General information for g15460.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g15460 is located on chr_4: 5528966..5532564 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15460 | g15460.t1 | isoform | g15460.t1 | 5528966 | 5532564 |
| chr_4 | g15460 | g15460.t1 | exon | g15460.t1.exon1 | 5528966 | 5529051 |
| chr_4 | g15460 | g15460.t1 | cds | g15460.t1.CDS1 | 5528966 | 5529051 |
| chr_4 | g15460 | g15460.t1 | exon | g15460.t1.exon2 | 5529105 | 5529452 |
| chr_4 | g15460 | g15460.t1 | cds | g15460.t1.CDS2 | 5529105 | 5529452 |
| chr_4 | g15460 | g15460.t1 | exon | g15460.t1.exon3 | 5529646 | 5530203 |
| chr_4 | g15460 | g15460.t1 | cds | g15460.t1.CDS3 | 5529646 | 5530203 |
| chr_4 | g15460 | g15460.t1 | exon | g15460.t1.exon4 | 5530625 | 5530972 |
| chr_4 | g15460 | g15460.t1 | cds | g15460.t1.CDS4 | 5530625 | 5530972 |
| chr_4 | g15460 | g15460.t1 | exon | g15460.t1.exon5 | 5531023 | 5531143 |
| chr_4 | g15460 | g15460.t1 | cds | g15460.t1.CDS5 | 5531023 | 5531143 |
| chr_4 | g15460 | g15460.t1 | exon | g15460.t1.exon6 | 5531245 | 5531387 |
| chr_4 | g15460 | g15460.t1 | cds | g15460.t1.CDS6 | 5531245 | 5531387 |
| chr_4 | g15460 | g15460.t1 | exon | g15460.t1.exon7 | 5531445 | 5531681 |
| chr_4 | g15460 | g15460.t1 | cds | g15460.t1.CDS7 | 5531445 | 5531681 |
| chr_4 | g15460 | g15460.t1 | exon | g15460.t1.exon8 | 5531730 | 5532564 |
| chr_4 | g15460 | g15460.t1 | cds | g15460.t1.CDS8 | 5531730 | 5532564 |
| chr_4 | g15460 | g15460.t1 | TSS | g15460.t1 | NA | NA |
| chr_4 | g15460 | g15460.t1 | TTS | g15460.t1 | NA | NA |
Sequences
>g15460.t1 Gene=g15460 Length=2676
ATGAAGTTTGTAATAGAAAAATATTTTGAATCATCAACAATTCATGGTTTCAAATATTTA
TCAAAGAAATTTCATTGGATTGAAAGAATTTTCTGGATATTTTCACTTTTAACTTCACTA
ATTCTTTCACTTATTCTCATCACAAAACTTGTTATAAAAATCAAAATTGATCCAATAATC
GTTCTTATTCAAGATAATGAAGTTCATGTCAGTGAAATTGATTTCCCAGCTGTAACAGCT
TGTTCAGGTTTAATGCAATTATTACCCGAATATACTTATCATCAAAGACATGAAAGAATA
GAATATGATATGGAATACGAAGATCGTTGGAAAAATATCTTAGATGAAAGACTTTTTGGG
AAATTAAAATTTACAAAATTATTAATGAAACTTGAAAGAAATGAAATCAAAGTTGAAAAT
TTAAGAATGCATTTACTCAAACAACTTCAAGTTATTGATTTGATATCACAACGTGGAACT
TTTGCATTTATAAATTTTTCTATTCCAACAATTGATTTTTTAGATGTCATAAATGAATTT
GATAAAGCAGTTGGCATTGATGAAATTGTTAATTTTCAATGGAGATTTTCAAGTTCTACT
GCAGTTTCAGAAATTTTAACAGAACATGGATTGTGTACAACTTTTAACATAGCTCTACCA
AATGATTTATTCGACACAAATTTGATATCGCAAGATTTTCAATATGCACATATCACTGAA
ATATTTAAAAATTCATCATTTACAGAAGTCATACCAAGAAGAGCCCTAAAATCAAGTCAA
GATGTTTATCTCGATAGTTTCATTGAAAATGAAATGGGTGAAGCAGAAGTTTGGAATTTT
ATAGGAAATTTTATGTATTGGCATGATCCTTATGAGCTACCAACAAAATTTTCACAAACA
AAAATGTTGAGTTATAATAAAATTACAAATATTTGGATTGAACCACAAATTATGGAAATT
GATGAATCTATAGAAAATGATGATTTAAAAGAAGGACAGCAAGGATATGAAATAGTTTCA
AAATATTCACAAATGACTATGAAATATCGGCAAGATTTTGTTAGTAAATTCGTAAGAAGG
AAGCAGTTCGATGAAATTGATTTCTTTTCATTTGTTGGTGGACTTTTGGGATTATTTGCT
GGATTTTCTGCTTTGTCTGCTGTTGAAATTTTTTATTGGTTCATTTTGAAAATTTTTACA
AAAGATTGCAATCGAAGATCAACAGTTGTTTATCCATTTAAAGAAATTGAAGTGAGAAAG
TCAAACATTTTTGAAGATTTCTTCAGTAATTCATCAATTCATGGACTGAGCTATTTAAAG
GACACAAATTTGATTGAAAAACTTTTTTGGACATTTTCTCTAACACTTTCAATTACTATT
TTTGGATTTTTGATGAAAAATATTAAAACACAATCATGGACTACTGCAGTAGATGACAAT
ATGAATTTTAATGAAAAGGTGATACAATTTCCTGCAATTACGTTTTTACCAAGTCGATAT
ATTCACAAAACATTGGTTGATCGTTACATAAATGATGATTTCAACAATGCTAATTTTATA
AAAACTGTTGTGACTAGTTTTAGATCAAAAAAGGAACTTTTTAAGAATATTTCAGCACTT
AGTCAATGTTATATAAATGGTAATTTCACATATGAAAAACTTGGAATAAATTTTAAAAGC
ATTGACAGAATGAGACATGAATCACAAGTTGAATATTTTAAGCAACAGTTTGCAACATGG
AATTACAAATATCAAGTTGATTTTACGGAAGTCAGAACTTATTTTGGCATGGGATTCTCA
TTTAATTTAGTCGATTCTGAGAAACTTTTTAATTACAAAATTGTCAACGATGAATTCAAA
TATAAAAGAAATATTTCAATGACTCAACAAAGTTCAATGCCTAAAATACGAAAGAAATTT
TATCCTTTAATGGTTGACTCAAAATCTCATTCAAAATTCAAACTTATAGTTAAAAAACCA
ACCTTTGATTCTTCAGCATTTTCAAATTGTCGAGAAAATAAAATCAAAGTTCATTCACCT
AATTTTTCTCCAACTTTCATGGAAGATAATGAATTTGCTGAATTAAAGGATTCTGTAAAT
ATCGTTGTGACTTTAAAAATTATAAAAACAGATAGAAATTTACGCTCGTTAAGTCCAATT
GATCGTGATTGTTATTTTGTTGGTGAAAAGAAATTGAAATATTTTAAAATTTATACTCAA
AAATATTGTCAAATGGAATGCGTTATGGACTACATGATTGATTACTGCAAATGTCAAATG
ACAAATTTCATTTACGGAAAGAATAAAAAATTTGAGTTTTGTTATGGTCAAAAACGTTAT
TGTATTTTGCATGAGGAGAGAAATTTTCATCATAAATATGGATATATTTTGGAACACAAT
TGCACTTGTCTACCAGAATGCAATTTTGTAAGTTATCAAACAACTTTTTATCCTGCTGAA
AATTTTGATGACAATGAGACAACAATAATTGTTCAACTTAACACTGATAATTTGATTTTA
TACCGAAAATTTCAACATTTTACATTTTCTGATGCAGTTTCTTATGTTGGTGGTTTGCTT
GGACTTTTTGCTGGAATTTCAATGCTTTCGATTGTTGAAATTTTTTATTTTTTTATTATG
AGAGTTTTTAGTGATGTTTTTAATTGCTGTAATTAA
>g15460.t1 Gene=g15460 Length=891
MKFVIEKYFESSTIHGFKYLSKKFHWIERIFWIFSLLTSLILSLILITKLVIKIKIDPII
VLIQDNEVHVSEIDFPAVTACSGLMQLLPEYTYHQRHERIEYDMEYEDRWKNILDERLFG
KLKFTKLLMKLERNEIKVENLRMHLLKQLQVIDLISQRGTFAFINFSIPTIDFLDVINEF
DKAVGIDEIVNFQWRFSSSTAVSEILTEHGLCTTFNIALPNDLFDTNLISQDFQYAHITE
IFKNSSFTEVIPRRALKSSQDVYLDSFIENEMGEAEVWNFIGNFMYWHDPYELPTKFSQT
KMLSYNKITNIWIEPQIMEIDESIENDDLKEGQQGYEIVSKYSQMTMKYRQDFVSKFVRR
KQFDEIDFFSFVGGLLGLFAGFSALSAVEIFYWFILKIFTKDCNRRSTVVYPFKEIEVRK
SNIFEDFFSNSSIHGLSYLKDTNLIEKLFWTFSLTLSITIFGFLMKNIKTQSWTTAVDDN
MNFNEKVIQFPAITFLPSRYIHKTLVDRYINDDFNNANFIKTVVTSFRSKKELFKNISAL
SQCYINGNFTYEKLGINFKSIDRMRHESQVEYFKQQFATWNYKYQVDFTEVRTYFGMGFS
FNLVDSEKLFNYKIVNDEFKYKRNISMTQQSSMPKIRKKFYPLMVDSKSHSKFKLIVKKP
TFDSSAFSNCRENKIKVHSPNFSPTFMEDNEFAELKDSVNIVVTLKIIKTDRNLRSLSPI
DRDCYFVGEKKLKYFKIYTQKYCQMECVMDYMIDYCKCQMTNFIYGKNKKFEFCYGQKRY
CILHEERNFHHKYGYILEHNCTCLPECNFVSYQTTFYPAENFDDNETTIIVQLNTDNLIL
YRKFQHFTFSDAVSYVGGLLGLFAGISMLSIVEIFYFFIMRVFSDVFNCCN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g15460.t1 | Gene3D | G3DSA:1.10.287.770 | - | 448 | 875 | 3.9E-18 |
| 14 | g15460.t1 | Gene3D | G3DSA:2.60.470.10 | - | 475 | 845 | 3.9E-18 |
| 15 | g15460.t1 | Gene3D | G3DSA:1.10.3590.10 | - | 499 | 672 | 3.9E-18 |
| 16 | g15460.t1 | Gene3D | G3DSA:1.10.287.820 | - | 716 | 807 | 3.9E-18 |
| 6 | g15460.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 5 | 316 | 6.4E-59 |
| 9 | g15460.t1 | PANTHER | PTHR11690:SF237 | NACH, ISOFORM E-RELATED | 5 | 316 | 6.4E-59 |
| 5 | g15460.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 343 | 401 | 6.4E-59 |
| 8 | g15460.t1 | PANTHER | PTHR11690:SF237 | NACH, ISOFORM E-RELATED | 343 | 401 | 6.4E-59 |
| 4 | g15460.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 423 | 883 | 6.4E-59 |
| 7 | g15460.t1 | PANTHER | PTHR11690:SF237 | NACH, ISOFORM E-RELATED | 423 | 883 | 6.4E-59 |
| 1 | g15460.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 8 | 322 | 1.9E-21 |
| 3 | g15460.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 338 | 392 | 3.7E-7 |
| 2 | g15460.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 427 | 877 | 1.6E-45 |
| 21 | g15460.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 29 | - |
| 26 | g15460.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 52 | - |
| 18 | g15460.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 53 | 367 | - |
| 23 | g15460.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 368 | 395 | - |
| 22 | g15460.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 396 | 447 | - |
| 24 | g15460.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 448 | 465 | - |
| 19 | g15460.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 466 | 851 | - |
| 25 | g15460.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 852 | 878 | - |
| 20 | g15460.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 879 | 891 | - |
| 13 | g15460.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 52 | - |
| 12 | g15460.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 374 | 396 | - |
| 11 | g15460.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 448 | 465 | - |
| 10 | g15460.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 857 | 879 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed