General information for g16634.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Lipase 3.
Parent gene
- The parent gene g16634 is located on chr_4: 10350692..10351992 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16634 | g16634.t1 | isoform | g16634.t1 | 10350692 | 10351992 |
| chr_4 | g16634 | g16634.t1 | exon | g16634.t1.exon1 | 10350692 | 10350850 |
| chr_4 | g16634 | g16634.t1 | cds | g16634.t1.CDS1 | 10350692 | 10350850 |
| chr_4 | g16634 | g16634.t1 | exon | g16634.t1.exon2 | 10350988 | 10351992 |
| chr_4 | g16634 | g16634.t1 | cds | g16634.t1.CDS2 | 10350988 | 10351992 |
| chr_4 | g16634 | g16634.t1 | TSS | g16634.t1 | NA | NA |
| chr_4 | g16634 | g16634.t1 | TTS | g16634.t1 | NA | NA |
Sequences
>g16634.t1 Gene=g16634 Length=1164
ATGAAATTATTTATCATATTTTCAATCCTACTCATAATTACTCAATTAAAATCAGAAGAA
GATCTCACAATAAAACAAATCAATTCATTTGGGTTTAAAGGAGAAATACACAAAGTTGAA
ACAGCTGACAATTACTTACTGACCCTTCATCGAATTAAACCAAGAAAATTTACAAATTCA
TCACAAGTTGTTCTTTTGGTTCATGGAATTTGCTGCACATCAAGTGAATTTTTATCATCA
AAAAGAACTTCTTTGGCAATTTTTTTATTTGAAAATGGATTTGATGTTTTTCTTGGAAAT
GTTCGTGGAAATAAATACTCAAAAAGTCACAAAAATTTAAATCATGAAAACAAAGAATTT
TGGAATTTTTCACTTCATGAATTGGGAATTTTTGATCTCGCAGCTTTTATTGATTTTATA
TTGAAATTTACAAAAAATAAAAAATTATTTTACATTGGTCATTCACAAGGTGCAACAATT
CCTTTAATTTTGCTCAGTTCAAAGCCTGAATATAATGAAAAAATTTATCAACTACATTTA
TCAGCACCAGCACCATTCATGAGTCATTTTCCAAATGTTTTAATCAAAAAATATGGAAAA
CAAGCTGTGAAAACTTTTACAAAAAATAACTTGATAAATTTGACTCCAATTATTGAAGTT
TTACATCAAAATTTGTACAAACACTGCAGTTTAAATCAACCATTGTATTTTATAACATGC
ATGATTGTTGAATTTTTAATTCTTGGTGATAATTTCACAAAACTTGAAGCTGATCATGAA
ACTTTTGATAAACTTTTAACATTTTTATCACCAAACGTGAGCTCAACTCAAGTGTTGCAT
TTTTTTCAATTTATCGAAAGTGGAAAATTTCGACAGTTTGATTATGGAAGAAAAAAGAAT
TTGGAAAAATATGGAATGATTGAACCTCCTGAATATGATTTAAAAAAAGTTGTAACACCG
ACTTATATTTATTGTGCTGAACGAGATCGAGTTGTTGCTAGAAAAGATATCGAACACCTT
CGAGAAATTCTTCCCAATGTCATCACTTACAAGCTTCTAAAAAATTACAATCACATCGAC
TTTGATATCGGAATAAATGCAAGAAAAGATGTTCATAAAGACATTCTAAAAGCAATGAAA
ATGAATAGCAAAAGACAGAGTTGA
>g16634.t1 Gene=g16634 Length=387
MKLFIIFSILLIITQLKSEEDLTIKQINSFGFKGEIHKVETADNYLLTLHRIKPRKFTNS
SQVVLLVHGICCTSSEFLSSKRTSLAIFLFENGFDVFLGNVRGNKYSKSHKNLNHENKEF
WNFSLHELGIFDLAAFIDFILKFTKNKKLFYIGHSQGATIPLILLSSKPEYNEKIYQLHL
SAPAPFMSHFPNVLIKKYGKQAVKTFTKNNLINLTPIIEVLHQNLYKHCSLNQPLYFITC
MIVEFLILGDNFTKLEADHETFDKLLTFLSPNVSSTQVLHFFQFIESGKFRQFDYGRKKN
LEKYGMIEPPEYDLKKVVTPTYIYCAERDRVVARKDIEHLREILPNVITYKLLKNYNHID
FDIGINARKDVHKDILKAMKMNSKRQS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g16634.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 14 | 382 | 9.7E-106 |
| 2 | g16634.t1 | PANTHER | PTHR11005:SF127 | LIPASE-RELATED | 9 | 382 | 4.7E-100 |
| 3 | g16634.t1 | PANTHER | PTHR11005 | LYSOSOMAL ACID LIPASE-RELATED | 9 | 382 | 4.7E-100 |
| 12 | g16634.t1 | PIRSF | PIRSF000862 | Steryl_ester_lip | 3 | 384 | 5.2E-95 |
| 1 | g16634.t1 | Pfam | PF00561 | alpha/beta hydrolase fold | 63 | 361 | 3.6E-22 |
| 8 | g16634.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 9 | g16634.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 10 | g16634.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 13 | - |
| 11 | g16634.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 7 | g16634.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 387 | - |
| 4 | g16634.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 25 | 379 | 2.49E-51 |
| 5 | g16634.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016788 | hydrolase activity, acting on ester bonds | MF |
KEGG
Orthology
- This transcript has been annotated with K01052 (lysosomal acid lipase/cholesteryl ester hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed