Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Pyrroline-5-carboxylate reductase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g1643 g1643.t8 TTS g1643.t8 12273050 12273050
chr_3 g1643 g1643.t8 isoform g1643.t8 12273130 12274118
chr_3 g1643 g1643.t8 exon g1643.t8.exon1 12273130 12273422
chr_3 g1643 g1643.t8 cds g1643.t8.CDS1 12273130 12273422
chr_3 g1643 g1643.t8 exon g1643.t8.exon2 12273482 12274118
chr_3 g1643 g1643.t8 cds g1643.t8.CDS2 12273482 12273650
chr_3 g1643 g1643.t8 TSS g1643.t8 12274192 12274192

Sequences

>g1643.t8 Gene=g1643 Length=930
ATGGAGGACATTATAAAATACAAAAATTCATTCTCAACTGAGGCAAAGCTTGGTTTCATA
GGATGTGGCAATATGGCATTTTCAATAGTTAAGGGTTTGCTGCAATCTAAATTATTTGAG
GCAAATGAAATAATAGTTTCTGGTACAAGAGATGAGAGCTTTGAGAGATTGAAGTAAGTT
ATATAGCATTCATTAGTATGTGTATAATAATATTCATATTTTAGGTCATTAGGAAAAATT
AATACAACAAAAAACAATAATGAGGTTGTAAGAGCGGCAGCTATAATTTTTTTGTGTGTC
AAGCCTGCTCATTTGGATGCTGTTGCAATTTCTCTCAAAAAGAATTTCCTTAATCCCGAT
GAAATAGATTTGTCTAATTTAAATAAGACACTTGTTTCTATTCTTGCTGGAACATCATTG
AATACTCTTAGAGAAGCAATTCCAAATTTTGGATCATATATACGTGTTATGCCTAACACT
CCGCTTCAAGTAGGAGCCGGATGTTCTGCAATAACTATTTTTAATGCTTCACTAGATCTT
TTAAATAGTTATAACGCAGTAAAAGCTATTTTTTGCTCACTTGGTCTTGTGGATGTCCTT
GATGAAAGCAAATTTCATGCTATAACTGCTCTTTCAGGAAGTGGGCCAGCTTATGTCTAC
ATAATAATTGATGCCTTAAGTGATGCTGGTGTTAAACAAGGTCTTCCGAGAGATATTGCC
ACTCGTTTTGCAGCACAAACACTTCTTGGTGCCTCAAAAACAGTCTTAGAAAGTTCATTT
CACGTTGGTCAATTAAAAGATCAAGTAACAAGCAGTGGTGGTACAACAATTCATGCCATA
CATGAACTTGAAAAAGGTGGAATACGAAATACACTATTCAATGCAATTGAAGCTGCAACA
AAGCGCTCAAAGGAAATGTCAGAAAATTAA

>g1643.t8 Gene=g1643 Length=153
MPNTPLQVGAGCSAITIFNASLDLLNSYNAVKAIFCSLGLVDVLDESKFHAITALSGSGP
AYVYIIIDALSDAGVKQGLPRDIATRFAAQTLLGASKTVLESSFHVGQLKDQVTSSGGTT
IHAIHELEKGGIRNTLFNAIEAATKRSKEMSEN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
5 g1643.t8 Gene3D G3DSA:1.10.3730.10 - 43 153 0
2 g1643.t8 PANTHER PTHR11645 PYRROLINE-5-CARBOXYLATE REDUCTASE 1 151 0
3 g1643.t8 PANTHER PTHR11645:SF0 PYRROLINE-5-CARBOXYLATE REDUCTASE 2 1 151 0
1 g1643.t8 Pfam PF14748 Pyrroline-5-carboxylate reductase dimerisation 46 150 0
4 g1643.t8 SUPERFAMILY SSF48179 6-phosphogluconate dehydrogenase C-terminal domain-like 45 152 0

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

## [1] "No matching GO terms"

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values