General information for g160.t12
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Pyruvate kinase.
Parent gene
- The parent gene g160 is located on chr_3: 1444828..1445966 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g160 | g160.t12 | TSS | g160.t12 | 1439458 | 1439458 |
| chr_3 | g160 | g160.t12 | isoform | g160.t12 | 1440478 | 1445966 |
| chr_3 | g160 | g160.t12 | exon | g160.t12.exon1 | 1440478 | 1440694 |
| chr_3 | g160 | g160.t12 | exon | g160.t12.exon2 | 1443302 | 1443971 |
| chr_3 | g160 | g160.t12 | cds | g160.t12.CDS1 | 1443302 | 1443971 |
| chr_3 | g160 | g160.t12 | exon | g160.t12.exon3 | 1444037 | 1444756 |
| chr_3 | g160 | g160.t12 | cds | g160.t12.CDS2 | 1444037 | 1444756 |
| chr_3 | g160 | g160.t12 | exon | g160.t12.exon4 | 1444811 | 1444967 |
| chr_3 | g160 | g160.t12 | cds | g160.t12.CDS3 | 1444811 | 1444967 |
| chr_3 | g160 | g160.t12 | exon | g160.t12.exon5 | 1445263 | 1445966 |
| chr_3 | g160 | g160.t12 | cds | g160.t12.CDS4 | 1445263 | 1445275 |
| chr_3 | g160 | g160.t12 | TTS | g160.t12 | NA | NA |
Sequences
>g160.t12 Gene=g160 Length=2468
TTAGTCGAGACGTAATTTTTAAACAGTTCATTTTGTATTTTCACAGTGTGTGTATCTTGT
TTGCAATTTTGATTCATTCAATTCCAGCTCTCGATTTAGCCGGACATTAGTCAGTGAAAA
TTAAATTAATTTGTGATAAATACTGTTCTGTTCATTAAAAATTGAACCAATCTGTCATTT
ATTCGAGTTATTTATTTAAATTAAATAAGAAATCAAGATGAGCGAAAAATTGCCACAAAT
GAAGGCCGCTGAAGCAATCTCTGCATTGGACCATCTCTGTCAATTGGATCCAAATGCAAA
AACAGGATTTATTCGTTTAACTGGATTGATTTGTACTATCGGACCAGCTTCACGATCACC
CGAAATGTTGGAGAAAATGATGCATTGTGGCATGAACATTGCTCGTATGAATTTCTCACA
TGGAACTCATGAATATCATGCTGGAACTATTAAGAATGTTCGTGAGGCTGTCGATAATTA
TTCGAAAAAATTGGGCTATAAGTATGCTCTTGCAATAGCATTGGATACAAAAGGTCCCGA
AATCCGTACTGGTTTGAATGCTGATAAGGGTGATATTTCATTGTCTCGTGGTGATAAGAT
TAAAGTTACAACTAATAAGGAATGGTTTGAAAAGGGATCTAAGGAACAAATCTATGCTGA
TTATCCAAACATGGTTAAAGTTTTGAAAACAGGTGATCGTATCTTCATTGATGATGGTTT
GATCTCACTCGTTGTCGATTCAATTAATGGCGATACATTGGACTGCACAATTGAAAATGG
TGGTAAATTAGGCCAACAAAAGGGTGTGAATCTTCCAAATGTACCAGTTGATTTACCAGC
TGTTAGTGAGAAAGACAAGTCAGATTTACTTTTTGGTGTTGAACAAGGTGTTGATATGAT
TTTTGCTTCATTCATTCGTGATGGACATGCATTGGATGAAATTCGTAAAATTTTGGGTGA
GGCTGGCAAGAACATTAAAATCATCTCAAAAATTGAGAATCAACAAGGTTTACAAAATAT
TGATGAAATTATTGAGAAATCTGATGGTATTATGGTTGCTCGTGGCGATTTGGGTATTGA
AATTCCACCAGAAAAGGTTTTCCTTGCTCAAAAACAAATTACTGCTCGCTGCAATCGCGC
TGGAAAACCATGTATTTGCGCTACTCAAATGTTGGAGTCAATGACAAACAAGCCACGTGC
TACACGCGCTGAAATCTCAGACGTTGCCAATGCCGTCTTGGATGGTGCTGATTGCACAAT
GTTGTCAGGAGAAACTGCTAAGGGCGAGTATCCACTCGAATGTATCACAACTATGGCAAA
AACACAAATGGAAGCTGAAGCTGCTATTTGGCACAAGAATTTATTCCGTGATTTGGTTTC
ATTGCAACCAACACCAATTGATGCTACACACTCGATTGCTATCTCAGCTGTTGAAGCTGC
ATCAAAGACTCTTGCTTCATGCATCATTTGTCTCACTACATCGGGAAGATCGGCACATTT
GATCTCCAAATATCGCCCACGATGCCCAATCATTACAGTAACACGCTTTGAACAAACTGC
ACGCCAATGTCATCTTTTCCGCGGCATTTTGCCATTGATCTATCAAGATGCTGTTCAAGA
AGACTGGTTGAAAGATGTTGATGCACGTGTCCAATTCGGTATCAAATTTGGAAAATCTCG
TGGATTTATCAAGAGTGGTGATCCAATTGTCGTTGTTACTGGCTGGAAGTCTGGATCTGG
TTTTACAAACACCTTGCGCGTTTTGAACTGTGAATAAGCACATCATCATCGATCGTATTA
ACAAAAAAATTACATCATATATTACTACTACCTATAGCCGATGTCTCTTCTAATCATTGA
ATTAATTTCTCTGTTCCTTAAAGTGCTACATATATGTAGGACAACATCGTTAAGATAGTG
AAAGAAACCTAATAATTTTAATTAACTTCTAAAGTGTTAACAAACACAAACCACCACCGT
TAATAGCAACAGCAACTACAGATCATACAAGAAGAACAGTAAAAATGAAAAGAAAGTGTG
TAACTAATTTCTCATTAGAACAAAAATTCACATTAAAGCGACACAATACATACTATTTAT
TTCAATTTGTTCCCAATTATTACACACTGAAAAGTTGATCTTAAAAGTTTTATGAAAAAC
ATAATTGTATTTATGAACAGAAGATTCAAATTGTTCTTAATGAATAATAATAAATGATGA
AATTATCAATTATTGAAACGATATTTTTTTGTTTTTATATTTTCAGGAATATGATACATT
TTGATCATAAATTTGTGTGGTTGGCGAGTATTGTTTGAGATCATTTTAGTTACTTTTTAA
ATAAATTGAAGACACAACTTGGTTTTCTATATGAAAAATTTAATGCTGATGATTTTATTC
ATGTAGTTTAAGAATTTATGAGACAATAAAACTAATTAATTTTGTAGATACAAGTGCAAA
TTTTCTAT
>g160.t12 Gene=g160 Length=519
MSEKLPQMKAAEAISALDHLCQLDPNAKTGFIRLTGLICTIGPASRSPEMLEKMMHCGMN
IARMNFSHGTHEYHAGTIKNVREAVDNYSKKLGYKYALAIALDTKGPEIRTGLNADKGDI
SLSRGDKIKVTTNKEWFEKGSKEQIYADYPNMVKVLKTGDRIFIDDGLISLVVDSINGDT
LDCTIENGGKLGQQKGVNLPNVPVDLPAVSEKDKSDLLFGVEQGVDMIFASFIRDGHALD
EIRKILGEAGKNIKIISKIENQQGLQNIDEIIEKSDGIMVARGDLGIEIPPEKVFLAQKQ
ITARCNRAGKPCICATQMLESMTNKPRATRAEISDVANAVLDGADCTMLSGETAKGEYPL
ECITTMAKTQMEAEAAIWHKNLFRDLVSLQPTPIDATHSIAISAVEAASKTLASCIICLT
TSGRSAHLISKYRPRCPIITVTRFEQTARQCHLFRGILPLIYQDAVQEDWLKDVDARVQF
GIKFGKSRGFIKSGDPIVVVTGWKSGSGFTNTLRVLNCE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 18 | g160.t12 | CDD | cd00288 | Pyruvate_Kinase | 32 | 516 | 0.0 |
| 15 | g160.t12 | Gene3D | G3DSA:3.40.1380.20 | - | 16 | 515 | 4.3E-236 |
| 17 | g160.t12 | Gene3D | G3DSA:3.20.20.60 | - | 33 | 373 | 4.3E-236 |
| 16 | g160.t12 | Gene3D | G3DSA:2.40.33.10 | - | 107 | 207 | 4.3E-236 |
| 3 | g160.t12 | PANTHER | PTHR11817:SF100 | PYRUVATE KINASE | 10 | 519 | 1.6E-228 |
| 4 | g160.t12 | PANTHER | PTHR11817 | PYRUVATE KINASE | 10 | 519 | 1.6E-228 |
| 7 | g160.t12 | PRINTS | PR01050 | Pyruvate kinase family signature | 96 | 112 | 3.1E-76 |
| 9 | g160.t12 | PRINTS | PR01050 | Pyruvate kinase family signature | 225 | 239 | 3.1E-76 |
| 11 | g160.t12 | PRINTS | PR01050 | Pyruvate kinase family signature | 255 | 281 | 3.1E-76 |
| 6 | g160.t12 | PRINTS | PR01050 | Pyruvate kinase family signature | 282 | 306 | 3.1E-76 |
| 10 | g160.t12 | PRINTS | PR01050 | Pyruvate kinase family signature | 307 | 331 | 3.1E-76 |
| 5 | g160.t12 | PRINTS | PR01050 | Pyruvate kinase family signature | 332 | 350 | 3.1E-76 |
| 8 | g160.t12 | PRINTS | PR01050 | Pyruvate kinase family signature | 351 | 367 | 3.1E-76 |
| 2 | g160.t12 | Pfam | PF00224 | Pyruvate kinase, barrel domain | 33 | 382 | 2.2E-154 |
| 1 | g160.t12 | Pfam | PF02887 | Pyruvate kinase, alpha/beta domain | 399 | 516 | 7.3E-36 |
| 19 | g160.t12 | ProSitePatterns | PS00110 | Pyruvate kinase active site signature. | 253 | 265 | - |
| 14 | g160.t12 | SUPERFAMILY | SSF51621 | Phosphoenolpyruvate/pyruvate domain | 18 | 382 | 6.98E-101 |
| 12 | g160.t12 | SUPERFAMILY | SSF50800 | PK beta-barrel domain-like | 107 | 206 | 1.66E-26 |
| 13 | g160.t12 | SUPERFAMILY | SSF52935 | PK C-terminal domain-like | 388 | 518 | 7.85E-43 |
| 20 | g160.t12 | TIGRFAM | TIGR01064 | pyruv_kin: pyruvate kinase | 34 | 516 | 8.3E-179 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0030955 | potassium ion binding | MF |
| GO:0000287 | magnesium ion binding | MF |
| GO:0004743 | pyruvate kinase activity | MF |
| GO:0006096 | glycolytic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00873 (pyruvate kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed