General information for g16394.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Chymotrypsin BII.
Parent gene
- The parent gene g16394 is located on chr_4: 9324359..9332549 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16394 | g16394.t1 | TSS | g16394.t1 | 9323582 | 9323582 |
| chr_4 | g16394 | g16394.t1 | isoform | g16394.t1 | 9323612 | 9324864 |
| chr_4 | g16394 | g16394.t1 | exon | g16394.t1.exon1 | 9323612 | 9324054 |
| chr_4 | g16394 | g16394.t1 | cds | g16394.t1.CDS1 | 9323612 | 9324054 |
| chr_4 | g16394 | g16394.t1 | exon | g16394.t1.exon2 | 9324359 | 9324637 |
| chr_4 | g16394 | g16394.t1 | cds | g16394.t1.CDS2 | 9324359 | 9324637 |
| chr_4 | g16394 | g16394.t1 | exon | g16394.t1.exon3 | 9324699 | 9324864 |
| chr_4 | g16394 | g16394.t1 | cds | g16394.t1.CDS3 | 9324699 | 9324864 |
| chr_4 | g16394 | g16394.t1 | TTS | g16394.t1 | 9324913 | 9324913 |
Sequences
>g16394.t1 Gene=g16394 Length=888
ATGAAGTTCTTTCTTTTAACAATTTTTGCAATCGCTGTTGTGTCAGCAGTTGAAGATTAT
AATGCACCAGAATGGCAACCAATTGATTGGGCAAAAGTAATTCCTGCTGATGAGTTGCCA
GGTTTCTGGGAAAATACAGTTTTCGCTCAACTTGCACCAAGATCAAACAGAGAGCGCAGA
ATTGTTGGTGGTCATGAAGCAACACCACATGCTCATCCATATCAAGTCGCTCTGATTCAT
CAACGTGTAATCTTGGCTACAATGTGTGGTGGTTCAGTTATTAGTCCAACTGTTATTCTC
ACAGCTGCTCATTGTCCAATTGGTTCACAATCATCAACTGTCATCACTGGAGCACATAAT
ATGAATGTAATTGAACCAAATCAACAACGTAGAACTGTTCAATCAGCACAATATCGTATT
CATGCTAATTATAATTCACAAAATTTGAATAACGATATCGCTACCATGATCCTTTTGCAA
CCATTGACACTCAACCAATTTGTTGCTGTTATTCCATTGGCACAAGCGAATGCTGGAACA
TTTGCAGGAGTGACTGGACAATCCATTGGATGGGGAAGAACAGTTGATGGTGGTGCTTCG
TCACCAGTTTTGCGAGTTGTTCAAAATCCAATTATCACAAATGCTGTTTGTGCACAAACC
TTTGGAACTGCTGTTGTAAATGCAGCTGTTATATGCACTGATACTACTGGTGGACGTGGA
ACATGTCAAGGTGATTCGGGTGGAGTTCTTTCAGTATCGCAAGGTTCAGGACGTCTTCAA
GTTGGTATTACGAGTTTTGGCAGCAGTGCAGGATGTCAAGCAGGCTTTCCAGCTGGCTAT
GAAAGAGTGAGTGCACAAATCGGATGGATTAACAGCAATATGTCATAA
>g16394.t1 Gene=g16394 Length=295
MKFFLLTIFAIAVVSAVEDYNAPEWQPIDWAKVIPADELPGFWENTVFAQLAPRSNRERR
IVGGHEATPHAHPYQVALIHQRVILATMCGGSVISPTVILTAAHCPIGSQSSTVITGAHN
MNVIEPNQQRRTVQSAQYRIHANYNSQNLNNDIATMILLQPLTLNQFVAVIPLAQANAGT
FAGVTGQSIGWGRTVDGGASSPVLRVVQNPIITNAVCAQTFGTAVVNAAVICTDTTGGRG
TCQGDSGGVLSVSQGSGRLQVGITSFGSSAGCQAGFPAGYERVSAQIGWINSNMS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g16394.t1 | CDD | cd00190 | Tryp_SPc | 61 | 293 | 2.82607E-69 |
| 9 | g16394.t1 | Gene3D | G3DSA:2.40.10.10 | - | 54 | 295 | 4.6E-57 |
| 2 | g16394.t1 | PANTHER | PTHR24250:SF56 | SERINE PROTEASE P96 | 9 | 292 | 3.1E-81 |
| 3 | g16394.t1 | PANTHER | PTHR24250 | CHYMOTRYPSIN-RELATED | 9 | 292 | 3.1E-81 |
| 6 | g16394.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 90 | 105 | 4.0E-10 |
| 5 | g16394.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 148 | 162 | 4.0E-10 |
| 4 | g16394.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 239 | 251 | 4.0E-10 |
| 1 | g16394.t1 | Pfam | PF00089 | Trypsin | 61 | 290 | 2.6E-49 |
| 11 | g16394.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 12 | g16394.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 13 | g16394.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 14 | g16394.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 10 | g16394.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 295 | - |
| 17 | g16394.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 100 | 105 | - |
| 18 | g16394.t1 | ProSitePatterns | PS00135 | Serine proteases, trypsin family, serine active site. | 240 | 251 | - |
| 19 | g16394.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 61 | 295 | 26.969 |
| 16 | g16394.t1 | SMART | SM00020 | trypsin_2 | 60 | 290 | 5.3E-67 |
| 7 | g16394.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 53 | 294 | 2.65E-63 |
| 8 | g16394.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
- This transcript has been annotated with K01350 (myeloblastin)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed