General information for g16541.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Insulin-like receptor.
Parent gene
- The parent gene g16541 is located on chr_4: 9864531..9869096 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16541 | g16541.t1 | TSS | g16541.t1 | 9860594 | 9860594 |
| chr_4 | g16541 | g16541.t1 | isoform | g16541.t1 | 9860780 | 9869096 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon1 | 9860780 | 9861092 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS1 | 9860780 | 9861092 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon2 | 9864113 | 9864531 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS2 | 9864113 | 9864531 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon3 | 9864729 | 9865037 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS3 | 9864729 | 9865037 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon4 | 9865116 | 9866216 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS4 | 9865116 | 9866216 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon5 | 9866376 | 9866493 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS5 | 9866376 | 9866493 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon6 | 9866776 | 9867852 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS6 | 9866776 | 9867852 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon7 | 9867952 | 9868429 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS7 | 9867952 | 9868429 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon8 | 9868487 | 9868628 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS8 | 9868487 | 9868628 |
| chr_4 | g16541 | g16541.t1 | exon | g16541.t1.exon9 | 9868698 | 9869096 |
| chr_4 | g16541 | g16541.t1 | cds | g16541.t1.CDS9 | 9868698 | 9869096 |
| chr_4 | g16541 | g16541.t1 | TTS | g16541.t1 | NA | NA |
Sequences
>g16541.t1 Gene=g16541 Length=4356
ATGGAAAAAGTCATCAAAAATACAACATACACGAATCATTTACCGCGTGGATATTTTAAA
AAACGAAAACCACCTGATGAAATTATATCAAGTGACGAAACAAGTTCATCATCAATGCAT
ATAAATAGTTACAGTAGTCTAAGTTGTAGATTTAGTGATAATCGATATGTAAATAAATTT
TTTCAATTATGCACATTTTTAATATTAACTACTGTTCTTGGTGTAAACGCAAATGACGAA
TTACGACTAAGACGAGCTATTGAGAATTTTTCACAAACAACGCCAACACCATTAACATTG
CCTAATGATGGAATATGTAAAACGATTGATGTCCGTTTTGATTTAGAAAAGATTCACAGT
TTAGCAGGTTGTCGAGTAGTAGAAGGATCAGTTACAATTCTTGTCTTAGATGAAATCACC
AGTAAAGAAGTTGAAAACATAACTTTTCCAGAACTAGTTGAAGTTACAGGCTATGTTCTG
CTTTATCGTATAAGCGGCTTTACAAGTCTCAACCAACTTTTTCCAAATCTCGCTGTTATT
CGTGGAAGAGATCTGTTCAAAGATTATTCATTTGTTGTTTATGAAATGATTCAATTGGAA
GATATTGGATTAAGAAATTTAATGGCTATTGAAAGAGGAAATGTGAGAATTGAAAAAAAT
GAACTGTTGTGCTTTTCAAAGTCAATAAATTGGAAAAATATTATTCTACAAAATTTTGAT
CCTTATATTGAGAAAAACAAACTTCCAGCTCACTGTCCAAACTGTCCTAACGATAAATGC
AGCAAATCAATAGATGGAAAAAATACATACTGTTGGAATATCGAACACTGTCAAAGAACC
TGCTCAAAATGTCAATCTTCAAATACAAAGAATGAAATGACAATTTACTGCTCACCAAAT
ACTCAAACATGTTGCGATCCTAGTTGCATTGGTGGATGTGAAGATGATAAACCAAAAAAT
TGCTTTGCATGTCGAAAATTTGCTCTAGGAAATTATCCAAATATGCAATGTTTAGACAAA
TGTCCTAATAATACTTATGCTTACCTCGATCGACGATGCTTAACAAAAGATCAATGTTAT
GACCTCAATACAGCAACTGCAACGGACAGCAGTGTCAAAAATCCTGATGCACTGTTTATT
CCTTTTGAAAATGAATGTCGAAAAGATTGTCCACAAAATTATACAAAAGTCAACACTGGA
AGAAATATGACTTGTGAAAAATGTAACGGTCCATGTCTAAAGGAGTGCACATTTATAAAT
ATTGTTGATTCAATTGAAGCAGCTCAAGCATTAAAAGGATGCAACATCATTGAAGGACCT
TTAGAAATTCAAATTCGAGGAATAACAAAAAATCCTGAATCAGGCAAAAATATTGTGAAA
GAACTTGAAGCTTCACTTTCAGGCATTGAAGAAATTAAAGGTTATTTAAAAATTGCTCGA
TCGCATCCAATTCTTTCAATGTCTTTTCTCAAAAAACTTCGTAAAATTCATGGAAATAGT
CTTGAAGGTGGACGTAATGCACTTGTTGTTTGGGAAAATCAAAACTTACAAGAACTTTGG
GATGAATCACATCGAATCGAAATTGTTCAAGGAAAACTTTTCTTTCATTTTAATCCAAAA
CTTTGTTTTTATAAAATTGAACAATTGGCAAATAAAAGTGCAGATGGAAGGAAAAATCCA
ACAGAAATGATAGAAAATTATGATACAGCAAAAATTTCAAATGGTGACAAAACTCCATGC
AATGTCACGCCTTTGAATGTCACAGTTGAAAAAATTCTTCCTAAAGCTGCATTGCTTAGT
TGGAATCCATTGCAACTTGGTGATGAAAGATCTTTGCTTAATTATGTTGTTTACTACATT
GCTGCTCCTAATAAAAATGTTACATTATGGGATGGACGTGATGCATGTGGAAATGATGGT
TGGAGTGTTGAAGATGTCAATGATTTTAGTCATGTTCAGAAAATTACACAGCCATTGACA
AAATTGGAACCATATACACGATATGCTTATTATGTTAAAGCTTACACGTTAGCAACAGAA
AATACTGGAGCACAAAGTGAAATTAGTTATTTTACCACAATGCCTGACATACCAGAAGTT
GTGAAAAGACTTAAATTGACTGCTACTGGACCTGATAAAATTGAAGTAACTTGGGAAACA
CTCAAGAAAGTCAATGGAGAATTAGATCACTATATTTTAAGAGCAACTGCTATTCCTCAG
GATGATAAATTATTGGTACAAAGAGATTATTGCAAAGAACCAATTGACAATACACCAACA
CCATCAACACCATCGAAAGATACATCAATAATAAAAGATGGACCTAAAAATCCAGTAATA
GCACCAGATGGAACTTGTGATTGTCAGAAATGTTCATCAGTTTGTGATAGTAAAACACCA
CTATTTGATGGAGATGATGATCGACAATCAGCAAATGATTTTGAAGATCAAATTCAAGAT
TTTGTTTATAGAAAAAATGATGAAAAACCAAGTGCACAAAAAGAAACAAGAGCAAAACGA
CAAGTTCCAACTGAAATTGAATTTCCATCAAATCCACCAAATGATGGCTCAAGTTCAAAT
ACATTTGATCGAGAATATTTGAAAAATCAAAGTGATTTTTATGTTGAAGATATTGGACCT
GAAAATACAAGCTATGTCTTTACTGGACTTAATCATTACACATTTTATAATATTCGAATT
CGAGCATGTAGAAAACGTGAAAAAGGTGAAGCAGCAGATGCTGAAATTTGTGGACCAGAA
ACAAAAGTTTCTGAAACTACTTTCAAAGAACCAGCAAATGATGATATTTTAACTTTCAAA
GTGGAACCAATTGTGAATAATTCATTGACAAATATTCGAGTCACTTGGGAACCACCAAAT
TCACCCAATGGATTATTACTTGCATTTACAATAAGACATAAAAGAGTTGACATTGATCAT
ACTCAGCCTGTTTTGCATTGTATATCAACAAGTATGCTCAATGGCACTAATCAATATGTT
TTACAGAAATTGGCACCTGGTAATTATAGCATTGATATGATGGCAACATCAATGGCTGGT
AATGGAAATTATACAAAAGCACAATATGTATTGATAAAAGAACAAGGTAATTGGAATAAC
TTATGGATAGTTTTCATTGTATTATTGGTGCTATTTGTTCTTGCCTCAATTGTTTTGTAT
ATAACTTTCAAACGATTATACCTCACAAATATCTCGAGTATGAAATTAATTGCAAATGTT
AATCCTGATTATGCTGGTGTAACTTATCGACAAGATGAATGGGAAATTCCTAGAGAAAAA
ATTATTCAATTGCAAGAACTTGGTTGTGGAAGTTTTGGTATGGTCTATGAAGGACTCATT
AAGGATTTCCGTCAACCAGGTGATGACATTCGTTGTGCAATAAAAACAGTTAATGAAAGT
GCAACTGACAAAGAACGTATAAGTTTTCTAAATGAAGCATCTGTAATGAAACAATTTGAC
ACTCATCATGTAGTTCGTCTTCTTGGTGTTGTGTCACAAGGACAACCAACTCTTGTAGTT
ATGGAATTAATGGCAAATGGTGATTTGAAAAGTTATTTAAGATCACATAGACCTGAATAT
GATACAATTGGAACTGAACCACCACCACAGCCACCAACATTGAGAAGAATTCTTCAAATG
GCAATTGAAATTGCTGATGGCATGGCATACTTGGCAGCAAAGAAATTTGTACATCGCGAT
CTTGCTGCTAGAAATTGCATGGTTGCTGAAGATATGACAGTTAAAATTGGTGACTTTGGT
ATGACTCGAGATATTTATGAAACTGATTATTACAGGAAAGGCACAAAAGGACTTTTGCCA
GTTCGTTGGATGAGTCCTGAATCTTTAAAAGATGGTGTTTTTACATCAAGTAGTGATGTC
TTCAGTTATGGTGTTGTTGTTTGGGAAATGTCAACACTTGCACAACAACCTTATGCTGGT
CTTTCAAATGATCAAGTTTTACGCTATGTTATCGAAGGAGGCGTAATGGAAAGACCAGAA
AATTGTCCTGACAAACTATACGATCTGATGAGAAAATGTTGGCAACATCGACCTTCAGCA
CGTCCATCATTTATGGAAATCATCTCAATGCTTTTAGACAGTGTCAGTTCAGACTTTGAA
AAGGTGAGTTTCTTCCATTCACCAGCTGGTGTTGAAGCATGGCATCAAATACAACAAAAT
GAAGTTGCTGTGCATGTCACTGATGATGTAACGACACCATTGAGACAAGATGAAGATGAA
GACGAAGATGAAGATGATATTCCGAGTTATGATAGAAATCATTTTAATTTGGAAGTTGTA
AGTGGTCTTAAACATCGACGTGATGATGACCTGTAA
>g16541.t1 Gene=g16541 Length=1451
MEKVIKNTTYTNHLPRGYFKKRKPPDEIISSDETSSSSMHINSYSSLSCRFSDNRYVNKF
FQLCTFLILTTVLGVNANDELRLRRAIENFSQTTPTPLTLPNDGICKTIDVRFDLEKIHS
LAGCRVVEGSVTILVLDEITSKEVENITFPELVEVTGYVLLYRISGFTSLNQLFPNLAVI
RGRDLFKDYSFVVYEMIQLEDIGLRNLMAIERGNVRIEKNELLCFSKSINWKNIILQNFD
PYIEKNKLPAHCPNCPNDKCSKSIDGKNTYCWNIEHCQRTCSKCQSSNTKNEMTIYCSPN
TQTCCDPSCIGGCEDDKPKNCFACRKFALGNYPNMQCLDKCPNNTYAYLDRRCLTKDQCY
DLNTATATDSSVKNPDALFIPFENECRKDCPQNYTKVNTGRNMTCEKCNGPCLKECTFIN
IVDSIEAAQALKGCNIIEGPLEIQIRGITKNPESGKNIVKELEASLSGIEEIKGYLKIAR
SHPILSMSFLKKLRKIHGNSLEGGRNALVVWENQNLQELWDESHRIEIVQGKLFFHFNPK
LCFYKIEQLANKSADGRKNPTEMIENYDTAKISNGDKTPCNVTPLNVTVEKILPKAALLS
WNPLQLGDERSLLNYVVYYIAAPNKNVTLWDGRDACGNDGWSVEDVNDFSHVQKITQPLT
KLEPYTRYAYYVKAYTLATENTGAQSEISYFTTMPDIPEVVKRLKLTATGPDKIEVTWET
LKKVNGELDHYILRATAIPQDDKLLVQRDYCKEPIDNTPTPSTPSKDTSIIKDGPKNPVI
APDGTCDCQKCSSVCDSKTPLFDGDDDRQSANDFEDQIQDFVYRKNDEKPSAQKETRAKR
QVPTEIEFPSNPPNDGSSSNTFDREYLKNQSDFYVEDIGPENTSYVFTGLNHYTFYNIRI
RACRKREKGEAADAEICGPETKVSETTFKEPANDDILTFKVEPIVNNSLTNIRVTWEPPN
SPNGLLLAFTIRHKRVDIDHTQPVLHCISTSMLNGTNQYVLQKLAPGNYSIDMMATSMAG
NGNYTKAQYVLIKEQGNWNNLWIVFIVLLVLFVLASIVLYITFKRLYLTNISSMKLIANV
NPDYAGVTYRQDEWEIPREKIIQLQELGCGSFGMVYEGLIKDFRQPGDDIRCAIKTVNES
ATDKERISFLNEASVMKQFDTHHVVRLLGVVSQGQPTLVVMELMANGDLKSYLRSHRPEY
DTIGTEPPPQPPTLRRILQMAIEIADGMAYLAAKKFVHRDLAARNCMVAEDMTVKIGDFG
MTRDIYETDYYRKGTKGLLPVRWMSPESLKDGVFTSSSDVFSYGVVVWEMSTLAQQPYAG
LSNDQVLRYVIEGGVMERPENCPDKLYDLMRKCWQHRPSARPSFMEIISMLLDSVSSDFE
KVSFFHSPAGVEAWHQIQQNEVAVHVTDDVTTPLRQDEDEDEDEDDIPSYDRNHFNLEVV
SGLKHRRDDDL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 33 | g16541.t1 | CDD | cd00064 | FU | 304 | 354 | 4.58325E-7 |
| 36 | g16541.t1 | CDD | cd00063 | FN3 | 584 | 693 | 1.87554E-7 |
| 35 | g16541.t1 | CDD | cd00063 | FN3 | 944 | 1030 | 1.60532E-6 |
| 34 | g16541.t1 | CDD | cd05032 | PTKc_InsR_like | 1094 | 1373 | 0.0 |
| 27 | g16541.t1 | Gene3D | G3DSA:3.80.20.20 | - | 100 | 336 | 1.4E-64 |
| 29 | g16541.t1 | Gene3D | G3DSA:2.10.220.10 | Hormone Receptor | 337 | 411 | 2.2E-14 |
| 28 | g16541.t1 | Gene3D | G3DSA:3.80.20.20 | - | 412 | 590 | 1.5E-51 |
| 24 | g16541.t1 | Gene3D | G3DSA:2.60.40.10 | Immunoglobulins | 591 | 693 | 2.2E-33 |
| 22 | g16541.t1 | Gene3D | G3DSA:2.60.40.10 | Immunoglobulins | 702 | 783 | 8.0E-5 |
| 21 | g16541.t1 | Gene3D | G3DSA:2.60.40.10 | Immunoglobulins | 819 | 927 | 1.8E-7 |
| 23 | g16541.t1 | Gene3D | G3DSA:2.60.40.10 | Immunoglobulins | 934 | 1038 | 9.9E-16 |
| 25 | g16541.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 1077 | 1182 | 2.3E-32 |
| 26 | g16541.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1183 | 1393 | 1.8E-64 |
| 48 | g16541.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 754 | 778 | - |
| 47 | g16541.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 756 | 770 | - |
| 49 | g16541.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 822 | 842 | - |
| 50 | g16541.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 822 | 860 | - |
| 51 | g16541.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1411 | 1433 | - |
| 5 | g16541.t1 | PANTHER | PTHR24416:SF106 | INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR | 102 | 227 | 1.4E-277 |
| 7 | g16541.t1 | PANTHER | PTHR24416 | TYROSINE-PROTEIN KINASE RECEPTOR | 102 | 227 | 1.4E-277 |
| 6 | g16541.t1 | PANTHER | PTHR24416:SF106 | INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR | 257 | 1387 | 1.4E-277 |
| 8 | g16541.t1 | PANTHER | PTHR24416 | TYROSINE-PROTEIN KINASE RECEPTOR | 257 | 1387 | 1.4E-277 |
| 10 | g16541.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 1181 | 1194 | 9.4E-32 |
| 11 | g16541.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 1230 | 1248 | 9.4E-32 |
| 12 | g16541.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 1279 | 1289 | 9.4E-32 |
| 9 | g16541.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 1298 | 1320 | 9.4E-32 |
| 13 | g16541.t1 | PRINTS | PR00109 | Tyrosine kinase catalytic domain signature | 1342 | 1364 | 9.4E-32 |
| 3 | g16541.t1 | Pfam | PF01030 | Receptor L domain | 123 | 234 | 2.7E-22 |
| 1 | g16541.t1 | Pfam | PF00757 | Furin-like cysteine rich region | 252 | 417 | 1.6E-23 |
| 2 | g16541.t1 | Pfam | PF01030 | Receptor L domain | 433 | 549 | 6.9E-24 |
| 4 | g16541.t1 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 1104 | 1371 | 2.9E-94 |
| 31 | g16541.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 1040 | - |
| 32 | g16541.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1041 | 1063 | - |
| 30 | g16541.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1064 | 1451 | - |
| 46 | g16541.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 1107 | 1135 | - |
| 44 | g16541.t1 | ProSitePatterns | PS00109 | Tyrosine protein kinases specific active-site signature. | 1236 | 1248 | - |
| 45 | g16541.t1 | ProSitePatterns | PS00239 | Receptor tyrosine kinase class II signature. | 1264 | 1272 | - |
| 52 | g16541.t1 | ProSiteProfiles | PS50853 | Fibronectin type-III domain profile. | 583 | 696 | 13.365 |
| 54 | g16541.t1 | ProSiteProfiles | PS50853 | Fibronectin type-III domain profile. | 829 | 931 | 9.586 |
| 53 | g16541.t1 | ProSiteProfiles | PS50853 | Fibronectin type-III domain profile. | 937 | 1035 | 10.075 |
| 55 | g16541.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1101 | 1385 | 35.156 |
| 43 | g16541.t1 | SMART | SM00261 | furin_3 | 301 | 347 | 1.0E-7 |
| 39 | g16541.t1 | SMART | SM00060 | FN3_2 | 581 | 681 | 0.0011 |
| 41 | g16541.t1 | SMART | SM00060 | FN3_2 | 698 | 909 | 2.4E-5 |
| 40 | g16541.t1 | SMART | SM00060 | FN3_2 | 930 | 1022 | 4.0 |
| 42 | g16541.t1 | SMART | SM00219 | tyrkin_6 | 1101 | 1371 | 1.8E-135 |
| 19 | g16541.t1 | SUPERFAMILY | SSF52058 | L domain-like | 103 | 273 | 3.12E-35 |
| 17 | g16541.t1 | SUPERFAMILY | SSF57184 | Growth factor receptor domain | 304 | 418 | 1.33E-8 |
| 18 | g16541.t1 | SUPERFAMILY | SSF52058 | L domain-like | 410 | 581 | 5.67E-35 |
| 14 | g16541.t1 | SUPERFAMILY | SSF49265 | Fibronectin type III | 580 | 695 | 4.59E-27 |
| 15 | g16541.t1 | SUPERFAMILY | SSF49265 | Fibronectin type III | 698 | 910 | 9.27E-12 |
| 16 | g16541.t1 | SUPERFAMILY | SSF49265 | Fibronectin type III | 867 | 1031 | 4.78E-16 |
| 20 | g16541.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 1077 | 1371 | 9.02E-79 |
| 38 | g16541.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 60 | 77 | - |
| 37 | g16541.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1041 | 1063 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005524 | ATP binding | MF |
| GO:0006468 | protein phosphorylation | BP |
| GO:0004713 | protein tyrosine kinase activity | MF |
| GO:0007169 | transmembrane receptor protein tyrosine kinase signaling pathway | BP |
| GO:0005515 | protein binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0004714 | transmembrane receptor protein tyrosine kinase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K05087 (insulin-like growth factor 1 receptor)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
