General information for g15577.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative fatty acyl-CoA reductase CG5065.
Parent gene
- The parent gene g15577 is located on chr_4: 5912934..5915015 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15577 | g15577.t1 | TTS | g15577.t1 | 5912731 | 5912731 |
| chr_4 | g15577 | g15577.t1 | isoform | g15577.t1 | 5912934 | 5915015 |
| chr_4 | g15577 | g15577.t1 | exon | g15577.t1.exon1 | 5912934 | 5913114 |
| chr_4 | g15577 | g15577.t1 | cds | g15577.t1.CDS1 | 5912934 | 5913114 |
| chr_4 | g15577 | g15577.t1 | exon | g15577.t1.exon2 | 5913169 | 5913426 |
| chr_4 | g15577 | g15577.t1 | cds | g15577.t1.CDS2 | 5913169 | 5913426 |
| chr_4 | g15577 | g15577.t1 | exon | g15577.t1.exon3 | 5913484 | 5913616 |
| chr_4 | g15577 | g15577.t1 | cds | g15577.t1.CDS3 | 5913484 | 5913616 |
| chr_4 | g15577 | g15577.t1 | exon | g15577.t1.exon4 | 5913674 | 5913836 |
| chr_4 | g15577 | g15577.t1 | cds | g15577.t1.CDS4 | 5913674 | 5913836 |
| chr_4 | g15577 | g15577.t1 | exon | g15577.t1.exon5 | 5913974 | 5914119 |
| chr_4 | g15577 | g15577.t1 | cds | g15577.t1.CDS5 | 5913974 | 5914119 |
| chr_4 | g15577 | g15577.t1 | exon | g15577.t1.exon6 | 5914177 | 5914294 |
| chr_4 | g15577 | g15577.t1 | cds | g15577.t1.CDS6 | 5914177 | 5914294 |
| chr_4 | g15577 | g15577.t1 | exon | g15577.t1.exon7 | 5914376 | 5914510 |
| chr_4 | g15577 | g15577.t1 | cds | g15577.t1.CDS7 | 5914376 | 5914510 |
| chr_4 | g15577 | g15577.t1 | exon | g15577.t1.exon8 | 5914569 | 5915015 |
| chr_4 | g15577 | g15577.t1 | cds | g15577.t1.CDS8 | 5914569 | 5915015 |
| chr_4 | g15577 | g15577.t1 | TSS | g15577.t1 | 5915042 | 5915042 |
Sequences
>g15577.t1 Gene=g15577 Length=1581
ATGTCGAGACAAATTTCAATTCCTGAATTTTATAGTGGAAAATGTGTGTTTATAACAGGT
GGCACTGGATTCATTGGCAAAATGATTGTAGAAAAATTGCTGAGATCTTGTCCAGAAGTT
GATACAATTTATCTTCTTGCAAGAGCTAAAAAGAACAAAACAGCATCAGAACGAGTTCAA
GAAATCACAGATTCAAAACTCTTTGATGTCATTCGTGCAAAAAATCCAGAAGCATTCAAG
AAAATTTCTTTAATTGAAGGCGATATTTCAAATGAAAATTTAGGACTTTCACAATCTAAC
CAAGAACTTCTATGTGAAAAAATCAATGTCATAATTCACTCTGCAGCAACAATTAGCTTC
ACGGAACCAATAAAAGTTGCAGTCGCTACAAATTTGCAATCAGTAAAAGAACTTATGAAT
TTGGCGAGGAAGGTGAAGAAACTTGATTCTTTTGTGCATGTTTCGACAGCTTATACAAAT
TGGTTTGAAAAAGATGTGAAAGAAATATTTTACAAACCAAATTATGATCCAAATAAAGTC
ATTGAAATGTGCAAAACGCTGAGTGATGAAGAAGTAGAAAAGATGATTCCTTCACTTGGA
AAACACAACAACACTTATACGTTTTCTAAATCACTAGCAGAATATCTTATGTCACAAGAG
GGCATTGACTTGCCACTTACGATTGTTAGACCATCAATGGTTATTTCAAGTTTAAATGAA
CCATTTCCTGGATGGATTGATAATTGGGCTGGTCCTTCTCCATTTCTTGTAATGACAGCA
AAAGGTCTTTTTAGACATCCTCATATGAGAAGAGATGTAAAACTTGATGTTATTCCTTCT
GATTTGACTGTCAATATGGTTTTAGCTGCTGGATGGAAGATAGGAACTGATCCAAATGCA
AGAAATTCAGTTCCTGAAATTTACAATTGCACAACAAGCTTTAATAATCCAATATTATGT
AAAGATTTGTTTCAAGGTTTTGTTGATATAGGAAAAAAATATCCATACAGTGACACAATT
TGGTATCCGAAAATAAAATTTTATCATTCAGCTTTGGCTTCTCAATTTTTTTCTTTTGCA
TATCAAAAAATTCCAGCATATTTTGTTGATTTTCTAATGAAACTTGCAGGAAAGAAACCA
AAGCTCATAAAAACTTTAAATTTCGCTTACACAAATTATAACAGTGTCAAATGGGTACCA
ACAAACAACCTGGTCTTTCACAGTCAAAATCCACAAAAAATTTTAAATGTCATGAATCCA
AAAGATCTGCAAGATTTTGATTTTGATGTGAGAAAAATTAATTGGCAAAAATACATCGAA
ATTTATCATTTTGGATTACGAAAGCATTTGGCAAATGAAAAATCAGAAAATTGGCCAGCT
TTGAGAATGAAAGTTCAAAGACTTAAGTACATTCATTATATAGTGACTGGATCGATGATT
GCTGGATCATTGTTTTTACTCTACAAAAGTCGAAATTTAATATCAAATAAAAAAGATGAG
AATCAAGGACAAGGTTTTCAGCAAAAAAGATTACCATCATTTAATGTCATAATAAATAGG
GAGAAATCTTTAAATAAATAA
>g15577.t1 Gene=g15577 Length=526
MSRQISIPEFYSGKCVFITGGTGFIGKMIVEKLLRSCPEVDTIYLLARAKKNKTASERVQ
EITDSKLFDVIRAKNPEAFKKISLIEGDISNENLGLSQSNQELLCEKINVIIHSAATISF
TEPIKVAVATNLQSVKELMNLARKVKKLDSFVHVSTAYTNWFEKDVKEIFYKPNYDPNKV
IEMCKTLSDEEVEKMIPSLGKHNNTYTFSKSLAEYLMSQEGIDLPLTIVRPSMVISSLNE
PFPGWIDNWAGPSPFLVMTAKGLFRHPHMRRDVKLDVIPSDLTVNMVLAAGWKIGTDPNA
RNSVPEIYNCTTSFNNPILCKDLFQGFVDIGKKYPYSDTIWYPKIKFYHSALASQFFSFA
YQKIPAYFVDFLMKLAGKKPKLIKTLNFAYTNYNSVKWVPTNNLVFHSQNPQKILNVMNP
KDLQDFDFDVRKINWQKYIEIYHFGLRKHLANEKSENWPALRMKVQRLKYIHYIVTGSMI
AGSLFLLYKSRNLISNKKDENQGQGFQQKRLPSFNVIINREKSLNK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g15577.t1 | CDD | cd05236 | FAR-N_SDR_e | 14 | 335 | 7.71702E-124 |
| 12 | g15577.t1 | CDD | cd09071 | FAR_C | 361 | 450 | 1.39789E-20 |
| 7 | g15577.t1 | Gene3D | G3DSA:3.40.50.720 | - | 3 | 355 | 5.7E-72 |
| 3 | g15577.t1 | PANTHER | PTHR11011:SF60 | FATTY ACYL-COA REDUCTASE 2 | 6 | 483 | 1.2E-142 |
| 4 | g15577.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 6 | 483 | 1.2E-142 |
| 1 | g15577.t1 | Pfam | PF07993 | Male sterility protein | 18 | 286 | 7.1E-74 |
| 2 | g15577.t1 | Pfam | PF03015 | Male sterility protein | 361 | 453 | 2.9E-21 |
| 9 | g15577.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 469 | - |
| 10 | g15577.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 470 | 488 | - |
| 8 | g15577.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 489 | 526 | - |
| 6 | g15577.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 5 | 264 | 3.61E-31 |
| 5 | g15577.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 470 | 488 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0080019 | fatty-acyl-CoA reductase (alcohol-forming) activity | MF |
KEGG
Orthology
- This transcript has been annotated with K13356 (alcohol-forming fatty acyl-CoA reductase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed