Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Serine/threonine-protein kinase/endoribonuclease ire-1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16433 g16433.t15 isoform g16433.t15 9424270 9426659
chr_4 g16433 g16433.t15 exon g16433.t15.exon1 9424270 9424673
chr_4 g16433 g16433.t15 cds g16433.t15.CDS1 9424271 9424673
chr_4 g16433 g16433.t15 exon g16433.t15.exon2 9424841 9425066
chr_4 g16433 g16433.t15 cds g16433.t15.CDS2 9424841 9425066
chr_4 g16433 g16433.t15 exon g16433.t15.exon3 9425804 9425925
chr_4 g16433 g16433.t15 cds g16433.t15.CDS3 9425804 9425925
chr_4 g16433 g16433.t15 exon g16433.t15.exon4 9426017 9426092
chr_4 g16433 g16433.t15 cds g16433.t15.CDS4 9426017 9426092
chr_4 g16433 g16433.t15 exon g16433.t15.exon5 9426157 9426659
chr_4 g16433 g16433.t15 cds g16433.t15.CDS5 9426157 9426493
chr_4 g16433 g16433.t15 TSS g16433.t15 9426727 9426727
chr_4 g16433 g16433.t15 TTS g16433.t15 NA NA

Sequences

>g16433.t15 Gene=g16433 Length=1331
ATGAAAATCAATTTCAAGTTTTTAATTTTTATTTTTATCTTTCAATGGATAAATTTGGGT
AAAACTGATGATGATTTGGTAAGAAAATTCCAAAATTTATCTTTGGAAAAAAAATAAATT
TAATAAAAAATTTTAGAGAAAAATCGCAAGATCAGATGAACCATTAATGGTTCTTGTGAC
ACTCTCAGGATCAATGATTGGAATCTGTCCACAAACAGCAACACAGAGATGGATAAAACA
ACATGATCCAATTGTCAAAACAAATGGAAATGAAAATTTAAATTTTTCAACAATTTTATT
ACCTGATCCAGTGACTGGAAAATTGTATAACATGAAAACTAGCGATAAAGACAAATATGA
ACTTTCACAAATGGATTTTACAATTCCTGACTTGGTACTTAAAACTCCTTTTATTTCTAA
GGATAAAATTTTATACACTGGAAGAAAATTGGATTCTTGGTTTATGATTAACTCACTAAC
TGGTGAATGCAAGACTGTTATGGATTTTGATGATAACAAAGATAAAAAGACAACAGATTT
AATACCATTTCCAACAATTCATGATGTCTACATAGGAAAAACACAATATGATGTTTGTAT
GACAAATATGTACCCTGATAACAAAACTAGAGAAATATGGAATGTTTCCATGTATCAGTA
CAATGCATTTGAAATGAATAAAGAAATTTATGACAAATATGAATTCAATCACATTGTCAG
CAGTTCAAGTGGGAAAATTCTAACACTTTTTAAAACAACAGGAAAAATTTTCTGGCATAA
AACAGATTTGAATTCACCAGTCGTTGCCTTATTTATGATGGGTAGAAACGGATTTTTAAA
CATTCCATTTACTACAGTTGATGATTCAGTATTTGAAAAGCTTTTGGAGCCTGTTGATTA
TGATAAATTAAAAGATATTCAATTACTTGAAACAATTTTTATCGGTGAAAATAAAAGTGC
AGGCTCAAATTCATTTTATGCTTTACCAACATTATCAGACAATACAACATTTATAATTCG
TGACAAAGTGATAAATTTTTTAAAAGATCCTTCATCAATTATTGGTCATTATTTATTACC
ATCATCAAATTCAGCTGAAACAAATTCAAAATTAATCACTGATGAATCACAAAGCAATAA
ATCTACAGAACTAAAAAATGAAAACAAAAATGAAAAAATCGCAGTGATTTTAGCATTGGT
TGTAATTTTAATTTCCTTGGTGATAACTTTTGGCATAATTGTGATAAAAAGAAAATTAAA
AAATGAAACAAAAAAGAATAATGTCAGTGAAAGCGATGAACAAATCGTCGTGGATTCAAA
AATAGATGAAG

>g16433.t15 Gene=g16433 Length=388
MVLVTLSGSMIGICPQTATQRWIKQHDPIVKTNGNENLNFSTILLPDPVTGKLYNMKTSD
KDKYELSQMDFTIPDLVLKTPFISKDKILYTGRKLDSWFMINSLTGECKTVMDFDDNKDK
KTTDLIPFPTIHDVYIGKTQYDVCMTNMYPDNKTREIWNVSMYQYNAFEMNKEIYDKYEF
NHIVSSSSGKILTLFKTTGKIFWHKTDLNSPVVALFMMGRNGFLNIPFTTVDDSVFEKLL
EPVDYDKLKDIQLLETIFIGENKSAGSNSFYALPTLSDNTTFIIRDKVINFLKDPSSIIG
HYLLPSSNSAETNSKLITDESQSNKSTELKNENKNEKIAVILALVVILISLVITFGIIVI
KRKLKNETKKNNVSESDEQIVVDSKIDE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
1 g16433.t15 PANTHER PTHR13954:SF15 SERINE/THREONINE-PROTEIN KINASE/ENDORIBONUCLEASE IRE2 3 245 3.1E-33
2 g16433.t15 PANTHER PTHR13954 IRE1-RELATED 3 245 3.1E-33
5 g16433.t15 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 337 -
6 g16433.t15 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 338 360 -
4 g16433.t15 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 361 388 -
3 g16433.t15 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 338 360 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values