Gene loci information

Transcript annotation

  • This transcript has been annotated as Pyruvate kinase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g160 g160.t11 TSS g160.t11 1439458 1439458
chr_3 g160 g160.t11 isoform g160.t11 1440478 1445275
chr_3 g160 g160.t11 exon g160.t11.exon1 1440478 1440694
chr_3 g160 g160.t11 exon g160.t11.exon2 1443302 1443971
chr_3 g160 g160.t11 cds g160.t11.CDS1 1443302 1443971
chr_3 g160 g160.t11 exon g160.t11.exon3 1444037 1444286
chr_3 g160 g160.t11 cds g160.t11.CDS2 1444037 1444286
chr_3 g160 g160.t11 exon g160.t11.exon4 1444354 1444756
chr_3 g160 g160.t11 cds g160.t11.CDS3 1444354 1444651
chr_3 g160 g160.t11 exon g160.t11.exon5 1444811 1444967
chr_3 g160 g160.t11 exon g160.t11.exon6 1445263 1445275
chr_3 g160 g160.t11 TTS g160.t11 1445733 1445733

Sequences

>g160.t11 Gene=g160 Length=1710
TTAGTCGAGACGTAATTTTTAAACAGTTCATTTTGTATTTTCACAGTGTGTGTATCTTGT
TTGCAATTTTGATTCATTCAATTCCAGCTCTCGATTTAGCCGGACATTAGTCAGTGAAAA
TTAAATTAATTTGTGATAAATACTGTTCTGTTCATTAAAAATTGAACCAATCTGTCATTT
ATTCGAGTTATTTATTTAAATTAAATAAGAAATCAAGATGAGCGAAAAATTGCCACAAAT
GAAGGCCGCTGAAGCAATCTCTGCATTGGACCATCTCTGTCAATTGGATCCAAATGCAAA
AACAGGATTTATTCGTTTAACTGGATTGATTTGTACTATCGGACCAGCTTCACGATCACC
CGAAATGTTGGAGAAAATGATGCATTGTGGCATGAACATTGCTCGTATGAATTTCTCACA
TGGAACTCATGAATATCATGCTGGAACTATTAAGAATGTTCGTGAGGCTGTCGATAATTA
TTCGAAAAAATTGGGCTATAAGTATGCTCTTGCAATAGCATTGGATACAAAAGGTCCCGA
AATCCGTACTGGTTTGAATGCTGATAAGGGTGATATTTCATTGTCTCGTGGTGATAAGAT
TAAAGTTACAACTAATAAGGAATGGTTTGAAAAGGGATCTAAGGAACAAATCTATGCTGA
TTATCCAAACATGGTTAAAGTTTTGAAAACAGGTGATCGTATCTTCATTGATGATGGTTT
GATCTCACTCGTTGTCGATTCAATTAATGGCGATACATTGGACTGCACAATTGAAAATGG
TGGTAAATTAGGCCAACAAAAGGGTGTGAATCTTCCAAATGTACCAGTTGATTTACCAGC
TGTTAGTGAGAAAGACAAGTCAGATTTACTTTTTGGTGTTGAACAAGGTGTTGATATGAT
TTTTGCTTCATTCATTCGTGATGGACATGCATTGGATGAAATTCGTAAAATTTTGGGTGA
GGCTGGCAAGAACATTAAAATCATCTCAAAAATTGAGAATCAACAAGGTTTACAAAATAT
TGATGAAATTATTGAGAAATCTGATGGTATTATGGTTGCTCGTGGCGATTTGGGTATTGA
AATTCCACCAGAAAAGGTTTTCCTTGCTCAAAAACAAATTACTGCTCGCTGCAATCGCGC
GCTGAAATCTCAGACGTTGCCAATGCCGTCTTGGATGGTGCTGATTGCACAATGTTGTCA
GGAGAAACTGCTAAGGGCGAGTATCCACTCGAATGTATCACAACTATGGCAAAAACACAA
ATGGAAGCTGAAGCTGCTATTTGGCACAAGAATTTATTCCGTGATTTGGTTTCATTGCAA
CCAACACCAATTGATGCTACACACTCGATTGCTATCTCAGCTGTTGAAGCTGCATCAAAG
ACTCTTGCTTCATGCATCATTTGTCTCACTACATCGGGAAGATCGGCACATTTGATCTCC
AAATATCGCCCACGATGCCCAATCATTACAGTAACACGCTTTGAACAAACTGCACGCCAA
TGTCATCTTTTCCGCGGCATTTTGCCATTGATCTATCAAGATGCTGTTCAAGAAGACTGG
TTGAAAGATGTTGATGCACGTGTCCAATTCGGTATCAAATTTGGAAAATCTCGTGGATTT
ATCAAGAGTGGTGATCCAATTGTCGTTGTTACTGGCTGGAAGTCTGGATCTGGTTTTACA
AACACCTTGCGCGTTTTGAACTGTGAATAA

>g160.t11 Gene=g160 Length=405
MSEKLPQMKAAEAISALDHLCQLDPNAKTGFIRLTGLICTIGPASRSPEMLEKMMHCGMN
IARMNFSHGTHEYHAGTIKNVREAVDNYSKKLGYKYALAIALDTKGPEIRTGLNADKGDI
SLSRGDKIKVTTNKEWFEKGSKEQIYADYPNMVKVLKTGDRIFIDDGLISLVVDSINGDT
LDCTIENGGKLGQQKGVNLPNVPVDLPAVSEKDKSDLLFGVEQGVDMIFASFIRDGHALD
EIRKILGEAGKNIKIISKIENQQGLQNIDEIIEKSDGIMVARGDLGIEIPPEKVFLAQKQ
ITARCNRALKSQTLPMPSWMVLIAQCCQEKLLRASIHSNVSQLWQKHKWKLKLLFGTRIY
SVIWFHCNQHQLMLHTRLLSQLLKLHQRLLLHASFVSLHREDRHI

Protein features from InterProScan

Transcript Database ID Name Start End E.value
11 g160.t11 Gene3D G3DSA:3.40.1380.20 - 16 32 7.4E-129
13 g160.t11 Gene3D G3DSA:3.20.20.60 - 33 310 7.4E-129
12 g160.t11 Gene3D G3DSA:2.40.33.10 - 107 207 7.4E-129
2 g160.t11 PANTHER PTHR11817:SF100 PYRUVATE KINASE 10 310 7.6E-135
3 g160.t11 PANTHER PTHR11817 PYRUVATE KINASE 10 310 7.6E-135
5 g160.t11 PRINTS PR01050 Pyruvate kinase family signature 96 112 2.7E-41
6 g160.t11 PRINTS PR01050 Pyruvate kinase family signature 225 239 2.7E-41
7 g160.t11 PRINTS PR01050 Pyruvate kinase family signature 255 281 2.7E-41
4 g160.t11 PRINTS PR01050 Pyruvate kinase family signature 282 306 2.7E-41
1 g160.t11 Pfam PF00224 Pyruvate kinase, barrel domain 33 310 1.3E-111
10 g160.t11 ProSitePatterns PS00110 Pyruvate kinase active site signature. 253 265 -
9 g160.t11 SUPERFAMILY SSF51621 Phosphoenolpyruvate/pyruvate domain 18 310 1.43E-66
8 g160.t11 SUPERFAMILY SSF50800 PK beta-barrel domain-like 107 206 1.08E-26
14 g160.t11 TIGRFAM TIGR01064 pyruv_kin: pyruvate kinase 34 310 1.3E-108

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0030955 potassium ion binding MF
GO:0000287 magnesium ion binding MF
GO:0004743 pyruvate kinase activity MF
GO:0006096 glycolytic process BP
GO:0003824 catalytic activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values