General information for g15704.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Membrane-bound alkaline phosphatase.
Parent gene
- The parent gene g15704 is located on chr_4: 6477484..6479402 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g15704 | g15704.t3 | TSS | g15704.t3 | 6477454 | 6477454 |
| chr_4 | g15704 | g15704.t3 | isoform | g15704.t3 | 6477484 | 6479402 |
| chr_4 | g15704 | g15704.t3 | exon | g15704.t3.exon1 | 6477484 | 6477868 |
| chr_4 | g15704 | g15704.t3 | cds | g15704.t3.CDS1 | 6477484 | 6477868 |
| chr_4 | g15704 | g15704.t3 | exon | g15704.t3.exon2 | 6477966 | 6478145 |
| chr_4 | g15704 | g15704.t3 | cds | g15704.t3.CDS2 | 6477966 | 6478145 |
| chr_4 | g15704 | g15704.t3 | exon | g15704.t3.exon3 | 6478390 | 6479402 |
| chr_4 | g15704 | g15704.t3 | cds | g15704.t3.CDS3 | 6478390 | 6479402 |
| chr_4 | g15704 | g15704.t3 | TTS | g15704.t3 | 6479450 | 6479450 |
Sequences
>g15704.t3 Gene=g15704 Length=1578
ATGAAAATTGAAATTTTCTGCTTGATTTTATTTTTCTCAAATTCATTTTGTGAATTTCGT
TTTCATCCACGTAAGACAAAAGAAGAAATTGAGACCCAAAGAAATGGTCTTGATCCATTA
GGAATAACTCTTGATGATTTTTCTGAAATTTGGATCAAAAGAAGTCAAGATTATATAAAT
GATCGCTTGAATTATCAAAATAACAATGGAAAAGCAAAAAATGTAATTTTCTTCTTGGGC
GATGGACTTTCATTGACAACAACTGCAGCAACTAGAATGTATTTGGGTGGTGAAGAAGTT
GAACTTTCTTATGAAAGATTCCCTCATCTTGGTTTTGTTAAAACTTATTGCGTTGATCGA
CAAGTTGCTGATTCTGCTTGCACTGCAAACGCGTTTTTGCATGGCGTAAAAAATAATTAT
CAAAGTTTAGGATTGACTGCAAATGTTTCTCGAACTGCTTGTACATTCACTGATGAAGAC
ATAACTTATTCAATTGCAAAATGGGCACAAGATGCTGGAAAAGCTACAGGCGTTGTCACA
ACTACTAGAATAACTCATGCTGTAACTGGTGCCTATGCTCATTCACCTCATCGTTCATGG
GAAAATAATGCAGCGATTTCTTCAACTTGTCGAAATGATCCAAATTCTCGTGTCACTGAT
ATTGCTCATCAATTGGTCTACAATGAAGAAGCTCAAAAATTCAAAGTCATTCTTGGTTGT
GGTCGTCGTGAATTTTTAAATACAACAGAAAGAGATGAAGAAAATGGTGCAGGTCGTCGA
ACAGATGGTAGAAATCTCATCAATGAATGGCTTGAAGAGAGAAATAAAGTTGGAAATGCT
CAATATGTTTGGCATAAACAACAATTAGATGAAGTCGATGTTCAATCAACTGATTATTTG
CTTGGTCTTTTTGAAAATGATCATTGCATGTATCGAAATGACATTCGTGATAGAAATTTG
CTTAGACAAGAACCATTATTGACTGATATGACACGAATTGCACTGCAAATGTTACAAAAA
GATGAAAATGGATTTTTCTTGTTAGTCGAAGCAGGAAGAATTGATCATGCACATCATTCT
AATCGTGCACCACAATCAATGGATGAAACTGCTGAATTTGCGAGAGCAATTGAACTGGCA
CGATCATTAACAAGTAGTGAAGATACATTAATTGTTGTATCTTCAGATCATAGTCATGTC
TTCACATATGCTGGCTATCCAGTAAGACGAAATGATATCAACCACATAGCAGGACGTGGA
TCTGATCGTCGACCTTATGAAACACTTTCGTATAGCAATGGACCTTCTGCAACAAATTCA
TTTGATAGTGACGGATATCGCAGAGATATTTCAAATGATATTTTTGGAACTTCAAGAAGA
GCACATCAGTCATTGGTTCCACTTAGCTCTGAAACTCATGCTGGTGAAGATGTTGCTGTC
TATGCGGATGGACCGTGGGCTCAATTATTCCAAGGAAGTTACGAACAAAGTGTTATTCCA
CTTGCAATGGCTTATTCTGCTCACATCGGACCTTATGCTGATTCTAGTCCTATTAATCCT
GGAAAAGAGGAAAAATAG
>g15704.t3 Gene=g15704 Length=525
MKIEIFCLILFFSNSFCEFRFHPRKTKEEIETQRNGLDPLGITLDDFSEIWIKRSQDYIN
DRLNYQNNNGKAKNVIFFLGDGLSLTTTAATRMYLGGEEVELSYERFPHLGFVKTYCVDR
QVADSACTANAFLHGVKNNYQSLGLTANVSRTACTFTDEDITYSIAKWAQDAGKATGVVT
TTRITHAVTGAYAHSPHRSWENNAAISSTCRNDPNSRVTDIAHQLVYNEEAQKFKVILGC
GRREFLNTTERDEENGAGRRTDGRNLINEWLEERNKVGNAQYVWHKQQLDEVDVQSTDYL
LGLFENDHCMYRNDIRDRNLLRQEPLLTDMTRIALQMLQKDENGFFLLVEAGRIDHAHHS
NRAPQSMDETAEFARAIELARSLTSSEDTLIVVSSDHSHVFTYAGYPVRRNDINHIAGRG
SDRRPYETLSYSNGPSATNSFDSDGYRRDISNDIFGTSRRAHQSLVPLSSETHAGEDVAV
YADGPWAQLFQGSYEQSVIPLAMAYSAHIGPYADSSPINPGKEEK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g15704.t3 | CDD | cd16012 | ALP | 73 | 505 | 2.7973E-100 |
| 11 | g15704.t3 | Gene3D | G3DSA:3.40.720.10 | Alkaline Phosphatase | 45 | 516 | 2.4E-161 |
| 2 | g15704.t3 | PANTHER | PTHR11596 | ALKALINE PHOSPHATASE | 44 | 515 | 2.8E-177 |
| 3 | g15704.t3 | PANTHER | PTHR11596:SF85 | AT01495P-RELATED | 44 | 515 | 2.8E-177 |
| 6 | g15704.t3 | PRINTS | PR00113 | Alkaline phosphatase signature | 72 | 92 | 9.5E-39 |
| 5 | g15704.t3 | PRINTS | PR00113 | Alkaline phosphatase signature | 122 | 137 | 9.5E-39 |
| 8 | g15704.t3 | PRINTS | PR00113 | Alkaline phosphatase signature | 169 | 189 | 9.5E-39 |
| 7 | g15704.t3 | PRINTS | PR00113 | Alkaline phosphatase signature | 233 | 243 | 9.5E-39 |
| 4 | g15704.t3 | PRINTS | PR00113 | Alkaline phosphatase signature | 326 | 355 | 9.5E-39 |
| 1 | g15704.t3 | Pfam | PF00245 | Alkaline phosphatase | 72 | 507 | 1.3E-111 |
| 13 | g15704.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 14 | g15704.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 15 | g15704.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 12 | - |
| 16 | g15704.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 12 | g15704.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 525 | - |
| 18 | g15704.t3 | SMART | SM00098 | alk_phosph_2 | 73 | 510 | 5.6E-135 |
| 9 | g15704.t3 | SUPERFAMILY | SSF53649 | Alkaline phosphatase-like | 49 | 512 | 9.92E-127 |
| 10 | g15704.t3 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003824 | catalytic activity | MF |
| GO:0016791 | phosphatase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01077 (alkaline phosphatase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed