General information for g15485.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin BI.
Parent gene
- The parent gene g15485 is located on chr_4: 5611152..5612402 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15485 | g15485.t3 | TSS | g15485.t3 | 5611121 | 5611121 |
| chr_4 | g15485 | g15485.t3 | isoform | g15485.t3 | 5611152 | 5612402 |
| chr_4 | g15485 | g15485.t3 | exon | g15485.t3.exon1 | 5611152 | 5611455 |
| chr_4 | g15485 | g15485.t3 | cds | g15485.t3.CDS1 | 5611152 | 5611455 |
| chr_4 | g15485 | g15485.t3 | exon | g15485.t3.exon2 | 5611587 | 5612020 |
| chr_4 | g15485 | g15485.t3 | cds | g15485.t3.CDS2 | 5611587 | 5612020 |
| chr_4 | g15485 | g15485.t3 | exon | g15485.t3.exon3 | 5612178 | 5612252 |
| chr_4 | g15485 | g15485.t3 | exon | g15485.t3.exon4 | 5612311 | 5612402 |
| chr_4 | g15485 | g15485.t3 | TTS | g15485.t3 | 5612514 | 5612514 |
Sequences
>g15485.t3 Gene=g15485 Length=905
ATGAAATTATTTATTTTTTTAATTTTTTCTATAAGTTCTGCACTTTGTCTTGTGCCAGTG
AAAGAAGGATGGAAATTAGTAAAATCACCTTTAGATAGTCCACATTTTCATGACGTTATG
GAAAAAATTTATTCAAATATTCCTGCAGCAAAACCAAATGTAAGAAGTGGAAGAATTGCT
GGTGGTGAACTTGCTTCTCTTGGTCAATTTCCTCATCTTGTGCTGCTTTACAATGTTGAT
AAATTTGGTGACACTTATATTTGTGGAAGCAGCATTATTAGTCATAATTGGTTACTTACT
GCCGGTCATTGCCTCCATGAAATTTCTTATACAACAGTTTATGCTGGAATTATTAATAGA
ATACTTGGGCCAGCAGTTTGGTCACGAGAAGTCACAGCTGCGTCGGGAAATTTCATTTTC
CATGAAGAATACAAAGGAGTACAAAATGACATCGCTTTAATTCGACTTGTTACTTCAATT
CCAAGTCATCCACATGTTGGAAATATTGCATTACCACGAAGAAGTGATGCAAGTGTTGTT
CTTGATGGAAAAATGGCAACAGTTGCAGGATTTGGAAGAATAAATGATGTTCAAATGCAA
CCATCAGAACATTTAAGATTTATGTCACAACCAATTTATCCAAATAGTAAATGTGAAACG
GTTTTTGGTAAAGTTAATGTTAGAGATACAAATTTATGCTTAAGTGGTGCAAATGGAAAA
TCAACATGTCAAGGTTAGGTGGACCATTGGTAACTGAAATTGCGCCTGGAAGAAATGTTT
TAGTAGGAATTGTTAGTTTTGGTGCCGAATCAGGTTGCACTTTAGGACATCCACTAGTGT
TCACACGTGTGGCTTCTTATCTTGATTGGATTGAAAAGATAACCGGCATTTATATTTACG
ATTGA
>g15485.t3 Gene=g15485 Length=245
MKLFIFLIFSISSALCLVPVKEGWKLVKSPLDSPHFHDVMEKIYSNIPAAKPNVRSGRIA
GGELASLGQFPHLVLLYNVDKFGDTYICGSSIISHNWLLTAGHCLHEISYTTVYAGIINR
ILGPAVWSREVTAASGNFIFHEEYKGVQNDIALIRLVTSIPSHPHVGNIALPRRSDASVV
LDGKMATVAGFGRINDVQMQPSEHLRFMSQPIYPNSKCETVFGKVNVRDTNLCLSGANGK
STCQG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g15485.t3 | CDD | cd00190 | Tryp_SPc | 59 | 245 | 1.15473E-35 |
| 9 | g15485.t3 | Gene3D | G3DSA:2.40.10.10 | - | 53 | 245 | 1.9E-35 |
| 2 | g15485.t3 | PANTHER | PTHR24260 | - | 54 | 245 | 7.4E-35 |
| 3 | g15485.t3 | PANTHER | PTHR24260:SF90 | AT07769P-RELATED | 54 | 245 | 7.4E-35 |
| 6 | g15485.t3 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 89 | 104 | 3.0E-8 |
| 5 | g15485.t3 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 146 | 160 | 3.0E-8 |
| 4 | g15485.t3 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 240 | 245 | 3.0E-8 |
| 1 | g15485.t3 | Pfam | PF00089 | Trypsin | 59 | 245 | 9.8E-29 |
| 11 | g15485.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 12 | g15485.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 13 | g15485.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 14 | g15485.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 10 | g15485.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 245 | - |
| 17 | g15485.t3 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 99 | 104 | - |
| 18 | g15485.t3 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 59 | 245 | 17.327 |
| 16 | g15485.t3 | SMART | SM00020 | trypsin_2 | 58 | 245 | 2.4E-22 |
| 7 | g15485.t3 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 51 | 245 | 6.63E-43 |
| 8 | g15485.t3 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed