Gene loci information

Transcript annotation

  • This transcript has been annotated as Esterase FE4.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_4 g16656 g16656.t1 TSS g16656.t1 10419429 10419429
chr_4 g16656 g16656.t1 isoform g16656.t1 10419471 10421519
chr_4 g16656 g16656.t1 exon g16656.t1.exon1 10419471 10420089
chr_4 g16656 g16656.t1 cds g16656.t1.CDS1 10419471 10420089
chr_4 g16656 g16656.t1 exon g16656.t1.exon2 10420201 10420623
chr_4 g16656 g16656.t1 cds g16656.t1.CDS2 10420201 10420623
chr_4 g16656 g16656.t1 exon g16656.t1.exon3 10420691 10421156
chr_4 g16656 g16656.t1 cds g16656.t1.CDS3 10420691 10421156
chr_4 g16656 g16656.t1 exon g16656.t1.exon4 10421216 10421415
chr_4 g16656 g16656.t1 cds g16656.t1.CDS4 10421216 10421415
chr_4 g16656 g16656.t1 exon g16656.t1.exon5 10421515 10421519
chr_4 g16656 g16656.t1 cds g16656.t1.CDS5 10421515 10421519
chr_4 g16656 g16656.t1 TTS g16656.t1 10421544 10421544

Sequences

>g16656.t1 Gene=g16656 Length=1713
ATGAAAATCATTAGCTTAATATTTTTCTCTATTTTCTTACAATTGTGCACAACTTACGAA
GAAATTGATGAATTATTAGTTGAAATAAATTCAGGAAAAATTTTAGGTAGAGCTTTAGTA
TCAAATTCAGGCAGAACAATTCGTGCTTTTACTGGTATTCCTTATGCAGCTCCACCAATT
GGAAATTTAAGATTCAAACAAGCTCAAAAAGTCACACCGTGGAATAATACATTCATAGCT
CAAAATAAACCAGCAAAATGTCTTCAATTTAATCCATTTATTCGATCATTTGTAATTGAA
GGTCAAGAAGATTGCCTAACTTTAAATGTTTTTGCACCACAAGAAATTGAAGGCAAAAAA
TTTCCAGTGATTGTTTGGTTTCATGGTGGCGGTTGGACTAGAGGTGATGCTTCTCCATAT
GGTCCAGAATATCTCTTAGATCACGACATTATTCTTGTCAGTGGTGAATATAGAGTTGGA
GCACTTGGTTTTTTATCAAGTGAAGACAAAAATGTTCCTGGCAATTTTGGGTTGAAAGAT
CAAGCTTTTATACTTAAATGGGTACAAGAAAATATTGAAAAATTTGGTGGTGATAAAAAC
TCAGTGACTATTTGGGGAGAATCTGCCGGAGGTGCATCAATCCATTATCACATGTATTCA
CCAATGTCACGTGGACTTTTTCATCGTGCAATTTCACATAGTGGAAGTTTATACAACTGC
TGGTCAGATCCAACTCGACCTGGAGTCGCTCGTAAAAATTTTTATCGTTTCGCTATCGAA
GCTGATTGTGCAAAATCAGAAAATGATAATACTGAGGAAGTTGCTGATTGCATGAGAAAA
ATTGAAGCTGAAAAATTGACTGAAATTTCAACAAGAAGTTATTTTTGGGATAATGATCCT
GTTGTACCTTTACAGCCTGTAATTGAATCTTTTGATATGGAAGAAGAACCATTTATTAAA
GAAAGAACTTTTGCTAATCATTCTGTCCATATTCCGTGGTTGATTGGGATGAATAGTGAA
GAAGGATTGCTAAAAGTTGGAGCAATGTTTAATGATAGAAATTTCCTAAACGAACTTCAC
CAAAAATGGAATGAAATCTTACCACATTCATTTTATTATGATCATCTTGATAACAGTCAA
CAAGTTGAGATTACAAAAATATTGAATGAATTTTATTTTGGTAACGAAACTTTATCAGAA
ACAACAATGAGTGATCCAAAAGGATTGATTGATATGTGGACAGATGGTTGGTTTATTGGA
ATTTTTGAAAGTCTAAAATATCGATTTGAAAATGGAGCACGAGACAACACTTTTATTTTT
CATTTTACTCATAAATCAACTGCAAGTTTTAGTATGGCAAATAATTTTCTTGGAACTTGC
CATGTTGATGATTTGATTCCACTTTTTCAACTGAGAAAATCTTTTTATTATAGTTCAGTC
CCGACAAGACATGATAAAGAACTTACTGAAAAAATGACAAAAATGTGGACAAATTTTGCT
ACATTTGGAAATCCAACACCAGAAAATATTTTGGGAATTCCGCAATGGCCAACGATAAAC
AACATTACTTCTTTTAAATATTTACAAATTGGAAATGAAAATGGAAAAACTGAAAGCCTT
TTTCAACTCAAAGAAAATTATTATATTGAAAGAGCTGAACTGTGGATGAATTTGAGAAAT
CATTTTAAATTAAATTCTTGGGAATCAGATTAA

>g16656.t1 Gene=g16656 Length=570
MKIISLIFFSIFLQLCTTYEEIDELLVEINSGKILGRALVSNSGRTIRAFTGIPYAAPPI
GNLRFKQAQKVTPWNNTFIAQNKPAKCLQFNPFIRSFVIEGQEDCLTLNVFAPQEIEGKK
FPVIVWFHGGGWTRGDASPYGPEYLLDHDIILVSGEYRVGALGFLSSEDKNVPGNFGLKD
QAFILKWVQENIEKFGGDKNSVTIWGESAGGASIHYHMYSPMSRGLFHRAISHSGSLYNC
WSDPTRPGVARKNFYRFAIEADCAKSENDNTEEVADCMRKIEAEKLTEISTRSYFWDNDP
VVPLQPVIESFDMEEEPFIKERTFANHSVHIPWLIGMNSEEGLLKVGAMFNDRNFLNELH
QKWNEILPHSFYYDHLDNSQQVEITKILNEFYFGNETLSETTMSDPKGLIDMWTDGWFIG
IFESLKYRFENGARDNTFIFHFTHKSTASFSMANNFLGTCHVDDLIPLFQLRKSFYYSSV
PTRHDKELTEKMTKMWTNFATFGNPTPENILGIPQWPTINNITSFKYLQIGNENGKTESL
FQLKENYYIERAELWMNLRNHFKLNSWESD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g16656.t1 Gene3D G3DSA:3.40.50.1820 - 3 564 4.1E-147
2 g16656.t1 PANTHER PTHR11559:SF395 CARBOXYLIC ESTER HYDROLASE 4 567 8.2E-144
3 g16656.t1 PANTHER PTHR11559 CARBOXYLESTERASE 4 567 8.2E-144
1 g16656.t1 Pfam PF00135 Carboxylesterase family 25 542 7.1E-128
10 g16656.t1 Phobius SIGNAL_PEPTIDE Signal peptide region 1 18 -
11 g16656.t1 Phobius SIGNAL_PEPTIDE_N_REGION N-terminal region of a signal peptide. 1 2 -
12 g16656.t1 Phobius SIGNAL_PEPTIDE_H_REGION Hydrophobic region of a signal peptide. 3 13 -
13 g16656.t1 Phobius SIGNAL_PEPTIDE_C_REGION C-terminal region of a signal peptide. 14 18 -
9 g16656.t1 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 19 570 -
5 g16656.t1 ProSitePatterns PS00941 Carboxylesterases type-B signature 2. 103 113 -
6 g16656.t1 ProSitePatterns PS00122 Carboxylesterases type-B serine active site. 195 210 -
4 g16656.t1 SUPERFAMILY SSF53474 alpha/beta-Hydrolases 23 526 6.31E-121
7 g16656.t1 SignalP_EUK SignalP-noTM SignalP-noTM 1 18 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values