Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Glutathione S-transferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16418 g16418.t43 TTS g16418.t43 9389373 9389373
chr_4 g16418 g16418.t43 isoform g16418.t43 9389376 9392769
chr_4 g16418 g16418.t43 exon g16418.t43.exon1 9389376 9389481
chr_4 g16418 g16418.t43 cds g16418.t43.CDS1 9389378 9389481
chr_4 g16418 g16418.t43 exon g16418.t43.exon2 9389547 9389843
chr_4 g16418 g16418.t43 cds g16418.t43.CDS2 9389547 9389843
chr_4 g16418 g16418.t43 exon g16418.t43.exon3 9391085 9391252
chr_4 g16418 g16418.t43 cds g16418.t43.CDS3 9391085 9391252
chr_4 g16418 g16418.t43 exon g16418.t43.exon4 9392584 9392769
chr_4 g16418 g16418.t43 cds g16418.t43.CDS4 9392584 9392716
chr_4 g16418 g16418.t43 TSS g16418.t43 9392764 9392764

Sequences

>g16418.t43 Gene=g16418 Length=757
ACGTCAGTACAGCGTTAGATTTCGAATAGTTCGCATCGTTTTTATATTGAACGATGCCAA
GTTATAAAGTCAGTTATTTTACATTCAAAGGTCTTGGAGAGCCAGTTCGATTTATGCTGG
CTTATGCAAACGTTGATTTCATCGATAATCGTGTTGAGTGGGAAGATTGGCCAAAACTTA
AGCCAACTCTTCCACTTGGCCAACTTCCACTACTTGAGATTGATGGACGAGCATTTCATC
ATTGCATTCCAATTTGCCGGTATCTTGGCAGTATTTTTAATCTCACAGGCAGTAACGCAG
TGGAAAATTATGAAATTGATTGCATAGCGGATACAGTGAATGAATTGCGCTTGAAAATAG
CAATGTGGTACTACAGTTACAAACGAGTAAAAAATGAAAAATATGATGAACTTATCAATG
ATTCAATTCCATATTATCTCGGTAAAATAGAAGATCAAGCAGCAACAAAGGATGGATATT
TAGCGCTTAAAGGAAAGACAACATGGGCAGATATTTATGCAGTTGCAATATTTGATTACA
TACATGACTTAATGGGCTATGATATCGTAAAGGATTGTAAAAATATCAAAAAAATTCAAC
AAAAAATTATGTCAGCTGATGGAGTAAAAAGATATCTAAAAAATCGTCCAGAATTTTGTG
ATTTCTTTTTAATGTTGTGGTTTATTTTTTTAAGTTTCTTTTTTCGTTTTACAAATACAA
ATAAAAAATTTTTTGTATCAATGTGTAGAATAAAAAA

>g16418.t43 Gene=g16418 Length=234
MPSYKVSYFTFKGLGEPVRFMLAYANVDFIDNRVEWEDWPKLKPTLPLGQLPLLEIDGRA
FHHCIPICRYLGSIFNLTGSNAVENYEIDCIADTVNELRLKIAMWYYSYKRVKNEKYDEL
INDSIPYYLGKIEDQAATKDGYLALKGKTTWADIYAVAIFDYIHDLMGYDIVKDCKNIKK
IQQKIMSADGVKRYLKNRPEFCDFFLMLWFIFLSFFFRFTNTNKKFFVSMCRIK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
12 g16418.t43 CDD cd03039 GST_N_Sigma_like 4 72 3.89842E-25
11 g16418.t43 CDD cd03192 GST_C_Sigma_like 83 185 7.51393E-18
7 g16418.t43 Gene3D G3DSA:3.40.30.10 Glutaredoxin 4 196 1.8E-56
6 g16418.t43 Gene3D G3DSA:1.20.1050.10 - 76 187 1.8E-56
2 g16418.t43 PANTHER PTHR11571:SF234 GLUTATHIONE S-TRANSFERASE S1 2 200 1.6E-58
3 g16418.t43 PANTHER PTHR11571 GLUTATHIONE S-TRANSFERASE 2 200 1.6E-58
1 g16418.t43 Pfam PF14497 Glutathione S-transferase, C-terminal domain 97 198 8.5E-13
8 g16418.t43 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 1 200 -
10 g16418.t43 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 201 220 -
9 g16418.t43 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 221 234 -
15 g16418.t43 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile. 2 79 22.613
14 g16418.t43 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile. 81 203 14.307
16 g16418.t43 SFLD SFLDG01205 AMPS.1 1 200 0.0
17 g16418.t43 SFLD SFLDS00019 Glutathione Transferase (cytosolic) 1 200 0.0
4 g16418.t43 SUPERFAMILY SSF52833 Thioredoxin-like 2 75 1.48E-16
5 g16418.t43 SUPERFAMILY SSF47616 GST C-terminal domain-like 77 199 6.82E-20
13 g16418.t43 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 201 220 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006749 glutathione metabolic process BP
GO:0005515 protein binding MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values