General information for g16433.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine/threonine-protein kinase/endoribonuclease IRE1.
Parent gene
- The parent gene g16433 is located on chr_4: 9426103..9426659 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16433 | g16433.t1 | TTS | g16433.t1 | 9422557 | 9422557 |
| chr_4 | g16433 | g16433.t1 | isoform | g16433.t1 | 9422579 | 9426659 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon1 | 9422579 | 9422980 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS1 | 9422579 | 9422980 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon2 | 9423048 | 9423515 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS2 | 9423048 | 9423515 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon3 | 9423574 | 9423612 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS3 | 9423574 | 9423612 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon4 | 9423670 | 9424199 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS4 | 9423670 | 9424199 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon5 | 9424270 | 9424673 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS5 | 9424270 | 9424673 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon6 | 9424841 | 9425066 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS6 | 9424841 | 9425066 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon7 | 9425804 | 9425925 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS7 | 9425804 | 9425925 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon8 | 9426017 | 9426092 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS8 | 9426017 | 9426092 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon9 | 9426157 | 9426523 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS9 | 9426157 | 9426523 |
| chr_4 | g16433 | g16433.t1 | exon | g16433.t1.exon10 | 9426582 | 9426659 |
| chr_4 | g16433 | g16433.t1 | cds | g16433.t1.CDS10 | 9426582 | 9426659 |
| chr_4 | g16433 | g16433.t1 | TSS | g16433.t1 | 9426727 | 9426727 |
Sequences
>g16433.t1 Gene=g16433 Length=2712
ATGAAAATCAATTTCAAGTTTTTAATTTTTATTTTTATCTTTCAATGGATAAATTTGGGT
AAAACTGATGATGATTTGAGAAAAATCGCAAGATCAGATGAACCATTAATGGTTCTTGTG
ACACTCTCAGGATCAATGATTGGAATCTGTCCACAAACAGCAACACAGAGATGGATAAAA
CAACATGATCCAATTGTCAAAACAAATGGAAATGAAAATTTAAATTTTTCAACAATTTTA
TTACCTGATCCAGTGACTGGAAAATTGTATAACATGAAAACTAGCGATAAAGACAAATAT
GAACTTTCACAAATGGATTTTACAATTCCTGACTTGGTACTTAAAACTCCTTTTATTTCT
AAGGATAAAATTTTATACACTGGAAGAAAATTGGATTCTTGGTTTATGATTAACTCACTA
ACTGGTGAATGCAAGACTGTTATGGATTTTGATGATAACAAAGATAAAAAGACAACAGAT
TTAATACCATTTCCAACAATTCATGATGTCTACATAGGAAAAACACAATATGATGTTTGT
ATGACAAATATGTACCCTGATAACAAAACTAGAGAAATATGGAATGTTTCCATGTATCAG
TACAATGCATTTGAAATGAATAAAGAAATTTATGACAAATATGAATTCAATCACATTGTC
AGCAGTTCAAGTGGGAAAATTCTAACACTTTTTAAAACAACAGGAAAAATTTTCTGGCAT
AAAACAGATTTGAATTCACCAGTCGTTGCCTTATTTATGATGGGTAGAAACGGATTTTTA
AACATTCCATTTACTACAGTTGATGATTCAGTATTTGAAAAGCTTTTGGAGCCTGTTGAT
TATGATAAATTAAAAGATATTCAATTACTTGAAACAATTTTTATCGGTGAAAATAAAAGT
GCAGGCTCAAATTCATTTTATGCTTTACCAACATTATCAGACAATACAACATTTATAATT
CGTGACAAAGTGATAAATTTTTTAAAAGATCCTTCATCAATTATTGGTCATTATTTATTA
CCATCATCAAATTCAGCTGAAACAAATTCAAAATTAATCACTGATGAATCACAAAGCAAT
AAATCTACAGAACTAAAAAATGAAAACAAAAATGAAAAAATCGCAGTGATTTTAGCATTG
GTTGTAATTTTAATTTCCTTGGTGATAACTTTTGGCATAATTGTGATAAAAAGAAAATTA
AAAAATGAAACAAAAAAGAATAATGTCAGTGAAAGCGATGAACAAATCGTCGTGGATTCA
AAAATAGATGAAGATGGACGAACAGTGATATCAAAAATAAAATTTCATCGCAATGATCTC
ATTGGTCGTGGAAGTGAAGGAACTTCAGTATTTAAAGGCACGTTTGAAGGTCGTGAAGTT
GCAGTCAAACGTCTTCTTACCAGTAATTTTTCACTTGCTGAACGCGAAGTTAAAATTCTT
CAAAAATCAGATGCAAATGAAAACATCATCCGATATTTTTGCACAGAATTTGATCGTGAA
TTTTGCTACATTGCAATTGAACTTTGTGCATGTTCACTTTATGATTTTACAGTAAATGAA
GATTTTCAATATTTGAAAGATTTAATCAAACCAAAAGAAGTTCTTTATCAAGCTACAAAA
GGATTGTCTTATTTACATCAATTAGGGATTATTCATAGAGATATAAAACCACAGAATATT
TTAATTTCTAAACCAGATGGTAGAAAAAATATTAAAGTCATGATTTCTGATTTTGGTCTT
TGTAAAAAATTTTCTAAAGGAGAAATTTCTGTAACAAATAGAATTGGAATTGCAGGAACT
GAGGGTTGGATTGCACCTGAAATTCTCATGAATGAAAAATCGTCAAAAATCGCAGATATT
TTTTCACTTGGTTGTGTTTTTTATTTCACACTCACAAATGGTCAACATCCTTTTGGTGAT
TCATTAACAAGAACTTCAAACATTCTTGCCAATAATTTCAATCTTAGTCATTTAAATGAC
AGCAATTATGAAGAAATTTTTGCTGCAGAACTTATAAGTGACATGATAAATGTCAATACA
AAGATACGACCAGTTGCTGATGCAATTATAAGACATCCATTATTTTGGAGTGAAGGAAAA
ATGTTGGATTTTCTTCATAGTGTTTCTGATAAAGTTGAAAAATTAAATACAACTGAAGAT
CCACTTTGGTCACTTGAAAGAAATGCAAAAATGATTGTTAGAGAAGATTGGAAGAAAATT
ATTGATGAAGAAATTTTAAATGATTTAGGAAAGCAAAGAACTTATCATGGAATTAGTGTG
AGAGATCTTTTAAGAGCTATAAGAAATAAACGCAATCATTACATGGAACTGACAGAAGAA
GTTAAAAATATTCTCGGATCAATTCCAGTTGATTACTTAAATTATTGGCTATCAAAATTT
CCTCATTTAGCTCCACATGCTTATCATGTAATGTCATCAAATCAAAAAGATTCAACATTT
TCAAGATTTTATGTTCGTTATCATCTTTTTACAAAACCTAGTTACATATCAGACACAAAT
CTTGACAATCATGAATTGGTTGAATTACAAGCAAAATGTAGAGCTGAAAATGCAAATTCT
AAATTTAATAACAATCAAAACTTTCAAAACCATGGAAGTCCAAATGGAAGAAAATTCTAC
AATAACAATAATTGGAATAAAAATAAAAATAGCAAACGAGGATTTTATAATTTTAAAAAT
AATGAAACGTGA
>g16433.t1 Gene=g16433 Length=903
MKINFKFLIFIFIFQWINLGKTDDDLRKIARSDEPLMVLVTLSGSMIGICPQTATQRWIK
QHDPIVKTNGNENLNFSTILLPDPVTGKLYNMKTSDKDKYELSQMDFTIPDLVLKTPFIS
KDKILYTGRKLDSWFMINSLTGECKTVMDFDDNKDKKTTDLIPFPTIHDVYIGKTQYDVC
MTNMYPDNKTREIWNVSMYQYNAFEMNKEIYDKYEFNHIVSSSSGKILTLFKTTGKIFWH
KTDLNSPVVALFMMGRNGFLNIPFTTVDDSVFEKLLEPVDYDKLKDIQLLETIFIGENKS
AGSNSFYALPTLSDNTTFIIRDKVINFLKDPSSIIGHYLLPSSNSAETNSKLITDESQSN
KSTELKNENKNEKIAVILALVVILISLVITFGIIVIKRKLKNETKKNNVSESDEQIVVDS
KIDEDGRTVISKIKFHRNDLIGRGSEGTSVFKGTFEGREVAVKRLLTSNFSLAEREVKIL
QKSDANENIIRYFCTEFDREFCYIAIELCACSLYDFTVNEDFQYLKDLIKPKEVLYQATK
GLSYLHQLGIIHRDIKPQNILISKPDGRKNIKVMISDFGLCKKFSKGEISVTNRIGIAGT
EGWIAPEILMNEKSSKIADIFSLGCVFYFTLTNGQHPFGDSLTRTSNILANNFNLSHLND
SNYEEIFAAELISDMINVNTKIRPVADAIIRHPLFWSEGKMLDFLHSVSDKVEKLNTTED
PLWSLERNAKMIVREDWKKIIDEEILNDLGKQRTYHGISVRDLLRAIRNKRNHYMELTEE
VKNILGSIPVDYLNYWLSKFPHLAPHAYHVMSSNQKDSTFSRFYVRYHLFTKPSYISDTN
LDNHELVELQAKCRAENANSKFNNNQNFQNHGSPNGRKFYNNNNWNKNKNSKRGFYNFKN
NET
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g16433.t1 | Coils | Coil | Coil | 760 | 780 | - |
| 12 | g16433.t1 | Coils | Coil | Coil | 900 | 903 | - |
| 9 | g16433.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 427 | 507 | 3.2E-31 |
| 10 | g16433.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 508 | 697 | 1.2E-46 |
| 11 | g16433.t1 | Gene3D | G3DSA:1.20.1440.180 | - | 699 | 839 | 1.6E-42 |
| 3 | g16433.t1 | PANTHER | PTHR13954:SF15 | SERINE/THREONINE-PROTEIN KINASE/ENDORIBONUCLEASE IRE2 | 30 | 280 | 3.1E-185 |
| 5 | g16433.t1 | PANTHER | PTHR13954 | IRE1-RELATED | 30 | 280 | 3.1E-185 |
| 4 | g16433.t1 | PANTHER | PTHR13954:SF15 | SERINE/THREONINE-PROTEIN KINASE/ENDORIBONUCLEASE IRE2 | 402 | 828 | 3.1E-185 |
| 6 | g16433.t1 | PANTHER | PTHR13954 | IRE1-RELATED | 402 | 828 | 3.1E-185 |
| 2 | g16433.t1 | Pfam | PF00069 | Protein kinase domain | 435 | 695 | 1.7E-42 |
| 1 | g16433.t1 | Pfam | PF06479 | Ribonuclease 2-5A | 702 | 824 | 4.8E-36 |
| 16 | g16433.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 22 | - |
| 17 | g16433.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 18 | g16433.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 17 | - |
| 20 | g16433.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 22 | - |
| 15 | g16433.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 23 | 373 | - |
| 19 | g16433.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 374 | 396 | - |
| 14 | g16433.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 397 | 903 | - |
| 22 | g16433.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 550 | 562 | - |
| 26 | g16433.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 435 | 695 | 36.047 |
| 25 | g16433.t1 | ProSiteProfiles | PS51392 | KEN domain profile. | 698 | 826 | 29.968 |
| 24 | g16433.t1 | SMART | SM00220 | serkin_6 | 435 | 695 | 1.0E-45 |
| 23 | g16433.t1 | SMART | SM00580 | PGNneu | 758 | 812 | 1.1E-11 |
| 7 | g16433.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 435 | 700 | 1.08E-55 |
| 8 | g16433.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
| 21 | g16433.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 374 | 396 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006397 | mRNA processing | BP |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0004540 | ribonuclease activity | MF |
KEGG
Orthology
- This transcript has been annotated with K08852 (serine/threonine-protein kinase/endoribonuclease IRE1 [EC:2.7.11.1 3.1.26.-])
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
