General information for g15278.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Non-lysosomal glucosylceramidase.
Parent gene
- The parent gene g15278 is located on chr_4: 4782369..4786928 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15278 | g15278.t1 | isoform | g15278.t1 | 4782369 | 4786928 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon1 | 4782369 | 4782996 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS1 | 4782369 | 4782996 |
| chr_4 | g15278 | g15278.t1 | TTS | g15278.t1 | 4782391 | 4782391 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon2 | 4783059 | 4783263 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS2 | 4783059 | 4783263 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon3 | 4783390 | 4783632 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS3 | 4783390 | 4783632 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon4 | 4783694 | 4783814 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS4 | 4783694 | 4783814 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon5 | 4783873 | 4783987 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS5 | 4783873 | 4783987 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon6 | 4784284 | 4784400 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS6 | 4784284 | 4784400 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon7 | 4784492 | 4785274 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS7 | 4784492 | 4785274 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon8 | 4786418 | 4786571 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS8 | 4786418 | 4786571 |
| chr_4 | g15278 | g15278.t1 | exon | g15278.t1.exon9 | 4786637 | 4786928 |
| chr_4 | g15278 | g15278.t1 | cds | g15278.t1.CDS9 | 4786637 | 4786928 |
| chr_4 | g15278 | g15278.t1 | TSS | g15278.t1 | 4787062 | 4787062 |
Sequences
>g15278.t1 Gene=g15278 Length=2658
ATGTCACAAGACGATACATCACAAAGTTTTACTGACTATAAACAGCAAATAACTTCAGTT
CCTGCTTATGGACTAAAGTTTAAGTTCGATCATAAATTTTCGGAAAAAGAAAATTTTACA
CAAAATTTGAAGCCATCATGGAGACAAATATGGAATTTAATGCCAATGATGATGCGATAT
GTGCCATATTATTGGCGTAATAGTAAAGGTGGTAATTTAGTCCTAATGGATTATTTTTCT
ATGCAAAATCATGGTGGAATTCATGGATGTCCAATTGGTGGAATTGGCTCGGGCACAATT
GGACGCGGATTTGCTGGCGAATTCTGTCGGTATCAAATGAAACCGGGATTATATGAGTAT
AATATTGTCCACGCAAATCAATTCATTGTCAACATTCGTGATGAGAATGATAAAACTATT
TTTCAGAGCTTGTTATCGACTTATAGTCGTCCTAAAAATATTCTATCATCATGGGAGAAT
AAACTTGATGCTTCCAAATGCACTTATACAGCTCTCTATCCTCGTTCATGGTCTGAATTT
GATCTCTCTGAATATGGCATTAAGCTCATTTGTAGACAAATTTCTCCAATCATTCCACAT
AATTATAAAGATTCTTCACTTCCGTGTGCTGTCTTCGTTTGGCAAATAGAAAATGTTTGT
GATAAAGAAAGAAAAGTTTCTATAACTTTTACATGGAAAAATGGAACTGGTAATAAAAAA
CAAGATGCTGCCGGTGGAGCTAAAGTGACAACTTTTGATAATGAAGAAATTCGTGGTGCA
ACAATTATGCAAAATATTGTTGATATGCCATGCAATTATCATATTGGCATTTATAAATCA
AGTGATGAAAATTTAAAAGTTGCAACAACTGGTAAAATTGATCCAAATGGAAATGGTAGT
GCTTTTTGGAATGATCTCAATGAAAATGGTCTGCCAACTGAAAAAAGTGATGATAAAAGT
TTAAAAGATGGCAAAGACGTTGCAGTTGCTGTTAATGGTCAAAAAATATTAGCTCCAGGA
GTAATTGATGAACTTGAATTTTCATTAGTTTGGGATATGCCAAAAGTTAGATTTACAAAA
GGCACCAAATGGTTTACAAAATATTATACAAAATATTTTGGTGATGATTTGAATGCTGGT
GAAAAAATTATGGATTATGCATTTAAAAATTATTCAAAATGGGAGCAATTGATTGTTGAT
GAATGGCAGAGACCAATATTAGATGATGAAAGTTTACCTGATTGGTATAAAGCAGCAATT
TTTAATGAATTGTATTTTATTGCTGATGGTGGAAGTCTTTGGTCGATTATTGATAATGAT
GAAAATCTTCCAGTCAGCGATCCAAGGCATGCTTATGGACGCTTTGGTTACCTTGAAGGT
CACGAGTATCGAATGATCAACACCTATGATGTTCATTTTTATGCAAGTCATGCTTTAATC
AGCCTTTGGCCCAATTTACAAGTCAGCATACAGTACGATTTTAAAGACACAATAACAAGA
GAAGTATCAGAAATTCGAAAACATTTGTACGATGGAAAATCATGTGCAAGAAAAATTAAA
AATACAGTTCCACATGATTTAGGTGATCCAGCTGAAGATCCTTGGAATTTAATCAATTCT
TATCCTATTCATGATGTCAGTGAATGGCGTGATCTCAATTCAAAATTCATTCTACAAGTT
TATCGTGATTATTTCGTTCTTAATGAATTTGCACAACTTGATGCTGATAATGCCAGCAAA
TTTAGCTCCATTGAATTCATTGATAAAGAAAGCTTGTCAGAAATGATTATTTTAGACAAT
CGTAACAAAATTGATGATGATAAAAGCAAAAAATCAGCATCAATGTACATCAATGAAACA
AATGGTAAAATTTATTTGATGGATGCTAAGCAATATCTTAGAGATATGTACGAAGTATGC
AAGATTGTCATGGACAAAACAATTGAATTTGATGCTGATAATGATGGAATAATTGAAAAT
TCAAATTCTGCAGATCAAACATACGACACTTGGGTCATGTCCGGACCTAGCATTTATTGT
GGTGGATTATTCTTAGCAAGTCTTCATGTAATGTCAGTAATGGCAAGTATAATGGATGAA
CCAAATGATGCAGTCAAATATCAAGAAATGCTTGAAAAAGGTAAAAAGTCAATTGAAGAA
AAATTATGGAATGGAAATTATTATAAATTTGACACAAGTGGCACAAATAACATCATGTCA
GACCAAATGTGTGGTCATTGGTATCTTCGATGCAGTGGTTTTGATTATGAAATTTTTCCA
AAAGAAAATGTTCGAAAATCATTGAGGACAATTTTTGACAACAATGTAATGAAATTTTGT
GAAGGTCAAATGGGCGCAGTTAATGGTTTTTCACCTGACACAAACAGTCCTGAAACTTTA
AGCATGCAAGCAGAAGAAATTTGGACTGGTGTTGTTTTTGGACTTGCCAGTTGCATGATT
TATGAAGATATGACAGAAGAAGCAATGACAACCGTTTCAGGTCTTTATAAAATGATGACT
GAAAAAGTTGGTTTAGCATTTGAAACACCAGAAGCGATGTATGCTAAAAATAATTATAGA
TCTATTGGTTATATGAGACCATTAAGTATTTATTCAATGCAAACTGCACATGAAAGAAGA
AAAAAGATGAAAAATTAA
>g15278.t1 Gene=g15278 Length=885
MSQDDTSQSFTDYKQQITSVPAYGLKFKFDHKFSEKENFTQNLKPSWRQIWNLMPMMMRY
VPYYWRNSKGGNLVLMDYFSMQNHGGIHGCPIGGIGSGTIGRGFAGEFCRYQMKPGLYEY
NIVHANQFIVNIRDENDKTIFQSLLSTYSRPKNILSSWENKLDASKCTYTALYPRSWSEF
DLSEYGIKLICRQISPIIPHNYKDSSLPCAVFVWQIENVCDKERKVSITFTWKNGTGNKK
QDAAGGAKVTTFDNEEIRGATIMQNIVDMPCNYHIGIYKSSDENLKVATTGKIDPNGNGS
AFWNDLNENGLPTEKSDDKSLKDGKDVAVAVNGQKILAPGVIDELEFSLVWDMPKVRFTK
GTKWFTKYYTKYFGDDLNAGEKIMDYAFKNYSKWEQLIVDEWQRPILDDESLPDWYKAAI
FNELYFIADGGSLWSIIDNDENLPVSDPRHAYGRFGYLEGHEYRMINTYDVHFYASHALI
SLWPNLQVSIQYDFKDTITREVSEIRKHLYDGKSCARKIKNTVPHDLGDPAEDPWNLINS
YPIHDVSEWRDLNSKFILQVYRDYFVLNEFAQLDADNASKFSSIEFIDKESLSEMIILDN
RNKIDDDKSKKSASMYINETNGKIYLMDAKQYLRDMYEVCKIVMDKTIEFDADNDGIIEN
SNSADQTYDTWVMSGPSIYCGGLFLASLHVMSVMASIMDEPNDAVKYQEMLEKGKKSIEE
KLWNGNYYKFDTSGTNNIMSDQMCGHWYLRCSGFDYEIFPKENVRKSLRTIFDNNVMKFC
EGQMGAVNGFSPDTNSPETLSMQAEEIWTGVVFGLASCMIYEDMTEEAMTTVSGLYKMMT
EKVGLAFETPEAMYAKNNYRSIGYMRPLSIYSMQTAHERRKKMKN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g15278.t1 | Gene3D | G3DSA:1.50.10.10 | - | 625 | 784 | 0 |
| 3 | g15278.t1 | PANTHER | PTHR12654:SF0 | NON-LYSOSOMAL GLUCOSYLCERAMIDASE | 11 | 882 | 0 |
| 4 | g15278.t1 | PANTHER | PTHR12654 | BILE ACID BETA-GLUCOSIDASE-RELATED | 11 | 882 | 0 |
| 7 | g15278.t1 | PIRSF | PIRSF028944 | Beta_gluc_GBA2 | 24 | 584 | 0 |
| 8 | g15278.t1 | PIRSF | PIRSF028944 | Beta_gluc_GBA2 | 616 | 883 | 0 |
| 2 | g15278.t1 | Pfam | PF12215 | beta-glucosidase 2, glycosyl-hydrolase family 116 N-term | 89 | 394 | 0 |
| 1 | g15278.t1 | Pfam | PF04685 | Glycosyl-hydrolase family 116, catalytic region | 453 | 873 | 0 |
| 5 | g15278.t1 | SUPERFAMILY | SSF48208 | Six-hairpin glycosidases | 392 | 865 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0006680 | glucosylceramide catabolic process | BP |
| GO:0004348 | glucosylceramidase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K17108 (non-lysosomal glucosylceramidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
