Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Gamma-interferon-inducible lysosomal thiol reductase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16024 g16024.t1 isoform g16024.t1 7858629 7859347
chr_4 g16024 g16024.t1 exon g16024.t1.exon1 7858629 7858694
chr_4 g16024 g16024.t1 cds g16024.t1.CDS1 7858629 7858694
chr_4 g16024 g16024.t1 TTS g16024.t1 7858631 7858631
chr_4 g16024 g16024.t1 exon g16024.t1.exon2 7858751 7859347
chr_4 g16024 g16024.t1 cds g16024.t1.CDS2 7858751 7859347
chr_4 g16024 g16024.t1 TSS g16024.t1 7859448 7859448

Sequences

>g16024.t1 Gene=g16024 Length=663
ATGACATTGAAACTCATCACAATTTTATTAGCTTTCTCGGGTTTTTCACAAGCTGCAAAG
CCATTAAAAATCGATGTTTATTTAGAAAGTCTTTGTCCAGCATCCAAAGCATTTATGCAA
GAACAACTTAAACCAAATTACGACGAAATCAAAAATGACGTTTTATTGAAATTTATTCCA
TTTGGAAAATCTCAAAGCACAACAAATTCTTCGGGAACATTCTTTGACTGTCAACATGGA
CGATATGAATGTGAAACCAACATGTTTCAAGCTTGCTCTTTAAATCTCATAGGTGATGAC
CAAGAACGACAAACACAATTTATGATTTGTACAATGGAAACTCAACCTGAAAGATTTCCA
TGTGCTGAAAAAGCTGGATTAAAAATTGATGATGTTAAAAAATGTACAGAAGGTGAAGAA
GGAATTAAATTGCAATTAGAAAATGAAAAATTGACCAAACCAATCATCAATCAAAGTGGT
CATGTGCCATCAATTGTTTTCAATGGAATTTGGACTGAAGAAGATGACGATGCTGCACAA
GAAAATTTTTTGAAAATAGTTCAAGATAAATTGAAAGAAGATTTTGTTATTAAGGAATGG
CTTCTTTATAGGATTTATAAATTTATGGGAATAATAAAAATGTTTAAATTTTTATATTTT
TAA

>g16024.t1 Gene=g16024 Length=220
MTLKLITILLAFSGFSQAAKPLKIDVYLESLCPASKAFMQEQLKPNYDEIKNDVLLKFIP
FGKSQSTTNSSGTFFDCQHGRYECETNMFQACSLNLIGDDQERQTQFMICTMETQPERFP
CAEKAGLKIDDVKKCTEGEEGIKLQLENEKLTKPIINQSGHVPSIVFNGIWTEEDDDAAQ
ENFLKIVQDKLKEDFVIKEWLLYRIYKFMGIIKMFKFLYF

Protein features from InterProScan

Transcript Database ID Name Start End E.value
7 g16024.t1 Gene3D G3DSA:3.40.30.10 Glutaredoxin 14 196 1.8E-6
2 g16024.t1 PANTHER PTHR13234:SF56 GH19763P 8 189 2.2E-41
3 g16024.t1 PANTHER PTHR13234 GAMMA-INTERFERON INDUCIBLE LYSOSOMAL THIOL REDUCTASE GILT 8 189 2.2E-41
1 g16024.t1 Pfam PF03227 Gamma interferon inducible lysosomal thiol reductase (GILT) 23 123 1.3E-25
9 g16024.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 18 -
10 g16024.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 4 -
11 g16024.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 5 13 -
12 g16024.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 18 -
8 g16024.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 19 220 -
4 g16024.t1 SUPERFAMILY SSF52833 Thioredoxin-like 18 185 6.71E-5
6 g16024.t1 SignalP_EUK SignalP-noTM SignalP-noTM 1 18 -
13 g16024.t1 SignalP_GRAM_NEGATIVE SignalP-noTM SignalP-noTM 1 18 -
5 g16024.t1 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM 1 18 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values