General information for g160.t22
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Pyruvate kinase.
Parent gene
- The parent gene g160 is located on chr_3: 1444828..1445966 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g160 | g160.t22 | isoform | g160.t22 | 1443300 | 1444123 |
| chr_3 | g160 | g160.t22 | exon | g160.t22.exon1 | 1443300 | 1443971 |
| chr_3 | g160 | g160.t22 | cds | g160.t22.CDS1 | 1443302 | 1443971 |
| chr_3 | g160 | g160.t22 | exon | g160.t22.exon2 | 1444034 | 1444123 |
| chr_3 | g160 | g160.t22 | cds | g160.t22.CDS2 | 1444034 | 1444122 |
| chr_3 | g160 | g160.t22 | TSS | g160.t22 | NA | NA |
| chr_3 | g160 | g160.t22 | TTS | g160.t22 | NA | NA |
Sequences
>g160.t22 Gene=g160 Length=762
AGATGAGCGAAAAATTGCCACAAATGAAGGCCGCTGAAGCAATCTCTGCATTGGACCATC
TCTGTCAATTGGATCCAAATGCAAAAACAGGATTTATTCGTTTAACTGGATTGATTTGTA
CTATCGGACCAGCTTCACGATCACCCGAAATGTTGGAGAAAATGATGCATTGTGGCATGA
ACATTGCTCGTATGAATTTCTCACATGGAACTCATGAATATCATGCTGGAACTATTAAGA
ATGTTCGTGAGGCTGTCGATAATTATTCGAAAAAATTGGGCTATAAGTATGCTCTTGCAA
TAGCATTGGATACAAAAGGTCCCGAAATCCGTACTGGTTTGAATGCTGATAAGGGTGATA
TTTCATTGTCTCGTGGTGATAAGATTAAAGTTACAACTAATAAGGAATGGTTTGAAAAGG
GATCTAAGGAACAAATCTATGCTGATTATCCAAACATGGTTAAAGTTTTGAAAACAGGTG
ATCGTATCTTCATTGATGATGGTTTGATCTCACTCGTTGTCGATTCAATTAATGGCGATA
CATTGGACTGCACAATTGAAAATGGTGGTAAATTAGGCCAACAAAAGGGTGTGAATCTTC
CAAATGTACCAGTTGATTTACCAGCTGTTAGTGAGAAAGACAAGTCAGATTTACTTTTTG
GTGTTGAACAAGAAGGTGTTGATATGATTTTTGCTTCATTCATTCGTGATGGACATGCAT
TGGATGAAATTCGTAAAATTTTGGGTGAGGCTGGCAAGAACA
>g160.t22 Gene=g160 Length=253
MSEKLPQMKAAEAISALDHLCQLDPNAKTGFIRLTGLICTIGPASRSPEMLEKMMHCGMN
IARMNFSHGTHEYHAGTIKNVREAVDNYSKKLGYKYALAIALDTKGPEIRTGLNADKGDI
SLSRGDKIKVTTNKEWFEKGSKEQIYADYPNMVKVLKTGDRIFIDDGLISLVVDSINGDT
LDCTIENGGKLGQQKGVNLPNVPVDLPAVSEKDKSDLLFGVEQEGVDMIFASFIRDGHAL
DEIRKILGEAGKN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g160.t22 | Gene3D | G3DSA:3.20.20.60 | - | 33 | 133 | 0 |
| 8 | g160.t22 | Gene3D | G3DSA:2.40.33.10 | - | 143 | 207 | 0 |
| 2 | g160.t22 | PANTHER | PTHR11817:SF100 | PYRUVATE KINASE | 10 | 252 | 0 |
| 3 | g160.t22 | PANTHER | PTHR11817 | PYRUVATE KINASE | 10 | 252 | 0 |
| 4 | g160.t22 | PRINTS | PR01050 | Pyruvate kinase family signature | 96 | 112 | 0 |
| 5 | g160.t22 | PRINTS | PR01050 | Pyruvate kinase family signature | 226 | 240 | 0 |
| 1 | g160.t22 | Pfam | PF00224 | Pyruvate kinase, barrel domain | 33 | 253 | 0 |
| 7 | g160.t22 | SUPERFAMILY | SSF51621 | Phosphoenolpyruvate/pyruvate domain | 18 | 252 | 0 |
| 6 | g160.t22 | SUPERFAMILY | SSF50800 | PK beta-barrel domain-like | 107 | 206 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0030955 | potassium ion binding | MF |
| GO:0000287 | magnesium ion binding | MF |
| GO:0004743 | pyruvate kinase activity | MF |
| GO:0006096 | glycolytic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
