 |
| 4 | g1527.t1 | SUPERFAMILY | SSF51011 | Glycosyl hydrolase domain | 488 | 521 | 1.85E-5 |
| 6 | g1527.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 18 | g1527.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 573 | 595 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K14210 (solute carrier family 3 (neutral and basic amino acid transporter), member 1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed