General information for g16542.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Thiamin pyrophosphokinase 1.
Parent gene
- The parent gene g16542 is located on chr_4: 9873958..9875372 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16542 | g16542.t1 | TSS | g16542.t1 | 9873735 | 9873735 |
| chr_4 | g16542 | g16542.t1 | isoform | g16542.t1 | 9873768 | 9874586 |
| chr_4 | g16542 | g16542.t1 | exon | g16542.t1.exon1 | 9873768 | 9873900 |
| chr_4 | g16542 | g16542.t1 | cds | g16542.t1.CDS1 | 9873768 | 9873900 |
| chr_4 | g16542 | g16542.t1 | exon | g16542.t1.exon2 | 9873958 | 9874586 |
| chr_4 | g16542 | g16542.t1 | cds | g16542.t1.CDS2 | 9873958 | 9874586 |
| chr_4 | g16542 | g16542.t1 | TTS | g16542.t1 | NA | NA |
Sequences
>g16542.t1 Gene=g16542 Length=762
ATGAACAAATTTTTTCCAAGTAAAATCATCACCAAGAAAGAGGTTTTAGAGTCAAATTTG
AATTATGCCTTGATCATTCTTAATCGACCAATTCAAATAAAAAGAGAACTCATCGAAACA
CTTTGGAATAATTCATTAATAAACATAACAGTCGATGGTGGAACAAATCGTTGGCTTAAT
TTCCTTAAAAATAACAATCTCGAAAATAAGCTGAAACATCCAACATTAATCACTGGTGAT
CTCGATTCATGTCATGCTGAAAGTTTAAAATTTTTTACTCAAACCAAAGTCATTCAAACT
GTCGATCAGGATGAGACTGATTTTACAAAATGTCTTAGAGTTCTTGAACCTTTCATAAAT
GAACTTAAACTTGATCATACTGTAGTGATTTGTGAAAATTCTGGTCGACTTGATCACATT
ATTGCAAATATTAATACTTTGTATAAAAATCAACTTAAATCGAATGAAATCTCAAGGCCT
GTTTTTATGTTATCATCGACTAGCATTTCATTTTTATTACCAGAAGGTAAAAGTGAAATT
CACATTCCTGAATGCGCAAAGAAATTATGGTGTGCACTTATGCCAATTGCTGGACCGACA
ACTGTTAGCACAGAAGGATTAAAATGGAATATGAAAAATTTTGAAATGAAATTTGGTGGA
TTAGTCAGTACAAGTAATAAATATGATGGAATATCAGATTTTGTAAAAGTTGAATGTAAT
TTGCCAATCCTTTGGTCAATGGGAATTTGTGATGAAATTTAA
>g16542.t1 Gene=g16542 Length=253
MNKFFPSKIITKKEVLESNLNYALIILNRPIQIKRELIETLWNNSLINITVDGGTNRWLN
FLKNNNLENKLKHPTLITGDLDSCHAESLKFFTQTKVIQTVDQDETDFTKCLRVLEPFIN
ELKLDHTVVICENSGRLDHIIANINTLYKNQLKSNEISRPVFMLSSTSISFLLPEGKSEI
HIPECAKKLWCALMPIAGPTTVSTEGLKWNMKNFEMKFGGLVSTSNKYDGISDFVKVECN
LPILWSMGICDEI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g16542.t1 | CDD | cd07995 | TPK | 23 | 238 | 0 |
| 8 | g16542.t1 | Gene3D | G3DSA:3.40.50.10240 | Thiamin pyrophosphokinase | 22 | 166 | 0 |
| 9 | g16542.t1 | Gene3D | G3DSA:2.60.120.320 | - | 167 | 247 | 0 |
| 3 | g16542.t1 | PANTHER | PTHR13622:SF8 | THIAMIN PYROPHOSPHOKINASE 1 | 18 | 249 | 0 |
| 4 | g16542.t1 | PANTHER | PTHR13622 | THIAMIN PYROPHOSPHOKINASE | 18 | 249 | 0 |
| 2 | g16542.t1 | Pfam | PF04263 | Thiamin pyrophosphokinase, catalytic domain | 39 | 153 | 0 |
| 1 | g16542.t1 | Pfam | PF04265 | Thiamin pyrophosphokinase, vitamin B1 binding domain | 179 | 243 | 0 |
| 7 | g16542.t1 | SMART | SM00983 | TPK_B1_binding_a_2_a | 176 | 243 | 0 |
| 5 | g16542.t1 | SUPERFAMILY | SSF63999 | Thiamin pyrophosphokinase, catalytic domain | 21 | 150 | 0 |
| 6 | g16542.t1 | SUPERFAMILY | SSF63862 | Thiamin pyrophosphokinase, substrate-binding domain | 168 | 250 | 0 |
| 10 | g16542.t1 | TIGRFAM | TIGR01378 | thi_PPkinase: thiamine pyrophosphokinase | 24 | 247 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0030975 | thiamine binding | MF |
| GO:0005524 | ATP binding | MF |
| GO:0004788 | thiamine diphosphokinase activity | MF |
| GO:0009229 | thiamine diphosphate biosynthetic process | BP |
| GO:0006772 | thiamine metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K00949 (thiamine pyrophosphokinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
