Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Glutathione S-transferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16419 g16419.t7 isoform g16419.t7 9392810 9395632
chr_4 g16419 g16419.t7 exon g16419.t7.exon1 9392810 9393198
chr_4 g16419 g16419.t7 TTS g16419.t7 9392821 9392821
chr_4 g16419 g16419.t7 cds g16419.t7.CDS1 9392882 9393198
chr_4 g16419 g16419.t7 exon g16419.t7.exon2 9394140 9394307
chr_4 g16419 g16419.t7 cds g16419.t7.CDS2 9394140 9394307
chr_4 g16419 g16419.t7 exon g16419.t7.exon3 9395516 9395632
chr_4 g16419 g16419.t7 cds g16419.t7.CDS3 9395516 9395519
chr_4 g16419 g16419.t7 TSS g16419.t7 9395732 9395732

Sequences

>g16419.t7 Gene=g16419 Length=674
ATGGTGGTTTACAAATTACATTATTTTAATTTAACTGGTTTGGGTGAACCGATTCGATTT
TTATTCCATTATGGTGGAATTGATTTTGAAGATGTGAGGTATGAAATGAATGAATGGAAT
TCCCTCTTGGTCAATTACCAGTCTTAGAAATAGACGACAAAGAGCATGTTCAATCAATGG
CAATTTGTCGCTATTTAGCAAAACAAGTCGGTCTTGCTGGAGACAGTGACCTTGAAAACA
TGGAAATTGATGCGGTTGTTGATACTTTCAATGATATTCGTCTTAAAATTTCAACTGTGA
TGTGGGAACAAAATCAGGAAGTAAAAAAAGAGAAACAAAAAATTCTACACGAGCAGCAAA
TTCCATTTTATTTTAAGAAGCTTGATGAATATGCCGAAGCCAATAATGGACATTTTGCAT
GTAAAAAATTAACTTGGGCTGACTTGTACATCGCATCTTGGTCAAAGTATTTCTCTTATG
CCATCAAAGAAGTCTTCAATGATTATGAAAACTATCCAAACATTAAGAAAGTTGTCGACA
ATGTTTTAGCAATTGAAAACATTAAAAAATGGGTTGAAACAAGACCCGACACATTCTGTT
GAAAGGAAATTTTTTTTCGCGAATAAATTCTTAATAAAACGAATACGCACTAATGTTTTT
TGTTTACATGGAAA

>g16419.t7 Gene=g16419 Length=162
MEFPLGQLPVLEIDDKEHVQSMAICRYLAKQVGLAGDSDLENMEIDAVVDTFNDIRLKIS
TVMWEQNQEVKKEKQKILHEQQIPFYFKKLDEYAEANNGHFACKKLTWADLYIASWSKYF
SYAIKEVFNDYENYPNIKKVVDNVLAIENIKKWVETRPDTFC

Protein features from InterProScan

Transcript Database ID Name Start End E.value
11 g16419.t7 CDD cd03192 GST_C_Sigma_like 41 144 0.0000000
8 g16419.t7 Gene3D G3DSA:3.40.30.10 Glutaredoxin 1 37 0.0000000
7 g16419.t7 Gene3D G3DSA:1.20.1050.10 - 38 146 0.0000000
3 g16419.t7 PANTHER PTHR11571:SF234 GLUTATHIONE S-TRANSFERASE S1 2 160 0.0000000
4 g16419.t7 PANTHER PTHR11571 GLUTATHIONE S-TRANSFERASE 2 160 0.0000000
1 g16419.t7 Pfam PF02798 Glutathione S-transferase, N-terminal domain 2 29 0.0000150
2 g16419.t7 Pfam PF14497 Glutathione S-transferase, C-terminal domain 53 157 0.0000000
10 g16419.t7 ProSiteProfiles PS50404 Soluble glutathione S-transferase N-terminal domain profile. 1 36 15.6260000
9 g16419.t7 ProSiteProfiles PS50405 Soluble glutathione S-transferase C-terminal domain profile. 38 162 17.5010000
12 g16419.t7 SFLD SFLDS00019 Glutathione Transferase (cytosolic) 3 146 0.0000000
5 g16419.t7 SUPERFAMILY SSF52833 Thioredoxin-like 3 32 0.0000001
6 g16419.t7 SUPERFAMILY SSF47616 GST C-terminal domain-like 34 160 0.0000000

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006749 glutathione metabolic process BP
GO:0005515 protein binding MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values