Gene loci information

Transcript annotation

  • This transcript has been annotated as Pyruvate kinase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g160 g160.t15 TSS g160.t15 1440552 1440552
chr_3 g160 g160.t15 isoform g160.t15 1440648 1445966
chr_3 g160 g160.t15 exon g160.t15.exon1 1440648 1440694
chr_3 g160 g160.t15 exon g160.t15.exon2 1443302 1443971
chr_3 g160 g160.t15 cds g160.t15.CDS1 1443302 1443971
chr_3 g160 g160.t15 exon g160.t15.exon3 1444103 1444657
chr_3 g160 g160.t15 cds g160.t15.CDS2 1444103 1444657
chr_3 g160 g160.t15 exon g160.t15.exon4 1444782 1444967
chr_3 g160 g160.t15 cds g160.t15.CDS3 1444782 1444798
chr_3 g160 g160.t15 exon g160.t15.exon5 1445263 1445966
chr_3 g160 g160.t15 TTS g160.t15 NA NA

Sequences

>g160.t15 Gene=g160 Length=2162
TCTGTCATTTATTCGAGTTATTTATTTAAATTAAATAAGAAATCAAGATGAGCGAAAAAT
TGCCACAAATGAAGGCCGCTGAAGCAATCTCTGCATTGGACCATCTCTGTCAATTGGATC
CAAATGCAAAAACAGGATTTATTCGTTTAACTGGATTGATTTGTACTATCGGACCAGCTT
CACGATCACCCGAAATGTTGGAGAAAATGATGCATTGTGGCATGAACATTGCTCGTATGA
ATTTCTCACATGGAACTCATGAATATCATGCTGGAACTATTAAGAATGTTCGTGAGGCTG
TCGATAATTATTCGAAAAAATTGGGCTATAAGTATGCTCTTGCAATAGCATTGGATACAA
AAGGTCCCGAAATCCGTACTGGTTTGAATGCTGATAAGGGTGATATTTCATTGTCTCGTG
GTGATAAGATTAAAGTTACAACTAATAAGGAATGGTTTGAAAAGGGATCTAAGGAACAAA
TCTATGCTGATTATCCAAACATGGTTAAAGTTTTGAAAACAGGTGATCGTATCTTCATTG
ATGATGGTTTGATCTCACTCGTTGTCGATTCAATTAATGGCGATACATTGGACTGCACAA
TTGAAAATGGTGGTAAATTAGGCCAACAAAAGGGTGTGAATCTTCCAAATGTACCAGTTG
ATTTACCAGCTGTTAGTGAGAAAGACAAGTCAGATTTACTTTTTGGTGTTGAACAAGTGG
GTGAGGCTGGCAAGAACATTAAAATCATCTCAAAAATTGAGAATCAACAAGGTTTACAAA
ATATTGATGAAATTATTGAGAAATCTGATGGTATTATGGTTGCTCGTGGCGATTTGGGTA
TTGAAATTCCACCAGAAAAGGTTTTCCTTGCTCAAAAACAAATTACTGCTCGCTGCAATC
GCGCTGGAAAACCATGTATTTGCGCTACTCAAATGTTGGAGTCAATGACAAACAAGCCAC
GTGCTACACGCGCTGAAATCTCAGACGTTGCCAATGCCGTCTTGGATGGTGCTGATTGCA
CAATGTTGTCAGGAGAAACTGCTAAGGGCGAGTATCCACTCGAATGTATCACAACTATGG
CAAAAACACAAATGGAAGCTGAAGCTGCTATTTGGCACAAGAATTTATTCCGTGATTTGG
TTTCATTGCAACCAACACCAATTGATGCTACACACTCGATTGCTATCTCAGCTGTTGAAG
CTGCATCAAAGACTCTTGCTTCATGCATCATTTGTCTCACTACATCGGGAAGATCGGCAC
ATTTGATCTCCAAACTTCTCATCATTTAATTATATTTGTAGATGCTGTTCAAGAAGACTG
GTTGAAAGATGTTGATGCACGTGTCCAATTCGGTATCAAATTTGGAAAATCTCGTGGATT
TATCAAGAGTGGTGATCCAATTGTCGTTGTTACTGGCTGGAAGTCTGGATCTGGTTTTAC
AAACACCTTGCGCGTTTTGAACTGTGAATAAGCACATCATCATCGATCGTATTAACAAAA
AAATTACATCATATATTACTACTACCTATAGCCGATGTCTCTTCTAATCATTGAATTAAT
TTCTCTGTTCCTTAAAGTGCTACATATATGTAGGACAACATCGTTAAGATAGTGAAAGAA
ACCTAATAATTTTAATTAACTTCTAAAGTGTTAACAAACACAAACCACCACCGTTAATAG
CAACAGCAACTACAGATCATACAAGAAGAACAGTAAAAATGAAAAGAAAGTGTGTAACTA
ATTTCTCATTAGAACAAAAATTCACATTAAAGCGACACAATACATACTATTTATTTCAAT
TTGTTCCCAATTATTACACACTGAAAAGTTGATCTTAAAAGTTTTATGAAAAACATAATT
GTATTTATGAACAGAAGATTCAAATTGTTCTTAATGAATAATAATAAATGATGAAATTAT
CAATTATTGAAACGATATTTTTTTGTTTTTATATTTTCAGGAATATGATACATTTTGATC
ATAAATTTGTGTGGTTGGCGAGTATTGTTTGAGATCATTTTAGTTACTTTTTAAATAAAT
TGAAGACACAACTTGGTTTTCTATATGAAAAATTTAATGCTGATGATTTTATTCATGTAG
TTTAAGAATTTATGAGACAATAAAACTAATTAATTTTGTAGATACAAGTGCAAATTTTCT
AT

>g160.t15 Gene=g160 Length=413
MSEKLPQMKAAEAISALDHLCQLDPNAKTGFIRLTGLICTIGPASRSPEMLEKMMHCGMN
IARMNFSHGTHEYHAGTIKNVREAVDNYSKKLGYKYALAIALDTKGPEIRTGLNADKGDI
SLSRGDKIKVTTNKEWFEKGSKEQIYADYPNMVKVLKTGDRIFIDDGLISLVVDSINGDT
LDCTIENGGKLGQQKGVNLPNVPVDLPAVSEKDKSDLLFGVEQVGEAGKNIKIISKIENQ
QGLQNIDEIIEKSDGIMVARGDLGIEIPPEKVFLAQKQITARCNRAGKPCICATQMLESM
TNKPRATRAEISDVANAVLDGADCTMLSGETAKGEYPLECITTMAKTQMEAEAAIWHKNL
FRDLVSLQPTPIDATHSIAISAVEAASKTLASCIICLTTSGRSAHLISKLLII

Protein features from InterProScan

Transcript Database ID Name Start End E.value
19 g160.t15 Gene3D G3DSA:3.20.20.60 - 33 133 4.0E-29
18 g160.t15 Gene3D G3DSA:2.40.33.10 - 143 207 2.2E-23
20 g160.t15 Gene3D G3DSA:3.20.20.60 - 222 349 2.2E-66
17 g160.t15 Gene3D G3DSA:3.40.1380.20 - 361 411 4.8E-11
3 g160.t15 PANTHER PTHR11817:SF100 PYRUVATE KINASE 10 223 6.1E-173
5 g160.t15 PANTHER PTHR11817 PYRUVATE KINASE 10 223 6.1E-173
4 g160.t15 PANTHER PTHR11817:SF100 PYRUVATE KINASE 224 407 6.1E-173
6 g160.t15 PANTHER PTHR11817 PYRUVATE KINASE 224 407 6.1E-173
8 g160.t15 PRINTS PR01050 Pyruvate kinase family signature 96 112 5.3E-71
7 g160.t15 PRINTS PR01050 Pyruvate kinase family signature 233 259 5.3E-71
11 g160.t15 PRINTS PR01050 Pyruvate kinase family signature 260 284 5.3E-71
10 g160.t15 PRINTS PR01050 Pyruvate kinase family signature 285 309 5.3E-71
12 g160.t15 PRINTS PR01050 Pyruvate kinase family signature 310 328 5.3E-71
9 g160.t15 PRINTS PR01050 Pyruvate kinase family signature 329 345 5.3E-71
1 g160.t15 Pfam PF00224 Pyruvate kinase, barrel domain 33 223 1.0E-65
2 g160.t15 Pfam PF00224 Pyruvate kinase, barrel domain 224 360 1.0E-69
16 g160.t15 ProSitePatterns PS00110 Pyruvate kinase active site signature. 231 243 -
15 g160.t15 SUPERFAMILY SSF51621 Phosphoenolpyruvate/pyruvate domain 18 360 3.21E-90
13 g160.t15 SUPERFAMILY SSF50800 PK beta-barrel domain-like 107 206 1.12E-26
14 g160.t15 SUPERFAMILY SSF52935 PK C-terminal domain-like 339 410 5.49E-10

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0030955 potassium ion binding MF
GO:0000287 magnesium ion binding MF
GO:0004743 pyruvate kinase activity MF
GO:0006096 glycolytic process BP
GO:0003824 catalytic activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed