Gene loci information

Transcript annotation

  • This transcript has been annotated as Pyruvate kinase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g160 g160.t7 TSS g160.t7 1439458 1439458
chr_3 g160 g160.t7 isoform g160.t7 1439839 1443968
chr_3 g160 g160.t7 exon g160.t7.exon1 1439839 1440007
chr_3 g160 g160.t7 exon g160.t7.exon2 1443302 1443968
chr_3 g160 g160.t7 cds g160.t7.CDS1 1443302 1443967
chr_3 g160 g160.t7 TTS g160.t7 NA NA

Sequences

>g160.t7 Gene=g160 Length=836
AGTGATATAAATTTTAAATTAAATTATTAAGTAAAGATAAGATTTCTAAGAAATCGATTC
ATTTAAGTTTAAAGTTGACAAAAGGTTTCATAATAGAATTTCAACAACTTTATTCAAGGT
AGATTGAAAATTCGCAAAAATATTAATTTTATTCATTGGATATACTTGGATGAGCGAAAA
ATTGCCACAAATGAAGGCCGCTGAAGCAATCTCTGCATTGGACCATCTCTGTCAATTGGA
TCCAAATGCAAAAACAGGATTTATTCGTTTAACTGGATTGATTTGTACTATCGGACCAGC
TTCACGATCACCCGAAATGTTGGAGAAAATGATGCATTGTGGCATGAACATTGCTCGTAT
GAATTTCTCACATGGAACTCATGAATATCATGCTGGAACTATTAAGAATGTTCGTGAGGC
TGTCGATAATTATTCGAAAAAATTGGGCTATAAGTATGCTCTTGCAATAGCATTGGATAC
AAAAGGTCCCGAAATCCGTACTGGTTTGAATGCTGATAAGGGTGATATTTCATTGTCTCG
TGGTGATAAGATTAAAGTTACAACTAATAAGGAATGGTTTGAAAAGGGATCTAAGGAACA
AATCTATGCTGATTATCCAAACATGGTTAAAGTTTTGAAAACAGGTGATCGTATCTTCAT
TGATGATGGTTTGATCTCACTCGTTGTCGATTCAATTAATGGCGATACATTGGACTGCAC
AATTGAAAATGGTGGTAAATTAGGCCAACAAAAGGGTGTGAATCTTCCAAATGTACCAGT
TGATTTACCAGCTGTTAGTGAGAAAGACAAGTCAGATTTACTTTTTGGTGTTGAAC

>g160.t7 Gene=g160 Length=222
MSEKLPQMKAAEAISALDHLCQLDPNAKTGFIRLTGLICTIGPASRSPEMLEKMMHCGMN
IARMNFSHGTHEYHAGTIKNVREAVDNYSKKLGYKYALAIALDTKGPEIRTGLNADKGDI
SLSRGDKIKVTTNKEWFEKGSKEQIYADYPNMVKVLKTGDRIFIDDGLISLVVDSINGDT
LDCTIENGGKLGQQKGVNLPNVPVDLPAVSEKDKSDLLFGVE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g160.t7 Gene3D G3DSA:3.20.20.60 - 33 133 0
6 g160.t7 Gene3D G3DSA:2.40.33.10 - 143 207 0
2 g160.t7 PANTHER PTHR11817:SF100 PYRUVATE KINASE 10 222 0
3 g160.t7 PANTHER PTHR11817 PYRUVATE KINASE 10 222 0
1 g160.t7 Pfam PF00224 Pyruvate kinase, barrel domain 33 222 0
5 g160.t7 SUPERFAMILY SSF51621 Phosphoenolpyruvate/pyruvate domain 18 222 0
4 g160.t7 SUPERFAMILY SSF50800 PK beta-barrel domain-like 107 206 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0030955 potassium ion binding MF
GO:0000287 magnesium ion binding MF
GO:0004743 pyruvate kinase activity MF
GO:0006096 glycolytic process BP
GO:0003824 catalytic activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values