General information for g16669.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Multiple epidermal growth factor-like domains protein 8.
Parent gene
- The parent gene g16669 is located on chr_4: 10455839..10456858 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_4 | g16669 | g16669.t3 | TSS | g16669.t3 | 10446253 | 10446253 |
| chr_4 | g16669 | g16669.t3 | isoform | g16669.t3 | 10446413 | 10448332 |
| chr_4 | g16669 | g16669.t3 | exon | g16669.t3.exon1 | 10446413 | 10446601 |
| chr_4 | g16669 | g16669.t3 | cds | g16669.t3.CDS1 | 10446413 | 10446601 |
| chr_4 | g16669 | g16669.t3 | exon | g16669.t3.exon2 | 10446655 | 10446750 |
| chr_4 | g16669 | g16669.t3 | cds | g16669.t3.CDS2 | 10446655 | 10446750 |
| chr_4 | g16669 | g16669.t3 | exon | g16669.t3.exon3 | 10446807 | 10448177 |
| chr_4 | g16669 | g16669.t3 | cds | g16669.t3.CDS3 | 10446807 | 10448177 |
| chr_4 | g16669 | g16669.t3 | exon | g16669.t3.exon4 | 10448236 | 10448332 |
| chr_4 | g16669 | g16669.t3 | cds | g16669.t3.CDS4 | 10448236 | 10448331 |
| chr_4 | g16669 | g16669.t3 | TTS | g16669.t3 | NA | NA |
Sequences
>g16669.t3 Gene=g16669 Length=1753
ATGAAAGCTAAAAAGCGTGGAAAATTTCAAAAAATAAACTTTTGTGCAATTTTTCATCTT
TCAATTTTAATTTTTGTTTCAAAATTTAATAAAATTGAAGCAGTTGGTTCATGTGATAGA
ACAAGACAAGTTTTTGAAGGTGTGAATTATGGAGAAATCACTGATGGTCTCAATTCTAAT
TACACTCAAGATTCTCATTGCGAATGGTTGATAAAAGCCGAAAATAGTTCACAATTTATT
ACATTAAAATTTCTATCACTTAAGACTGAATGTTCATATGACTATATTTTTATTTATGAT
GGAAATTCATATTCATCATCGCCATTACTCGGAAGTTTCAGTGGTCAAACTGATCCACAA
GATATTACAGCAACTTCCGGTTTTATGCTCATACTTTTATACAGTGATACAAATTACAAT
CTTGATGGCTTCAGAGCGACATTTAGTATCACAAATTGTCTCAAAAATTGCTCATCAAAT
GGACTTTGTGTAAATCATAAATGTCATTGCAATTCTGATTATTCTGGCAATGATTGTAGT
GTAAAAATTTGTAAAAATTGTGCAAATGAAAAATTATCATCGAATGAGTCACAATGGGTG
TGGATAACACAAAATTATAATAGTTTCACACCGAGAACAGCACACATTGCTATTTATGAT
GATGTCACAGATGCTGTTTACACATTTGGTGGTTATGACTTAAATAATATACTTGGAAAA
TTAGAAATTTATCAGTTTAATGATAGCAAATGGATTGATGAAGATGAAAAAGAAATTTCA
ATTGGAATAAAAAATGACAATGATGAATTTTGGTTTAGAGATGCTCTACTGGCTCATGTC
AATTCAAAAAGAAATAAAAGTAATTTAAATTTACATGAAAATGAAAAAATTCAAATCAGC
TCAGAAAAGGCACAAAATAACCAAAGAAATTTAAAAAGTATTAATTATGAATCTCAAAAA
TTTAAACCATTACCTCGTTATGGACATGCTGCATGTAAATTTAAAGATTCTTTCTTAATT
TATGGCGGTAAACTATATAATGGAAGTCTTTCAAATGAATTATGGATGTTCAACATATCA
ACAAAAATTTGGTCACTTAAAGCTAAAAATTCTTCTTTAATTCCACCAAAACTTACACGG
CATACATTGACTTTTGTAAATTTTAATAATTTTATTTATCTTTTTGGTGGTTCTTTAAGT
GATGGCGAATTTTCATCACAAATGTTTCGAATAAATTTAAATGATGAAAATGAACAATGG
GAAGAAGTGAAACCAAGAGGTGGAAAATCTTTTGATTATCGAATTGTTGCTCATACAACT
GTCTACCATAAATCTACAAAATCACTCATCATTTATGGTGGAATTATTGCTGGTGTTGCA
AGATTTTCAAAACTATCTGATCGAATGTTTTCATTTAATCTCATGGACAATCATTGGAGT
GAAATTTTTTATCCCAGAAAAGATTCAGTTCCAAGAGAAAGAGCATTTCATACAGCTGTA
ATTTATGGTGATTATATGATTATTTTTGGTGGTTATTCACATCGACATAATAAGGAAGAA
ATTTGCTATGACAATCAAATGTATTTGTACAATCTTATGTGTCATTTATGGGTTAATAAG
GAAGTTCTTGGTGATCAAAAATACGGAAGAAAATTAGATGAAACTCGCAACATTCGCTAT
CCAAAACGTCAAGGAGTTTTTGCTCACACTGCAATGCTTCGTCGTAACAATCAACTTTTA
ATTCATGGCGGTT
>g16669.t3 Gene=g16669 Length=584
MKAKKRGKFQKINFCAIFHLSILIFVSKFNKIEAVGSCDRTRQVFEGVNYGEITDGLNSN
YTQDSHCEWLIKAENSSQFITLKFLSLKTECSYDYIFIYDGNSYSSSPLLGSFSGQTDPQ
DITATSGFMLILLYSDTNYNLDGFRATFSITNCLKNCSSNGLCVNHKCHCNSDYSGNDCS
VKICKNCANEKLSSNESQWVWITQNYNSFTPRTAHIAIYDDVTDAVYTFGGYDLNNILGK
LEIYQFNDSKWIDEDEKEISIGIKNDNDEFWFRDALLAHVNSKRNKSNLNLHENEKIQIS
SEKAQNNQRNLKSINYESQKFKPLPRYGHAACKFKDSFLIYGGKLYNGSLSNELWMFNIS
TKIWSLKAKNSSLIPPKLTRHTLTFVNFNNFIYLFGGSLSDGEFSSQMFRINLNDENEQW
EEVKPRGGKSFDYRIVAHTTVYHKSTKSLIIYGGIIAGVARFSKLSDRMFSFNLMDNHWS
EIFYPRKDSVPRERAFHTAVIYGDYMIIFGGYSHRHNKEEICYDNQMYLYNLMCHLWVNK
EVLGDQKYGRKLDETRNIRYPKRQGVFAHTAMLRRNNQLLIHGG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g16669.t3 | CDD | cd00041 | CUB | 44 | 150 | 4.71217E-20 |
| 15 | g16669.t3 | Gene3D | G3DSA:2.60.120.290 | - | 38 | 154 | 2.0E-21 |
| 14 | g16669.t3 | Gene3D | G3DSA:2.120.10.80 | - | 279 | 546 | 3.0E-20 |
| 4 | g16669.t3 | PANTHER | PTHR10574 | NETRIN/LAMININ-RELATED | 34 | 186 | 1.6E-86 |
| 8 | g16669.t3 | PANTHER | PTHR10574:SF407 | - | 34 | 186 | 1.6E-86 |
| 5 | g16669.t3 | PANTHER | PTHR10574 | NETRIN/LAMININ-RELATED | 199 | 419 | 1.6E-86 |
| 9 | g16669.t3 | PANTHER | PTHR10574:SF407 | - | 199 | 419 | 1.6E-86 |
| 7 | g16669.t3 | PANTHER | PTHR10574 | NETRIN/LAMININ-RELATED | 420 | 538 | 1.6E-86 |
| 11 | g16669.t3 | PANTHER | PTHR10574:SF407 | - | 420 | 538 | 1.6E-86 |
| 6 | g16669.t3 | PANTHER | PTHR10574 | NETRIN/LAMININ-RELATED | 551 | 584 | 1.6E-86 |
| 10 | g16669.t3 | PANTHER | PTHR10574:SF407 | - | 551 | 584 | 1.6E-86 |
| 3 | g16669.t3 | Pfam | PF00431 | CUB domain | 58 | 148 | 2.9E-18 |
| 2 | g16669.t3 | Pfam | PF18720 | Tenascin EGF domain | 156 | 180 | 6.4E-7 |
| 1 | g16669.t3 | Pfam | PF13418 | Galactose oxidase, central domain | 325 | 365 | 1.3E-6 |
| 16 | g16669.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 11 | - |
| 18 | g16669.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 12 | 29 | - |
| 17 | g16669.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 30 | 584 | - |
| 21 | g16669.t3 | ProSiteProfiles | PS01180 | CUB domain profile. | 38 | 151 | 17.276 |
| 20 | g16669.t3 | SMART | SM00042 | CUB_2 | 38 | 151 | 2.9E-16 |
| 13 | g16669.t3 | SUPERFAMILY | SSF49854 | Spermadhesin, CUB domain | 59 | 153 | 1.2E-21 |
| 12 | g16669.t3 | SUPERFAMILY | SSF117281 | Kelch motif | 322 | 584 | 1.96E-32 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed