General information for g15485.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin BI.
Parent gene
- The parent gene g15485 is located on chr_4: 5611152..5612402 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15485 | g15485.t1 | TSS | g15485.t1 | 5611121 | 5611121 |
| chr_4 | g15485 | g15485.t1 | isoform | g15485.t1 | 5611152 | 5612402 |
| chr_4 | g15485 | g15485.t1 | exon | g15485.t1.exon1 | 5611152 | 5611455 |
| chr_4 | g15485 | g15485.t1 | cds | g15485.t1.CDS1 | 5611152 | 5611455 |
| chr_4 | g15485 | g15485.t1 | exon | g15485.t1.exon2 | 5611587 | 5612015 |
| chr_4 | g15485 | g15485.t1 | cds | g15485.t1.CDS2 | 5611587 | 5612015 |
| chr_4 | g15485 | g15485.t1 | exon | g15485.t1.exon3 | 5612080 | 5612088 |
| chr_4 | g15485 | g15485.t1 | cds | g15485.t1.CDS3 | 5612080 | 5612088 |
| chr_4 | g15485 | g15485.t1 | exon | g15485.t1.exon4 | 5612178 | 5612252 |
| chr_4 | g15485 | g15485.t1 | cds | g15485.t1.CDS4 | 5612178 | 5612252 |
| chr_4 | g15485 | g15485.t1 | exon | g15485.t1.exon5 | 5612311 | 5612402 |
| chr_4 | g15485 | g15485.t1 | cds | g15485.t1.CDS5 | 5612311 | 5612402 |
| chr_4 | g15485 | g15485.t1 | TTS | g15485.t1 | 5612514 | 5612514 |
Sequences
>g15485.t1 Gene=g15485 Length=909
ATGAAATTATTTATTTTTTTAATTTTTTCTATAAGTTCTGCACTTTGTCTTGTGCCAGTG
AAAGAAGGATGGAAATTAGTAAAATCACCTTTAGATAGTCCACATTTTCATGACGTTATG
GAAAAAATTTATTCAAATATTCCTGCAGCAAAACCAAATGTAAGAAGTGGAAGAATTGCT
GGTGGTGAACTTGCTTCTCTTGGTCAATTTCCTCATCTTGTGCTGCTTTACAATGTTGAT
AAATTTGGTGACACTTATATTTGTGGAAGCAGCATTATTAGTCATAATTGGTTACTTACT
GCCGGTCATTGCCTCCATGAAATTTCTTATACAACAGTTTATGCTGGAATTATTAATAGA
ATACTTGGGCCAGCAGTTTGGTCACGAGAAGTCACAGCTGCGTCGGGAAATTTCATTTTC
CATGAAGAATACAAAGGAGTACAAAATGACATCGCTTTAATTCGACTTGTTACTTCAATT
CCAAGTCATCCACATGTTGGAAATATTGCATTACCACGAAGAAGTGATGCAAGTGTTGTT
CTTGATGGAAAAATGGCAACAGTTGCAGGATTTGGAAGAATAAATGATGTTCAAATGCAA
CCATCAGAACATTTAAGATTTATGTCACAACCAATTTATCCAAATAGTAAATGTGAAACG
GTTTTTGGTAAAGTTAATGTTAGAGATACAAATTTATGCTTAAGTGGTGCAAATGGAAAA
TCAACATGTCAAGGTGACTCAGGTGGACCATTGGTAACTGAAATTGCGCCTGGAAGAAAT
GTTTTAGTAGGAATTGTTAGTTTTGGTGCCGAATCAGGTTGCACTTTAGGACATCCACTA
GTGTTCACACGTGTGGCTTCTTATCTTGATTGGATTGAAAAGATAACCGGCATTTATATT
TACGATTGA
>g15485.t1 Gene=g15485 Length=302
MKLFIFLIFSISSALCLVPVKEGWKLVKSPLDSPHFHDVMEKIYSNIPAAKPNVRSGRIA
GGELASLGQFPHLVLLYNVDKFGDTYICGSSIISHNWLLTAGHCLHEISYTTVYAGIINR
ILGPAVWSREVTAASGNFIFHEEYKGVQNDIALIRLVTSIPSHPHVGNIALPRRSDASVV
LDGKMATVAGFGRINDVQMQPSEHLRFMSQPIYPNSKCETVFGKVNVRDTNLCLSGANGK
STCQGDSGGPLVTEIAPGRNVLVGIVSFGAESGCTLGHPLVFTRVASYLDWIEKITGIYI
YD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g15485.t1 | CDD | cd00190 | Tryp_SPc | 59 | 295 | 2.22141E-61 |
| 9 | g15485.t1 | Gene3D | G3DSA:2.40.10.10 | - | 53 | 297 | 3.0E-56 |
| 2 | g15485.t1 | PANTHER | PTHR24250:SF56 | SERINE PROTEASE P96 | 48 | 296 | 1.0E-57 |
| 3 | g15485.t1 | PANTHER | PTHR24250 | CHYMOTRYPSIN-RELATED | 48 | 296 | 1.0E-57 |
| 5 | g15485.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 89 | 104 | 4.1E-14 |
| 4 | g15485.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 146 | 160 | 4.1E-14 |
| 6 | g15485.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 240 | 252 | 4.1E-14 |
| 1 | g15485.t1 | Pfam | PF00089 | Trypsin | 59 | 292 | 2.2E-45 |
| 11 | g15485.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 12 | g15485.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 13 | g15485.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 14 | g15485.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 10 | g15485.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 302 | - |
| 17 | g15485.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 99 | 104 | - |
| 18 | g15485.t1 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 241 | 252 | - |
| 19 | g15485.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 59 | 297 | 26.643 |
| 16 | g15485.t1 | SMART | SM00020 | trypsin_2 | 58 | 292 | 2.5E-66 |
| 7 | g15485.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 53 | 299 | 2.02E-66 |
| 8 | g15485.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
- This transcript has been annotated with K01310 (chymotrypsin)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed